Microbiological Profile of Urinary Tract Infections with special Reference to Antibiotic Susceptibility Pattern of Escherichia coli Isolates

Hegazy, Eman; Din, Rasha Abd El Hamid Alam El; Amin, Ahmed Mostafa; Mahgoub, Fawkia Mohamed; Gamal, Samir Abd El Hakeem El

doi:10.20546/ijcmas.2018.702.115

Cited by 7 publications

(2 citation statements)

References 17 publications

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“…Isolates of this type belong to the genus Staphylococcus aureus, while the number of isolates negative for gram-negative is 15 isolates by accepting the red safranin dye. It was found that this type of isolates belong to the genus E.coli, as they produce fermented pink colonies of lactose sugar on the MaCconkey agar medium, and they appeared in a shiny green color on the EMB medium.The results showed that the number of E.coli isolates reached 50%, and this percentage is close to studies conducted by researchers in Egypt and India (27) The percentage of isolation of these bacteria was 54.7% and 53.85%, respectively. The frequency of S. aureus bacterial isolation was also 50%, and this percentage is close to what was obtained by the researcher ( 28) as he isolated it by 55.9%.…”

Section: X-ray Diffraction (Xrd)supporting

confidence: 83%

Green Synthesis of Silver Nanoparticles and Testing its Effectiveness in Inhibiting E. coli, Staphylococcus Aureus Isolated from Urinary Tract Infections of Pregnant Women

Al-najar,

Hamad,

Abed

et al. 2024

JNRPSP

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The aim of the study was to identify the E. coli and S. aureus bacteria isolated from pregnant women with urinary tract infections that silver nanoparticles derived from green leaves inhibited. The silver nanoparticles showed inhibitory activity against these two types by Well diffusion method. towards bacteria. The result of the inhibition diameters by Well diffusion method were (35,33,30,25) for E.coli and (37,34,28,23) for S.aureus bacteria at concentrations (10,7.5,5,2.5)% respectively through the absence of colony growth on the surface of Muller Hunton agar plates at those concentrations. The use of nanoparticles as a result of their effective properties in inhibiting bacteria, provided that there are no side effects that affect its work. To make silver nanoparticles in an eco-friendly manner—that is, without heating the plant extract and solution or utilizing chemical catalysts during the reaction—one milliliter of strawberry leaf extract and fifty milliliters of silver nitrate solution—prepared at a concentration of one millimeter at room temperature for one to eight hours—were combined. Using UV spectrophotometers, scanning electron microscopy (SEM), XRD, and FTIR, it was determined that the preparation had begun when the mixture's color intensity increased after each of these eight hours had gone. The absorbance at 378 nm was visible in the UV-VIS spectrum. According to the results of scanning electron microscopy, the produced silver nanoparticles ranged in size from 37.35 nm. The biological method of producing silver nanoparticles in this low-cost and eco-friendly manner has shown promising results in preventing the growth of bacteria isolated from wound infections.

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Section: X-ray Diffraction (Xrd)supporting

confidence: 83%

Green Synthesis of Silver Nanoparticles and Testing its Effectiveness in Inhibiting E. coli, Staphylococcus Aureus Isolated from Urinary Tract Infections of Pregnant Women

Al-najar,

Hamad,

Abed

et al. 2024

JNRPSP

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“…All plates were incubated at 37°C for 18-48h. 42 The isolates were identified using classical biochemical methods by standard laboratory techniques. 43 Multidrug resistence (MDR) was characterised if a strain during in vitro antimicrobial susceptibility test (AST) showed resistance to three or more antimicrobial classes.…”

