Microbiology of airway disease in a cohort of patients with Cystic Fibrosis

Lambiase, Antonietta; Raia, Valeria; Pezzo, Mariassunta Del; Sepe, Angela; Carnovale, Vincenzo; Rossano, Fabio

doi:10.1186/1471-2334-6-4

Cited by 99 publications

(64 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Additional studies are needed that examine the role of P. aeruginosa infection and resultant HMGB1 release, to better define this pathway in CF inflammation. Recent reports by Tian and colleagues (53) and Rouhiainen and colleagues (54) indicate that immune activation and cytokine release induced by HMGB1 is significantly enhanced after tight binding to bacterial DNA, prokaryotic lipids, and other bacterial substances that are present in high quantities in infected and colonized CF sputum (55,56). Not surprisingly, whereas blocking HMGB1 signaling caused a significant reduction in chemotaxis, our in vitro observations indicate that this is not the only pathway relevant to neutrophil influx (z75% of neutrophil influx remained unabated) and may indicate the importance of other inflammatory mediators such as IL-8 or PGP in these specimens.…”

Section: Discussionmentioning

confidence: 99%

Potential Role of High-Mobility Group Box 1 in Cystic Fibrosis Airway Disease

Rowe

Jackson

Liu

et al. 2008

Am J Respir Crit Care Med

100

View full text Add to dashboard Cite

Rationale: High-mobility group box 1 (HMGB1) is a potent inflammatory mediator elevated in sepsis and rheumatoid arthritis, although its role in cystic fibrosis (CF) lung disease is unknown. Objectives: To determine whether HMGB1 contributes to CF lung inflammation, including neutrophil chemotaxis and lung matrix degradation. Methods: We used sputum and serum from subjects with CF and a Scnn1b-transgenic (Scnn1b-Tg) mouse model that overexpresses bepithelial Na 1 channel in airways and mimics the CF phenotype, including lung inflammation. Human secretions and murine bronchoalveolar lavage fluid (BALF) was assayed for HMGB1 by Western blot and ELISA. Neutrophil chemotaxis was measured in vitro after incubation with human neutrophils. The collagen fragment prolineglycine-proline (PGP) was measured by tandem mass spectroscopy. Measurements and Main Results: HMGB1 was detected in CF sputum at higher levels than secretions from normal individuals. Scnn1b-Tg mice had elevated levels of HMGB1 by Western blot and ELISA. We demonstrated that dose-dependent chemotaxis of human neutrophils stimulated by purified HMGB1 was partially dependent on CXC chemokine receptors and that this could be duplicated in CF sputum and BALF from Scnn1b-Tg mice. Neutralization by anti-HMGB1 antibody, in both the sputum and BALF-reduced chemotaxis, which suggested that HMGB1 contributed to the chemotactic properties of these samples. Intratracheal administration of purified HMGB1 induced neutrophil influx into the airways of mice and promoted the release of PGP. PGP was also elevated in Scnn1b-Tg mice and CF serum. Conclusions: HMGB1 expression contributes to pulmonary inflammation and lung matrix degradation in CF airway disease and deserves further investigation as a biomarker and potential therapeutic target.

show abstract

Section: Discussionmentioning

confidence: 99%

Potential Role of High-Mobility Group Box 1 in Cystic Fibrosis Airway Disease

Rowe

Jackson

Liu

et al. 2008

Am J Respir Crit Care Med

100

View full text Add to dashboard Cite

show abstract

“…Pseudomonas aeruginosa, the archetypical CF pathogen, infects 70% of patients (1)(2)(3). The acquisition of this organism and its conversion to a hyper-alginate-producing mucoid phenotype are associated with deteriorating clinical status, increased treatment requirements, and progression to end-stage disease (4)(5)(6)(7)(8).…”

mentioning

confidence: 99%

Twenty-Five-Year Outbreak of Pseudomonas aeruginosa Infecting Individuals with Cystic Fibrosis: Identification of the Prairie Epidemic Strain

