“…HPP offers an alternative potential non-thermal preservation method for processing food. The major benefit of pressure is its immediate and uniform effect throughout different media, avoiding complications such as non-stationary conditions typical for convection-type and conduction-type processes (Crawford, Murano, Olsen, & Shenoy, 1996;Knorr, 1993;O'Brien & Marshal, 1995;Patterson, Quinn, Simpson, & Gilmour, 1995;Raso, Gongora-nieto, Barbosa-Cánovas, & Swanson, 1998;Yuste, Mor-Mur, Capellas, Guamis, & Pla, 1998). Compared with the classical heat treatment, HPP treatment causes less deterioration of essential vitamins, phytochemicals, aroma compounds but microorganisms can be inactivated if the applied pressures are high enough (Cheftel, 1995;Hite, 1899 Our investigation of optimization of process conditions to inactivate Bacillus subtilis by high hydrostatic pressure and mild heat using response surface methodology showed that the optimum process parameters for a 6-log-cycles reduction of B. subtilis were obtained as temperature, 46°C; pressure, 479 MPa; and pressure holding time, 14 min (Gao & Jiang, 2005).…”