Section: Methodsmentioning

confidence: 99%

Coexistence of blaOXA-48, blaVIM, and blaSHV genes in Klebsiella pneumoniae and Escherichia coli isolated from urinary tract infections: Microbiological and epidemiological analysis

et al. 2023

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Objective: To investigate antimicrobial resistance mechanisms of isolated bacterial strains, and their correlation with virulence profile.Method: The cross-sectional study was conducted in January 2020 at outpatient health centres in Kafrelsheikh Governorate of Egypt, and comprised urine samples from patients regardless of age and gender. Midstream samples were collected into sterile swaps which were kept in ice-cooled boxes until transported to the laboratory within 5h. Antimicrobial resistance profile of the isolated Enterobacteriaceae was done using Kirby–Bauer disk diffusion method and was confirmed with Vitek compact 2. The phenotypic of carbapenemases and extended-spectrum beta lactamase was determined, and polymerase chain reaction was used, as appropriate. Data was analysed using SPSS 20.Results: Of the 199 patients, 101(50.7%) were females and 98(49.3%) were males. The majority 73(36.6%) were aged 30-50 years. Urinary tract infection was found in 68(34.2%) patients. In 28(41.2%) of these patients, there were 32 isolates of Enterobacterales; 21(65.62%) Klebsiella pneumoniae, 7(21.87%) Escherichia coli and 4(12.5%) Enterobacter cloacae. Of the 28(41.2%) patients, 24(85.7%) were infected with a single strain; 17(70.8%) Klebsiella pneumoniae, 4(16.7%) Escherichia coli and 3(12.5%) Enterobacter cloacae. In 3(10.7%) cases, there was co-infection with Escherichia coli and Klebsiella pneumoniae, and 1(3.6%) sample had mixed infection with Klebsiella pneumoniae and Enterobactercloacae. The other 40(58.8%) patients had other causative agents. Housewives, agricultural workers and those aged >50 years had a higher risk of urinary tract infections (p<0.05) Among Klebsiella pneumonia isolates, 6(28.5%) possessed carbapenemase-related genes and 4(19.1%) extended-spectrum beta lactamase-related genes. The carbapenemaserelated genes were bla-Verona integron-encoded metallo beta lactamase 6(100%) bla-New Delhi metallo beta lactamase-1 4(66.6%) and bla-oxacillinase-48 2(33.3%). The 4(19.1%) cases of extended-spectrum beta lactamaserelatedgenes had bla-temoneira gene 3(75%) and bla-sulfhydryl variable gene 4(100%). In Escherichia coli isolates, bla-oxacillinase-48 and bla-Cefotaximase genes were observed in 2(28.5%) cases. Virulence genes uridine diphosphateglucose 4-epimerase, fimbrial adhesion and mannose-resistance adhesin of Klebsiella spp genes in Klebsiella pneumoniae isolates were positive in in 16(76.2%), 14(66.7%) and 10(47.6%) cases, respectively. All 21(100%) isolates of Klebsiella pneumoniae were negative for mucoviscosity-associated gene A.Conclusion: There was evidence of the coexistence of bla- oxacillinase-48, bla-Verona integron-encoded metallo beta lactamase and bla-sulfhydryl variable genes in Klebsiella pneumoniae and Escherichia coli isolates from mixed urinary tract infection samples.Keywords: Carbapenemase, Klebsiella pneumoniae, Escherichia coli, Lactams

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Study of the Genomic Characterization of Antibiotic-Resistant Escherichia Coli Isolated From Iraqi Patients with Urinary Tract Infections

Mouhammed,

Gdoura

2023

Indian J Microbiol

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Microbiological Profile of Urinary Tract Infections with special Reference to Antibiotic Susceptibility Pattern of Escherichia coli Isolates

Cited by 7 publications

References 17 publications

Green Synthesis of Silver Nanoparticles and Testing its Effectiveness in Inhibiting E. coli, Staphylococcus Aureus Isolated from Urinary Tract Infections of Pregnant Women

Green Synthesis of Silver Nanoparticles and Testing its Effectiveness in Inhibiting E. coli, Staphylococcus Aureus Isolated from Urinary Tract Infections of Pregnant Women

Coexistence of blaOXA-48, blaVIM, and blaSHV genes in Klebsiella pneumoniae and Escherichia coli isolated from urinary tract infections: Microbiological and epidemiological analysis

Study of the Genomic Characterization of Antibiotic-Resistant Escherichia Coli Isolated From Iraqi Patients with Urinary Tract Infections

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