Microbiological status of rabbit carcases in Egypt

Khalafalla, F. A.

doi:10.1007/bf01202738

Cited by 13 publications

(16 citation statements)

References 12 publications

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“…So that slaughtered rabbit carcasses generally have low bacterial count when slaughtered under high sanitary conditions, while during handling and processing the number of microorganisms increased significantly. Moreover, these reports are in accordance with the conclusion of Khalafalla (1993) that slaughtered rabbit carcasses from the groceries stores have higher bacterial counts than home slaughtered.…”

Section: Apc At 35 °Csupporting

confidence: 89%

“…It was found that it ranged from 5.1×10 3 to 8.8×10 6 , 3.9×10 3 to 9.3×10 6 , 2.6×10 3 to 9.3×10 6 and 5.1×10 3 to 4.0×10 6 , with mean values accounted for 1.5×10 6 , 1.1×10 6 , 1.2×10 6 and 5.7×10 5 CFU/g flesh in shoulder, loin, rib and thigh regions, respectively. Slightly higher results were recorded by Abou-Taleb (1995) and Hohn (1960), while approximately similar values were obtained by Shiffman (1961) and Sunki et al (1978), and lower values were reported by Comin et al (2008) and Khalafalla (1993). From the data illustrated in Table (2), it could be concluded that the MPN of coliforms in examined rabbit meat samples ranged from <3 to 1.5×10 4 , <3 to 9.3×10 3 , <3 to 1.5×10 4 and 7.4 to 9.3×10 3 , with mean values of 2.0×10 3 , 1.2×10 3 , 1.7×10 3 and 1.7×10 3 microorganisms/g meat in shoulder, loin, rib and thigh regions, respectively.…”

Section: Resultssupporting

confidence: 51%

“…The microbiological quality of rabbit carcasses and changes during processing have not been studied in such depth as for other meats, accordingly, information on the microbiological quality of rabbit meats in Egypt is scarcely found (Abou-Taleb, 1995;Ali et al, 2015 andKhalafalla, 1993). Therefore, the current study was designed in order to evaluate the bacterial profile of rabbit carcasses marketed in Beni-Suef province through the determination of the aerobic plate count (APC) at 35 °C, and most probable number (MPN) of coliforms, faecal coliforms and E. coli.…”

Section: Introductionmentioning

confidence: 99%

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Bacterial Profile of Retail Rabbit Carcasses Marketed in Beni-Suef Province, Egypt

2016

Assiut Veterinary Medical Journal

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The current study aimed to evaluate the bacteriological status of retail rabbit carcasses marketed in Beni-Suef province, Egypt. For such aim, a total of 25 fresh rabbit carcasses were randomly collected from different rabbit markets in Beni-Suef during 2015. The collected samples were subjected to determination of aerobic plate count (APC) at 35 °C, and most probable number (MPN) of coliforms, faecal coliforms and E. coli, in addition to isolation and identification of E. coli (true faecal type), Salmonella spp. and Yersinia enterocolitica. The obtained results revealed that 32, 64, 72 and 52 % of examined rabbit meat samples from shoulder, loin, rib and thigh regions, respectively, exceeded the acceptable limits recommended by Egyptian standards for APC (10 5 CFU/g flesh). While none of the examined samples exceeded the international standards (10 7 CFU/g) stated by the International Commission on Microbiological Specification for Foods (ICMSF). Regarding the pathogenic microorganisms, it was found that 11 (44 %), 8 (32 %), 15 (60 %) and 10 (40 %) out of 25 rabbit cuts contained E. coli biotype I in shoulder, loin, rib and thigh regions, respectively. However, 3 (12%), 3 (12%), 3 (12%) and 2 (8%) samples contained Salmonella spp., and 3 (12%), 3 (12%), 6 (24%) and 7 (28%) contained Yersinia enterocolitica, respectively. The public health significance of isolated pathogens and their sources of contamination were discussed throughout the study.

