Microbiological contamination of conventional and reclaimed irrigation water: Evaluation and management measures

Rusiñol, Marta; Hundesa, Ayalkibet; Cárdenas-Youngs, Yexenia; Fernández-Bravo, Ana; Pérez-Cataluña, Alba; Moreno-Mesonero, Laura; Moreno, Yolanda; Calvo, Miquel; Alonso, J.L.; Figueras, María José; Araujo, Rosa; Bofill-Mas, Sı́lvia; Gironés, Rosina

doi:10.1016/j.scitotenv.2019.136298

Cited by 56 publications

(36 citation statements)

References 79 publications

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“…For the authors [26,27], the presence of this genus in the aquatic environment is natural because they are ubiquitous and especially present in the soil, on plants and in water. However, this genus can be involved in human and animal infections, especially in the contamination of fish [28].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Profile and Resistance Determination of Bacteria Isolated from Water in Southern Benin

Koudokpon

Dougnon

Lougbegnon

et al. 2021

JAMB

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Background: The environment plays an important role in the dissemination of multidrug resistant bacteria, especially through the aquatic ecosystem, including hospital effluents, rivers, but also spring water and drinking water. This study aims to determine selected antimicrobial resistance genes in some aquatic matrices in southern Benin. Methods: Collected water samples were filtered through a membrane 0.22 µm thick. After filtration, the membrane was deposited on Muëller Hinton agar. Then the colonies resulting from this subculture were subjected to a microbiological examination by the conventional method. The antibiotic sensitivity test was carried out by the Kirby Bauer method according to the recommendations of the French Society of Microbiology. Resistance genes were looked for by PCR. Results: Of the 222 water samples collected, 265 bacterial strains were isolated, the majority of which were strains of Coagulase Negative Staphylococcus (CNS) with 37.74% (n = 100), followed by strains of Klebsiella pneumoniae (21.89%; n = 58), Escherichia coli (10.57%; n = 28). All isolated gram-negative bacilli strains are multidrug resistant with resistance of all strains to amoxicillin, ampicillin and amoxicillin + clavulanic acid. Of the 15 resistance genes searched in the genome of Gram-negative bacilli strains, 8 were detected, namely the TEM, SHV, CTX-M15, VIM, NDM, SUL1, SUL2 and AADA genes. Resistance of CNS strains to amoxicillin, oxacillin and cefoxitin was observed. The meca gene was detected in all CNS strains. The vanA and VanB genes were only detected in strains isolated from drinking water in sachets collected from producers and street sellers. Conclusion: These results show the dissemination of resistance genes in Benin and once again confirms the urgency of a global fight against antimicrobial resistance.

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Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Profile and Resistance Determination of Bacteria Isolated from Water in Southern Benin

Koudokpon

Dougnon

Lougbegnon

et al. 2021

JAMB

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“…The presence of Aeromonas spp. in aquatic ecosystems was noted by numerous studies [25]. For the authors [26,27], the presence of this genus in the aquatic environment is natural because they are ubiquitous and especially present in the soil, on plants and in water.…”

Section: Discussionmentioning

confidence: 99%

Emergence of Multidrug-resistance Bacteria Isolated of Drinking Water, Groundwater and Hospital Wastewater in Southern Benin: Antibiotic Resistance Profile and Resistomes Determination

H¹,

Dougnon²,

Lougbegnon³

et al. 2021

Preprint

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Background: The environment plays an important role in the dissemination of multidrug resistant bacteria, especially through the aquatic ecosystem, which is made up of liquid hospital effluents, rivers, but also spring water and drinking water. This study aims to determinate resistomes in some aquatic matrices in southern Benin.Methods: These samples were filtered through a membrane 0.22 µm thick. After filtration, the membrane was deposited on Muëller Hinton agar. Then the colonies resulting from this subculture were subjected to a microbiological examination by the conventional method. The antibiotic sensitivity test was carried out by the Kirby Bauer method according to the recommendations of the French Society of Microbiology. Resistance genes were looked for by standard PCR and the PCR products were used for agarose gel electrophoresis.Results: Of the 222 water samples collected, 265 bacterial strains were isolated, the majority of which were strains of Coagulase Negative Staphylococcus (CNS) with 37.74% (n = 100), followed by strains of Klebsiella pneumoniae (21.89%; n = 58), Escherichia coli (10.57%; n = 28). All isolated gram-negative bacilli strains are multidrug resistant with resistance of all strains to amoxicillin, ampicillin and amoxicillin + clavulanic acid. Of the 15 resistance genes sought in the genome of Gram-negative bacilli strains, only 8 were detected, namely the TEM, SHV, CTX-M15, VIM, NDM, SUL1, SUL2 and AADA genes. Strong resistance of CNS strains to amoxicillin, oxacillin and cefoxitin was observed. The Mec A gene was detected in all CNS strains. While the Van A and Van B genes were only detected in strains isolated from drinking water in sachets collected from producers and street sellers.Conclusion: These results show the dissemination of resistance genes in the aquatic ecosystem of Benin and once again confirms the urgency of a global fight against antimicrobial resistance.

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“…The combined treatment resulted in a significant reduction in the levels of iron and fluoride (as per WHO standards) in comparison to the individual seed and leaf extracts of the Moringa oleifera plant. Another major concern associated with reduced water quality in developing countries is microbial contamination, which results in several waterborne diseases, such as diarrhea [ 48 , 49 ]. In this study, the seed and leaf extract of Moringa oleifera showed a remarkable reduction in the E. coli content of the water, thereby validating it as an effective natural coagulant in improving the water quality index.…”

Section: Discussionmentioning

confidence: 99%

Study to Investigate the Potential of Combined Extract of Leaves and Seeds of Moringa oleifera in Groundwater Purification

Alam

Pandey²,

Khan³

et al. 2020

IJERPH

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Several parts of the Moringa oleifera plant have revealed incredible potential for water quality improvement. However, the purification potential of a combined leaf and seed extract of Moringa oleifera plants remains unexplored. To the best of our knowledge, this research would be the first to work towards exploiting the combined potential of a leaf and seed extract of the Moringa oleifera plant in the process of water purification. In this study, we investigated the combined effectiveness of the leaf and seed extract in the purification of groundwater. The jar test method was used to analyze the effectiveness of Moringa plant extract (in combination) on different quality parameters of groundwater. Treatment with the combined plant extract (seed and leaf) resulted in significant improvement of various physicochemical (hardness, pH, turbidity, Total Dissolved Solid (TDS), and metallic impurities) and biological parameters (E.coli count) over individual seed and leaf extracts in groundwater samples. Experimental findings have strongly shown the enhanced purification efficacy of the hexane extract of combined plant materials in comparison to the individual extracts, thereby providing us with a potent natural coagulant that could combat the side effects of chemical coagulants.

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Microbiological contamination of conventional and reclaimed irrigation water: Evaluation and management measures

Cited by 56 publications

References 79 publications

Antibiotic Resistance Profile and Resistance Determination of Bacteria Isolated from Water in Southern Benin

Antibiotic Resistance Profile and Resistance Determination of Bacteria Isolated from Water in Southern Benin

Emergence of Multidrug-resistance Bacteria Isolated of Drinking Water, Groundwater and Hospital Wastewater in Southern Benin: Antibiotic Resistance Profile and Resistomes Determination

Study to Investigate the Potential of Combined Extract of Leaves and Seeds of Moringa oleifera in Groundwater Purification

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