Microbiological Screening of Hypothermic Preserved Donor Corneas in Keratoplasty

Li, Saiqun; Zhong, Jing; Tan, Yiwei; Deng, Yuqing; Huang, Haixiang; Wang, Bowen; Peng, Lulu; Zhang, Henan; Yuan, Jing

doi:10.1080/02713683.2019.1616766

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…Finally, given the worldwide shortage of cornea donations, Gram stain should be adopted for rapid identification of microbial contaminants. This would aid surgeons in selecting appropriate preventive antibiotics and antifungal drugs, though grafts with a positive rim culture do not tend to result in postoperative infections (Li et al 2019a , b ).…”

Section: Discussionmentioning

confidence: 99%

“…Although storage solutions contain empiric broad-spectrum antibiotics, organ culture is still subject to microbial contamination due to media composition and conditions conducive to growth: temperature between 30 and 37 °C and length of preservation time (Thareja et al 2020 ). The addition of antibiotics to storage medium is an unreliable remedy due to the increasing antibiotic resistance of contaminating microbes (Li et al 2019a , b ).…”

Section: Introductionmentioning

confidence: 99%

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Gram stain and addition of amphotericin B to improve the microbial safety of human donor corneas

et al. 2021

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To determine the effectiveness of two methods to improve the microbial safety of human corneas preserved in organ culture. We compared the number of positive preservation solutions of corneas in organ culture in which the initial short-term hypothermic corneal maintenance solution was supplemented with amphotericin B 2.5 µg/mL and the historical data of microbial test results (2015–2019). In addition, we appraised the efficacy of Gram stain to detect bacterial or fungal contamination in the organ culture solutions of corneas from at-risk donors compared to the culture tests of corneas from not-at-risk donors. Statistical analysis was performed using STATA and statistical significance set at p < 0.05. The number of positive culture tests after preservation was 15 (0.5%) in 2020 compared to a mean of 37 (1.2%) in the period 2015–2019 (p < 0.01), with 10 (1.0%) positive samples in the cohort of 998 corneas from at-risk donors and 5 (0.2%) in the 2046 corneas from not-at-risk donors (p < 0.01). All corneas from at-risk donors tested positive at Gram stain and the results were available 1–3 days before those of the conventional culture tests. Amphotericin B supplementation in the short-term maintenance solution markedly reduced the number of positive microbial tests after organ culture and the early detection of contaminants, including slow-growing microorganisms, by Gram stain before the standard culture results. This meant fewer corneas being discarded and a greater likelihood of preventing post-graft infections.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gram stain and addition of amphotericin B to improve the microbial safety of human donor corneas

et al. 2021

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“…These results are quite similar to other series (Gruenert et al 2017 , Li et al 2019 ; Ling et al 2019 ). Li et al ( 2019 ) reported 8.2% of positive cultures in 111 donor corneas, most common bacteria and fungi being Acinetobacter baumannii complex (19.8%) and Candida spp . (9.0%), respectively.…”

Section: Introductionmentioning

confidence: 99%

Clinical outcomes of long-term corneas preserved frozen in Eusol-C used in emergency tectonic grafts

et al. 2022

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To report the clinical results on the use of corneas frozen in Eusol-C as tectonic corneal grafts.Retrospective review of medical records of patients who received frozen corneas as emergency tectonic grafts from 2013 to 2020. Corneas had been stored in Eusol-C preservation media at − 78 °C for a mean time of 6.9 months. Diagnosis, transplant characteristics, microbial culture results, anatomic integrity, epithelial healing, neovascularization, transparency, infection and need for additional surgeries were registered. Fifty corneas were used in 40 patients (mean age 60.5 years, 20 males) with a median follow-up of 27.3 months after surgery. Need for tectonic graft was due to: perforation secondary to immune diseases (6, 12%), neurotrophic ulcer (11, 22%), trauma (3, 6%), corneal infection (11, 22%), chronic disorders of the ocular surface (9, 18%) and previous corneal graft failure (10, 22%). Mean size of grafts was 5.6 mm and 36 cases (72%) also received an amniotic membrane graft. Thirty-eight corneas achieved epithelization (76%), 25 (50%) were clear and 19 (38%) developed neovascularization. None of the corneas were rejected. Seventeen corneas (34%) failed: 7 (14%) due to reactivation of baseline disease and 10 (20%) due to primary graft failure. Four corneas (8%) had positive microbial cultures suggestive of contamination and 2 (4%) developed a cornea abscess non-related to a positive microbial culture. Long-term preservation of donor corneas in Eusol-C at − 78 °C is a viable technique to meet the needs of emergency grafts with minimal equipment.

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Microbiological screening of corneas stored in organ culture medium at Lions Eye Bank of Western Australia from 2011 to 2022

Kezic,

Buckland,

Wiffen

2024

Int Ophthalmol

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Microbiological Screening of Hypothermic Preserved Donor Corneas in Keratoplasty

Cited by 5 publications

References 37 publications

Gram stain and addition of amphotericin B to improve the microbial safety of human donor corneas

Gram stain and addition of amphotericin B to improve the microbial safety of human donor corneas

Clinical outcomes of long-term corneas preserved frozen in Eusol-C used in emergency tectonic grafts

Microbiological screening of corneas stored in organ culture medium at Lions Eye Bank of Western Australia from 2011 to 2022

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