et al. 2014

View full text Add to dashboard Cite

Transmissible strains of Pseudomonas aeruginosa have been described for cystic fibrosis (CF) and may be associated with a worse prognosis. Using a comprehensive strain biobank spanning 3 decades, we sought to determine the prevalence and stability of chronic P. aeruginosa infection in an adult population. P. aeruginosa isolates from sputum samples collected at initial enrollment in our adult clinic and at the most recent clinic visit were examined by a combination of pulsed-field gel electrophoresis and multilocus sequence typing and compared against a collection of established transmissible and local non-CF bronchiectasis (nCFB) isolates. A total of 372 isolates from 107 patients, spanning 674 patient-years, including 66 patients with matched isolates from initial and final encounters, were screened. A novel clone with increased antibacterial resistance, termed the prairie epidemic strain (PES), was found in 29% (31/107 patients) of chronically infected patients referred from multiple prairie-based CF centers. This isolate was not found in those diagnosed with CF as adults or in a control population with nCFB. While 90% (60/66 patients) of patients had stable infection over a mean of 10.8 years, five patients experienced strain displacement of unique isolates, with PES occurring within 2 years of transitioning to adult care. PES has been present in our cohort since at least 1987, is unique to CF, generally establishes chronic infection during childhood, and has been found in patients at the time of transition of patients from multiple prairie-based CF clinics, suggesting broad endemicity. Studies are under way to evaluate the clinical implications of PES infection.

show abstract

“…In addition, in some circumstances, misidentification is due to the fact that the species are not in the database of commercial kits (10,23). Molecular tools such as 16S rRNA gene sequencing provide reliable results (10,16,26). Other techniques, such as fluorescence in situ hybridization (27) and amplified ribosomal DNA restriction assays, are available (22).…”

mentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cystic Fibrosis Patients

et al. 2008

View full text Add to dashboard Cite

The identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis (CF) patients is usually achieved by using phenotype-based techniques and eventually molecular tools. These techniques remain time-consuming, expensive, and technically demanding. We used a method based on matrixassisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) for the identification of these bacteria. A set of reference strains belonging to 58 species of clinically relevant nonfermenting gram-negative bacilli was used. To identify peaks discriminating between these various species, the profile of 10 isolated colonies obtained from 10 different passages was analyzed for each referenced strain. Conserved peaks with a relative intensity greater than 0.1 were retained. The spectra of 559 clinical isolates were then compared to that of each of the 58 reference strains as follows: 400 Pseudomonas aeruginosa, 54 Achromobacter xylosoxidans, 32 Stenotrophomonas maltophilia, 52 Burkholderia cepacia complex (BCC), 1 Burkholderia gladioli, 14 Ralstonia mannitolilytica, 2 Ralstonia pickettii, 1 Bordetella hinzii, 1 Inquilinus limosus, 1 Cupriavidus respiraculi, and 1 Burkholderia thailandensis. Using this database, 549 strains were correctly identified. Nine BCC strains and one R. mannnitolilytica strain were identified as belonging to the appropriate genus but not the correct species. We subsequently engineered BCC-and Ralstonia-specific databases using additional reference strains. Using these databases, correct identification for these species increased from 83 to 98% and from 94 to 100% of cases, respectively. Altogether, these data demonstrate that, in CF patients, MALDI-TOF-MS is a powerful tool for rapid identification of nonfermenting gram-negative bacilli.

show abstract

Microbiology of airway disease in a cohort of patients with Cystic Fibrosis

Cited by 99 publications

References 29 publications

Potential Role of High-Mobility Group Box 1 in Cystic Fibrosis Airway Disease

Potential Role of High-Mobility Group Box 1 in Cystic Fibrosis Airway Disease

Twenty-Five-Year Outbreak of Pseudomonas aeruginosa Infecting Individuals with Cystic Fibrosis: Identification of the Prairie Epidemic Strain

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cystic Fibrosis Patients

Contact Info

Product

Resources

About