Microbiology of Textile Fibres V. Method for the General Histological Examination of Normal or Mildewed Cotton Fibres

Abstract

A method t is presented for detecting and observing changes in the histological sturcture of cotton fibres during the course of biological mildew devleopment. Fibre elements are first stained by the use of a 0.15% aqueous solution of Victoria Blue B. Swelling of the dyed fibre in cuprammonium solution containing not more than 0.8% copper is then used to accentuate and separate the finer structures for microscopic observation. The appearance of typical samples of normal raw cotton and badly mildewed raw cotton are shown in a series of eight photomicrographs.

Mildew- and Rot-Resistance of Textiles

“…Clegg [29] presents several photomicrographs showing the results of tests on variously damaged cotton fibers by use of the Congo red test. Prindle [99] has used a combination swelling-staining technique with cuprammonium solution and Victoria blue stain for detection of mildew damage to cotton fibers. Techniques described for staining fungus mycelium in cotton fibers include the use of cotton blue [53] and of basic fuchsin [108].…”