Microbiological quality of raw and processed farm reared periwinkles from brackish water earthen pond Buguma, Nigeria

Omenwa, VC; Ansa, EJ; Agokei, O.E.; Uka, A; George, OS

doi:10.4314/ajfand.v11i2.65917

Cited by 10 publications

(11 citation statements)

References 8 publications

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“…This could be due to the negative effect of heat on the growth of bacterial contaminants. Regarding the frequency of bacterial isolate from heat treated fish and other ready-to-eat food stuffs similar observations were reported by other researchers (Mezgebu and Mogessie, 1998;Thailambal, 2006;Omenwa et al, 2012). Finding of this study indicated that 42.5% of frozen raw Nile tilapia fillet samples carried E. coli.…”

Section: Total Plate Count (Tpc)supporting

confidence: 78%

Microbiological quality of frozen raw and undercooked Nile tilapia (Oreochromis niloticus) fillets and food safety practices of fish handlers in Arba Minch town, SNNPR, Ethiopia

Teka¹,

NÃ¶lkes²,

Getachew³

et al. 2017

J. Vet. Med. Anim. Health

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The study was designed to assess the microbiological quality of Nile tilapia (Oreochromis niloticus) fillets and food safety practices of fish handlers in Arba Minch town, SNNPR, Ethiopia. A questionnaire survey was conducted to assess the food safety practices of fish handlers. The microbiological quality of frozen raw and undercooked (Asa leb-leb) tilapia fillets was assessed by determining the total plate count and isolating hygiene indicator bacteria that is, Escherichia coli and other major foodborne pathogens that is, Salmonella spp. and Staphylococcus aureus. Among interviewed fish handlers, 32.6% and 55.8% were not found to use overcoat and hair cover, respectively. Moreover, 30.2% of the fish handlers didn't take training regarding sanitary handling of fish food. The highest mean bacterial count was observed in frozen raw tilapia fillet sample (4.63x106 cfu/g), which was significantly (p=0.037) different from undercooked tilapia fillet (4.92x103 cfu/g). From 40 frozen raw samples of tilapia fillet examined, 42.5% had E. coli, 7.5% had Salmonella spp. and 65% had S. aureus. In contrast, from 40 undercooked samples of tilapia fillet examined, 7.5% had E. coli and 17.5% had S. aureus whilst Salmonella spp. was not detected. A significant (p=0.000) difference in frequency of bacterial isolates were also observed between frozen raw and undercooked tilapia fillet. Generally, the study revealed that there was a gap on food safety practices and high microbial profile were observed. Hence, it could be wise to advice the government to improve the food safety practices and quality standards of fish sold in the town.

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Section: Total Plate Count (Tpc)supporting

confidence: 78%

Microbiological quality of frozen raw and undercooked Nile tilapia (Oreochromis niloticus) fillets and food safety practices of fish handlers in Arba Minch town, SNNPR, Ethiopia

Teka¹,

NÃ¶lkes²,

Getachew³

et al. 2017

J. Vet. Med. Anim. Health

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“…On boiling the bacteria in the periwinkles without shells were significantly lower than those in the boiled periwinkles with shells (P<0.05). This result is in consonance with the work of Omenwa et al [20]. [28].…”

Section: Discussionsupporting

confidence: 94%

“…Group 1: Periwinkle Samples with Shells:-At the laboratory, the periwinkle samples with shells were extensively scrubbed, washed and rinsed using normal saline solution to remove dirt, debris and surface contaminants [19]. The pointed ends were cut off using a sterile knife [20]. All aseptic techniques were carried out under the Purifier Biosafety Cabinet (Model Delta series, LABCONCO, USA).…”

Section: The Samples Were Divided Into Four Groupsmentioning

confidence: 99%

Antibiogram of Bacteria Isolated from Tympanotonus fuscatus Var. Radula (Prosobranchia:Potamididae) Sold in Markets in Nasarawa State, Nigeria