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Section: Apc At 35 °Csupporting

confidence: 89%

Section: Resultssupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

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Bacterial Profile of Retail Rabbit Carcasses Marketed in Beni-Suef Province, Egypt

2016

Assiut Veterinary Medical Journal

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“…For frozen thighs from Argentinian hare, Hoppe (1981) reported a range of 3 to 5 log cfu/g, and Türck (2008) reported a median of 3.7 log cfu/g (range of 2.2 to 6.2 log cfu/g). It is important to note that hunted hares are not necessarily more contaminated than slaughter animals of similar size, e.g., mean values of 4-5 log cfu/g for slaughtered farmed rabbits are reported by several studies conducted on different continents (Khalafalla 1993;Bobbitt 2003;Rodriguez-Calleja et al 2004). Initial EB counts were 2.2±0.3 and 2.7±0.2 log cfu/g (n= 10) for categories "A" and "B", respectively.…”

Section: Development Of the Microflora And Phmentioning

confidence: 95%

Lesions in thighs from hunted Brown Hares (Lepus europaeus) and microflora under vacuum-packaging storage

et al. 2010

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In two surveys, thighs of a total of 137 hunted hares were tested for the presence of intramuscular shots and femur fractures, which were detected in 42.7% and 29.2% of 274 thighs, respectively. Femur fractures were significantly associated with the presence of intramuscular shots. In the second survey (46 hares), 92 thighs were grouped into three categories, "A" (no fractures, no intramuscular shot), "B" (one intramuscular shot), and "C" (multiple shots and hematoma), with 49.0%, 33.6%, and 17.4%, respectively. Category "C" was found unfit for human consumption. During 7-day storage of vacuumpacked "A" and "B" thighs, total aerobic counts increased from initially 3.3±0.3 (mean±SD) and 4.1±0.6 log cfu/g by ca. 2 log units when stored at 3-4°C, whereas the increase was clearly <1 log unit at 0°C. In comparison to temperature, differences between "A" and "B" category were less pronounced. Similar dynamics were observed for Enterobacteriaceae. In all categories, muscle pH values (mean=5.83) were similar. It is concluded that storage at temperatures of ca. 4°C, although in compliance with EU legislation, does not afford keeping microbial contaminants in check, and thus will not preserve microbiological quality of vacuum-packed hare meat.

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“…Similarly, to meat of other food animals intended for human consumption, rabbit meat should be free from any microbiological hazards. Different types of bacterial pathogens are nevertheless sporadically detected in raw rabbit meat (Khalafalla 1993;Pyz-Łukasik and Szkucik 2005).…”

Section: Introductionmentioning

confidence: 99%

Application of ELISA recomWell HEV IgG (Human) for Detection of Virus-Specific Antibodies in Sera of Slaughtered Rabbits

Bigoraj

Rzeżutka

2018

Food Anal. Methods

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HEV infections in animals are mainly diagnosed based on the presence of anti-HEV antibodies in sera using enzyme-linked immunosorbent assays (ELISA). In fact, commercial HEV ELISA kits are only available for selected animal species excluding rabbits. The demonstration of cross-reactivity of rabbit anti-HEV antibodies with human HEV strains makes possible diagnostic use of (human) ELISA kits for detection of specific anti-HEVantibodies in rabbit sera. The aim of the study was the application of ELISA recomWell HEV IgG (human) for identification of HEV seropositive rabbits at the time of slaughter. Native recombinant protein A was used as broadly reactive conjugate characterized by cross-species reactivity with IgG antibodies of human and other animal species. Several dilutions of protein A were employed and subsequently tested against the panel of control sera (positive, negative, and borderline) to determine its optimal concentration. Statistical analysis of OD values and their corresponding antibody titers obtained for each group of tested sera and protein A showed the most consistent performances at 1:40,000 dilution when compared to anti-human IgG (control) conjugate. Finally, the correct performance of the optimized test was verified using rabbit serum samples containing (or not) specific anti-HEV antibodies. As demonstrated here, HEV IgG (human) ELISA employing ORF2 antigens of human gt1 and gt3 HEV strains after modification and subsequent optimization can be used for the detection of anti-HEV antibodies in rabbit sera.

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Microbiological status of rabbit carcases in Egypt

Cited by 13 publications

References 12 publications

Bacterial Profile of Retail Rabbit Carcasses Marketed in Beni-Suef Province, Egypt

Bacterial Profile of Retail Rabbit Carcasses Marketed in Beni-Suef Province, Egypt

Lesions in thighs from hunted Brown Hares (Lepus europaeus) and microflora under vacuum-packaging storage

Application of ELISA recomWell HEV IgG (Human) for Detection of Virus-Specific Antibodies in Sera of Slaughtered Rabbits

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