Microbiological characterization and effect of resin composites in cervical lesions

Abstract

BackgroundNon carious cervical lesions associated to muscle hyperfunctions are increasing. Microhybrid resin composites are used to restore cervical abfractions. The purpose of this study was to investigate if resin composites modify tooth plaque, inducing an increment of cariogenic microflora and evaluate their effect, in vivo and in vitro, against S. mutans.Material and MethodsEight abfractions were restored with two microhybrid resin composites (Venus, Heraeus-Kulzer® and Esthet-X, Dentsply®), after gnatological therapy, in three patients with muscle hyperfunctions. For each abfraction three samples of plaque were taken from the cervical perimeter: before the restoration, one week and three months after restoration. The samples were evaluated both by traditional microbiological methods and by Polymerase Chain Reaction (PCR). In vitro, disk-shaped specimens of the two composites were prepared to estimate the effects against pre-cultured S. mutans, after incubation at 37°C for 24h and assessed by a turbidimetric technique.ResultsIn vivo no differences were found in plaque growth, for all samples, before and after restoration with both composites; in vitro, instead, a significant reduction of S. mutans growth was found between specimens of two composites (Mann-Whitney U-test p>0,06).ConclusionsIn this study a relevant consideration was elicited: composite materials, in vivo, do not modify plaque composition of non carious cervical lesions to a potential cariogenic plaque. Key words:Abfraction, restoration, S. mutans, composite, class V.

Influence of viscosity and chemical composition of composite resins in non-carious cervical restorations: 12-month randomized clinical trial

“…Non-carious cervical lesions (NCCLs) are usually described as the loss of dental structure at the cement-enamel junction that is not caused by dental caries [1]. This type of lesion is very common in the adult population [2].…”

Addition of substances to reduce the erosive potential of acidic beverages to tooth enamel: A scoping review

“…Clinically, reducing the erosive potential of a beverage implies decreasing the dissolution rate of dental tissues, even after the acidic challenges imposed by its ingestion, slowing up tooth aging 50 . Teeth affected by erosion suffer the loss of brightness and surface smoothness, with an advance occurring beyond the cervical region (appearing cavities or vitreous appearance of the dentin), especially in the posterior region 51 . In anterior teeth with thinner incisal edges, they may become prone to fractures 51 .…”

Cтратегії Спрямованого Впливу На Утворення Біоплівки Streptococcus Mutans Для Запобігання Карієсу Зубів

“…Експериментально доведено, що планктонні форми бактерій і грибів у більшості випадків спричиняють гострі запальні процеси і загострення хронічних захворювань (спостерігається при розриві біоплівки і дисемінації збудника), а мікроорганізми у стані стабільної біоплівкихронічні форми захворювань [17]. Слід зауважити, що якість відновлень дефектів твердих тканин зубів залежить не тільки від використаного пломбувального матеріалу, але і від індивідуальних особливостей пацієнта, таких як гігієна порожнини рота, низьковуглеводна дієта, буферна ємність слини і фактична відсутність Streptococcus mutans [18].…”