Microbiology illuminated: gene engineering and bioluminescence

Stewart, Gordon S. A. B.; Denyer, Stephen Paul; Lewington, J.

doi:10.1016/0924-2244(91)90599-e

Cited by 19 publications

(12 citation statements)

References 5 publications

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“…The physiological effects of iron, oxygen and osmolarity on luminescence are only partially understood but can affect the accuracy of the bioassay (17,53). The effects of endogenous aldehyde and internal ions on bioluminescence expression of P. phosphoreum have been well documented (26,56,61).…”

Section: The Microtox Systemmentioning

confidence: 99%

“…These applications include construction of biosensors for detection of contaminants, measurement of pollutant toxicity (44), and monitoring of genetically engineered bacteria released into the environment (15,33,48). Biosensors have also been used as indicators of cellular metabolic activity (57) and for detection of pathogens (9,53). The use and limitations of bioluminescence as reporter systems for pollutants from environmental samples are discussed below.…”

Section: Applications Of Bioluminescence For Environmental Microbiologymentioning

confidence: 99%

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Bioluminescent bacteria: lux genes as environmental biosensors

Nunes-Halldorson¹,

Duran²

2003

Braz. J. Microbiol.

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Bioluminescent bacteria are widespread in natural environments. Over the years, many researchers have been studying the physiology, biochemistry and genetic control of bacterial bioluminescence. These discoveries have revolutionized the area of Environmental Microbiology through the use of luminescent genes as biosensors for environmental studies. This paper will review the chronology of scientific discoveries on bacterial bioluminescence and the current applications of bioluminescence in environmental studies, with special emphasis on the Microtox toxicity bioassay. Also, the general ecological significance of bioluminescence will be addressed.

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Section: The Microtox Systemmentioning

confidence: 99%

Section: Applications Of Bioluminescence For Environmental Microbiologymentioning

confidence: 99%

Bioluminescent bacteria: lux genes as environmental biosensors

Nunes-Halldorson¹,

Duran²

2003

Braz. J. Microbiol.

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“…Use of low-light imaging systems also allows us to elucidate, for the first time, the spatial distribution of preservative action within complex food matrices, where previous analysis techniques required disruption of the food matrix prior to bacterial enumeration. By either incorporating lux' bacteria into the foodstuff or overlaying food sections with indicator bacteria, regions with preservative or biocidal activity may be identified by the local depression of bioluminescence (6,32).…”

Section: A Reporter Of Bacterial Viabilitymentioning

confidence: 99%

Use of lux genes in applied biochemistry

Hill

Stewart

1994

J. Biolumin. Chemilumin.

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Bioluminescence has emerged in the last decade as a major tool for the study of bacterial adaptation and survival. In addition to the advantages of sensitivity and the real-time, non-invasive nature of this reporter, the imaging potential of using low-light and photon-counting video cameras has been particularly influential in establishing its ascendancy-over more traditional reporter systems. This review provides a reflection of personal activity in this field through applications in Food Microbiology and collaboration with colleagues both in the UK and beyond.

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“…The importance of in vivo bioluminescence as a reporter of cellular viability has been the subject of several reviews (Stewart, 1990(Stewart, , 1993Stewart & Williams, 1992, 1993Stewart et al, 1991Stewart et al, , 1993Stewart et al, , 1996Stewart et al, , 1997aHill et al, 1993a, b;Ellison et al, 1994b). Given that the production of light from recombinant bacteria containing the lux genes depends upon a functional intracellular biochemistry, it can be established that any substance or environment that impairs that biochemistry, and thus compromises cellular viability, will lead to a reduction in light emission.…”

Section: Bade R Ia I Via Bi I I Tymentioning

confidence: 99%

Challenging food microbiology from a molecular perspective

Stewart

1997

Microbiology

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Summary: Two key themes within food microbiology are bacterial detection and control. There is a raft of sub-headings under each of these themes, but in the last decade molecular approaches within each have made a significant contribution to the field. This is a personal review of the author’s past and present contributions and future ideas for challenging food microbiology from a molecular perspective.

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Microbiology illuminated: gene engineering and bioluminescence

Cited by 19 publications

References 5 publications

Bioluminescent bacteria: lux genes as environmental biosensors

Bioluminescent bacteria: lux genes as environmental biosensors

Use of lux genes in applied biochemistry

Challenging food microbiology from a molecular perspective

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