Asemota¹,

Makut

Obiekezie

et al. 2020

SAJRM

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The aim of this study was to determine the antibiogram of bacterial isolates from Tympanotonus fuscatus var. radula sold in markets in Nasarawa State. Nigeria. Samples of Tympanotonus fuscatus var. radula (periwinkles) were bought from soup ingredient sellers at different sale locations in Keffi, Masaka and Orange markets and were analyzed using standard bacteriological methods. The bacterial isolates were identified using morphological, cultural and biochemical techniques. The total bacteria count varied from 1.18–3.20 x 108 CFU/g for the raw samples while the total bacterial count for the boiled samples varied from 0–1.57 x 108 CFU/g. Periwinkle samples with shells from Masaka market had the highest bacterial load with a mean total bacterial count of 2.94 x 10⁸ CFU/g and mean total coliform count of 2.80 x 10⁶ CFU/g. Raw periwinkle samples with shells had a higher bacterial load than samples without shells. There was also a drastic reduction in the bacterial load in the periwinkle samples after boiling under laboratory conditions. The bacteria isolated were Bacillus spp. and Staphylococcus aureus were the Gram-positive bacteria isolated. Enterobacter spp., Escherichia coli, Salmonella spp., Pseudomonas spp., Serratia spp. and Proteus spp. The most frequently occurring gram positive bacteria was Escherichia coli with an isolation frequency of 6(24%), the least frequently occurring was Bacillus spp., 8(32)%. Antibiotic susceptibility test showed that all the gram negative organisms exhibited sensitivity to ciprofloxacin: Escherichia coli (32 mm), Enterobacter spp. (41.5 mm), Proteus spp. (40.0 mm), Salmonella spp. (37.0 mm), Serratia spp. (26.0 mm), Pseudomonas spp. (23.0 mm). All the gram negative organisms showed marked resistance to vancomycin: Escherichia coli (12.0 mm), Enterobacter spp. (10.0 mm), Proteus spp. (11.0 mm), Salmonella spp. (5.0 mm), Serratia spp. (10.0 mm) and Pseudomonas spp. (4.5 mm).

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“…This result is also comparable with that of Zimmerman et al [20], in the Gulf of Mexico waters. Vibiro species were also isolated from Buguma, but at lower concentrations, this supports the findings of Omenwa et al [21], in the same creek. This may be due to human activities, such as bathing, defecation by the local populace, coupled with waste from cottage industries, which are common phenomenon in the area.…”

Section: Discussionsupporting

confidence: 90%

Seasonal Changes of Microbial Load in Some Sea Foods from Buguma and Ekerekana Creeks, Niger Delta, Nigeria

Edun¹,

Akinrotimi²,

Makinde³

2016

Ann Environ Sci Toxicol

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Background and Aim: Niger delta environment has been exposed to organic and inorganic contaminants from industries and domestic wastes, thereby enhancing the capacity of the ecosystem into harboring a sizeable population of microorganisms. Most of these microorganisms are found in the water column, bottom sediment and water film, resulting in their elevated levels in seafood. Proper understanding of the transfer of microorganisms through the food web is essential to predict the exposure of seafood consumers to possible health consequences associated with its consumption in the coastal areas. Hence, the present study examines the microbial content of some sea foods such as: periwinkle (Tympanotonus fuscatus); mudskipper (Periophthalmus papilio); mullets (Liza falcipinis) Oyster (Crassotrea gasar) and water from Ekerekana and Buguma creeks, in the Niger delta, Nigeria. Methods: Seasonal variations in microbial content in some seafood namely: Periwinkle (T. fuscatus); mudskipper (P. papilio); mullet (L. falcipinnis) and Oyster (C. gasar) from Ekerekana and Buguma creeks, Niger delta, Nigeria were assessed. The sea foods were sampled monthly from both creeks for a period of eight months. Standard methods were employed in the evaluation of microbial load in each species. Results: The result indicated that the microorganisms isolated: total heterotrophic bacteria count, total vibro count; and total coliform count were more predominant in the wet season when compared to dry season months. Total coliform count bacteria exhibited some measure of elevation in the dry season months, an indication that they can be prevalent in both seasons depending on the type of human activities that is prevalent in the area. Conclusion: This study has shown that industrial and domestic wastes discharged into Ekerekana and Buguma creek resulted in high concentrations of pollutants in the water body, which promotes the growth of microorganisms in the water column and sea foods, this may pose a health hazard to humans as a final consumer of these organisms There is therefore the need to formulate appropriate policies and regulations for safeguarding the ecosystem from adding undesirable microbial population. isolated from waters which contain fecal materials [8-10]. Pathogenic bacteria in marine waters are most abundant in the sediments, they can also be found on the surface film, as well as in the water column [11]. As a result, fin and shell fishes found in marine environment often show elevated levels of these bacteria, which can cause disease in fish as well as human host that consume them. In most Niger delta communities, periwinkle, oyster, mullets and mudskipper are delicacies, they are in high demand among the populace living in these areas [12,13]. However, proper understanding of the transfer of microorganisms through the food web is essential to predict the exposure of consumers of this seafood to possible health consequences associated with their consumption. A large proportion of people living in rural and urban communities of N...

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Microbiological quality of raw and processed farm reared periwinkles from brackish water earthen pond Buguma, Nigeria

Cited by 10 publications

References 8 publications

Microbiological quality of frozen raw and undercooked Nile tilapia (Oreochromis niloticus) fillets and food safety practices of fish handlers in Arba Minch town, SNNPR, Ethiopia

Microbiological quality of frozen raw and undercooked Nile tilapia (Oreochromis niloticus) fillets and food safety practices of fish handlers in Arba Minch town, SNNPR, Ethiopia

Antibiogram of Bacteria Isolated from Tympanotonus fuscatus Var. Radula (Prosobranchia:Potamididae) Sold in Markets in Nasarawa State, Nigeria

Seasonal Changes of Microbial Load in Some Sea Foods from Buguma and Ekerekana Creeks, Niger Delta, Nigeria

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