Microbiological quality of fresh vegetables and fruits sold in Singapore

Seow, J.; Ágoston, Réka; Phua, Leslie; Yuk, Hyun‐Gyun

doi:10.1016/j.foodcont.2011.10.017

Cited by 95 publications

(73 citation statements)

References 33 publications

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“…[1,14,17], Listeria monocytogenes [1,17,18,19], thermotolerant Campylobacter spp. [20,21], parasitic and viral pathogens capable of causing human illness and a number of reports refer to raw vegetables and fruits harbouring potential foodborne pathogens [3,13,16,[22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Microbiological Quality of Fresh Vegetables and Fruits Collected from Supermarkets in Istanbul, Turkey

Büyükünal¹,

Issa²,

Aksu³

et al. 2015

JFNS

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Abstract:Two hundred sixty one samples, collected from supermarkets in Istanbul, Turkey. All samples were analysed for aerobic mesophilic bacterial counts (AMC), aerobic psychrotrophic bacterial counts (APC), enumeration of yeasts and moulds (YM), coliforms, Escherichia coli and detection of Escherichia coli O157:H7, Salmonella spp., Listeria monocytogenes, thermotolerant Campylobacter spp. AMC ranged from 2.95 to 3.75 log 10 CFU/g. APC ranged from 0 to 3.55 log 10 CFU/g. The highest counts of coliforms were found in carrot, spinach, green leaf lettuce, cos lettuce and iceberg lettuce. The highest counts of YM were found in tomato, spinach, green leaf lettuce, cos lettuce and iceberg lettuce. Green leaf lettuces, cos lettuces, iceberg lettuces, spinach and carrot were highly contaminated with aerobic mesophilic (3.6 log 10 CFU/g), psychrotrophic microorganisms (3.4 log 10 CFU/g), and showed a high incidence of E.coli (41.77% of samples). Of the samples analysed, 10 (3.83%) were Salmonella spp. positive and 17 (6.51%) were thermotolerant Campylobacter spp. positive. None of the samples was positive for Escherichia coli O157:H7 and Listeria monocytogenes. Fresh vegetables and fruits, sold in Istanbul, Turkey, are needed to control foodborne pathogens especially Salmonellosis and Campylobacteriosis.

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Section: Introductionmentioning

confidence: 99%

Microbiological Quality of Fresh Vegetables and Fruits Collected from Supermarkets in Istanbul, Turkey

Büyükünal¹,

Issa²,

Aksu³

et al. 2015

JFNS

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“…Seow et al (2012) have showed that among several vegetables and fruits, raw and ready-to eat lettuce are more contaminated with high level of mesophilic bacteria and coliforms. Vegetables need to be well washed and disinfected before been processed.…”

Section: *Corresponding Author E-mail: Jc_kalpy@yahoofr Author(s) Amentioning

confidence: 99%

Microbiological quality of raw vegetables and ready to eat products sold in Abidjan (Cte dIvoire) markets

Julien¹,

Agbo²,

Dadié³

et al. 2017

Afr. J. Microbiol. Res.

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Vegetables are usually consumed raw. This implied best hygienic conditions from the harvest to the processing because of the gastro-enteritis that they could provoke. This study was conducted with the aim to appreciate microbiological quality of raw tomatoes, endives and ready-to-eat products sold in markets. Samples were taken randomly in two markets of Abidjan. A microbiological analysis was done in order to identify and enumerate faecal coliforms, Escherichia coli, Enterococcus and Pseudomonas. A decontamination treatment based on washing samples with running water and sodium hypochlorite solution I° chlorymetric was also applied to tomatoes and endives. The results indicated that, for tomatoes and endives, the average load was 1.5.10 4 CFU/g of Enterococcus, 1.3.10 3 CFU/g of Pseudomonas and 1.7.10 2 CFU/g of faecal coliforms. In ready-to-eat products, the load was 9.3.10 1 CFU/g for Enterococcus, l.03.10 1 CFU/g for Pseudomonas and 9.9.10 1 CFU/g for faecal coliforms. The disinfection with a sodium hypochlorite solution l° chlorymetric reduced Enterococcus and faecal coliforms load to 98% and Pseudomonas load to 97% as compared to the washing with running water in which Enterococcus was only reduced to 80%, faecal coliforms to78% and Pseudomonas to 73%. Escherichia coli were isolated in 28 samples as follow: 15 stumps from endives (54%), 10 stumps from tomatoes (36%) and 3 stumps from ready-to-eat products (10%). Results showed that before consumption, vegetables need to be washed, cleaned and disinfected. This will avoid sanitary hazard.

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“…Samples were collected as described by Seow et al [50], briely, personnel of the laboratory was transferred to the production site in the Yaqui Valley and sampled letuce from the process, were sampled in original package and immediately placed in sterile resealable bags, and later were transported with iceboxes and stored to 4°C until analysis. Product information such as production date, lote, and "best before" were registered in the database of the study.…”

Section: Transport and Handling Of Samplementioning

confidence: 99%

PCR Assay for Detection of Staphylococcus aureus in Fresh Lettuce (Lactuca sativa)

Chávez‐Almanza¹,

López‐Cervantes²,

Cantú-Soto³

et al. 2017

Frontiers in Staphylococcus Aureus

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The growth in food demand and production growth of vegetables have led to the development of intensive production systems with the aim of having regular access to enough high-quality food. The aim is to determine the incidence of Staphylococcus aureus in fresh letuce by PCR in order to enhance the eiciency for detection and identiication process. The Baird-Parker method was used for isolating pathogens from 54 letuce samples. Genomic DNA extraction was performed according the Mericon DNA Bacteria Plus Kit. The detection by PCR was performed using the pair of primers: coa gene (5′-ATAGAGCTGATGGTACAGG-3′ and 5′-GCTTCCGATTGTTCGATGC-3′). The phylogenetic tree was constructed by comparing conserved sequences from the adjacent 16S gene, using the F2C 5′-AGAGTTTGATCATGGCTC-3′ and C 5′-ACGGGCGGTGTGTAC-3′ primers. To test the antimicrobial efect, we used the disk difusion method (Kirby-Bauer) using Mueller-Hinton agar and ive antibiotics with diferent concentrations. The incidence of S. aureus was 1.7%. All the isolates were situated in the ATCC 11632 clade in accordance with other reported sequences belonging to this pathogen in the NCBI database. All the isolates seemed to be resistant to penicillin (10U). The molecular techniques used in this study are suitable for the identiication of S. aureus isolated from letuce, increasing our capability of detecting this pathogen by improving the process and increasing the eiciency contributing to the safety of this vegetable.

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Microbiological quality of fresh vegetables and fruits sold in Singapore

Cited by 95 publications

References 33 publications

Microbiological Quality of Fresh Vegetables and Fruits Collected from Supermarkets in Istanbul, Turkey

Microbiological Quality of Fresh Vegetables and Fruits Collected from Supermarkets in Istanbul, Turkey

Microbiological quality of raw vegetables and ready to eat products sold in Abidjan (Cte dIvoire) markets

PCR Assay for Detection of Staphylococcus aureus in Fresh Lettuce (Lactuca sativa)

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