Microbiological Characteristics and Physiological Functionality of New Records of Yeasts from Wild Flowers in Yokjido, Korea

Hyun, S. K.; Lee, Jong-Soo

doi:10.5941/myco.2014.42.2.198

Cited by 8 publications

(3 citation statements)

References 21 publications

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“…The life cycle of yeast is complex and consists of cellular stages that include different shapes of cells (Knop 2011). Generally, the shape of yeast cells may vary from circular, spherical, oval, elliptical, elongated, rectangular, dumb-bell shaped to triangular (Hyun and Lee 2014;Dunkler et al 2021).…”

Section: Morphological Characteristics Of Yeast Scoby Coculturementioning

confidence: 99%

Improving SCOBY starter using co-culture of tapai and bakery yeast

2021

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Abstract. Urbahillah A, Jayus J, Nurhayati N. 2021. Improving SCOBY starter using co-culture of tapai and bakery yeast. Biodiversitas 22: 4617-4624. Kombucha is a beverage fermented by a symbiotic bacteria and yeast known as SCOBY (Symbiotic Culture of Bacteria and Yeast). Bacteria and yeast contribute to the formation of organic acids, aroma, taste, and flavor of kombucha. The commercial yeasts used in Indonesian are baker’s yeast and tapai yeast. This study was conducted to develop SCOBYco-culture with tapai yeast and baker’s yeast and evaluate its activity. The ingredients for the kombucha were cascara, water, and sugar, which were fermented with three formula starter, i.e. original SCOBY 10% w/v (SN), co-culture SCOBY 10% w/v with 0.1% w/v of baker’s yeast (SNR), and co-culture SCOBY 10% w/v with tapai yeast 0.1% w/v (SNT). The starter activity were determined based on the OD (Optical Density) value. Yeast screening was carried out on the dominant starter population. Furthermore, morphologically yeast was identified based on colony type, color, and shape of cell. Then yeast was identified by their fermentation profile using API 20C Aux Kit. Isolate A showed white colony with convex elevation and the cell was round-shaped. Colony of isolate B and isolate C were creamy in color and oval cell shaped. The API results revealed that the first isolate was identified as Candida famata, second isolate was as Candida krusei, and the third isolate was as Candida magnoliae. Three types of fungi were found from SCOBY, namely Mucor sp., Trichoderma sp., and Fusarium sp. Mucor sp. has non-septate hyphae, and round black spores. Trichoderma sp. has septate hyphae, greenish-white spores, and the conidia have the shape of globose to ellipsoidal Fusarium sp. has a mold with septate hyphae, yellowish-white colonies, and the conidia have the shape of obovoid. Bacteria, yeast, and mold present in the medium form a powerful symbiosis for produce metabolite.

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Section: Morphological Characteristics Of Yeast Scoby Coculturementioning

confidence: 99%

Improving SCOBY starter using co-culture of tapai and bakery yeast

2021

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“…Causes subcutaneous cryptococcosis (Serda Kantarcioğlu et al, 2007) and dermatitis in humans (Sugita et al, 2003) Petroleum contaminated environments (Yilmaz and Sayar, 2015;Yalçın et al, 2014) 4 Cryptococcus magnus isolated from leukemia patients (Khan et al, 2011) and in other human clinical samples (Ghajari et al, 2018) Isolated from grapes (Pantelides et al, 2015), plant leaves (Han et al, 2017) and from some cereals (Han et al, 2015) 5 Cryptococcus uzbekistanensis isolated from immune compromised patients (Powel et al, 2012) Plant flowers (Hyun and Lee, 2014), and known causative agents of canker in fruit trees (Dehghan-Niri et al, 2015). Also isolated from petroleum contaminated sites (Yalçın et al, 2014;Yılmaz et al, 2015) 6 Naganishia globosa Clinical samples (Pakshir et al, 2018) Soil (Kim et al, 2018), hyper saline soils (Mokhtarnejad et al, 2016) and lakes (Li et al, 2018) 7 Naganishia liquefaciens…”

Section: Potential Pathogenic Yeastmentioning

confidence: 99%

Previously unrecorded distribution of marine sediments derived yeast isolates revealed by DNA barcoding

PrasannaKumar

Sivasubramanian

Subramanian

et al. 2020

Preprint

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Marine ecosystems are least explored niches for yeast population and diversity. The present study aims to construct a comprehensive DNA barcode library for marine derived yeast species. As, we sequenced ITS gene for 1017 isolates belonging to 157 marine derived yeast species in 55 genera, 28 families, 14 orders, 8 classes of 2 Phyla (viz., Ascomycota and Basidiomycota) among which 13 yeast species were barcoded for the first time, we witnessed yeast species of both terrestrial and endemic marine origin. Difficulties were faced in taxonomic sequence validation due to large volume of sequencing trace files, variable length of sequences extracted and lack of reference sequences in public databases. Majority of the sequences (62.24%) were between 600 and 649 bps in length. K2P intra-species distance analysis conducted for selective groups yielded the average of 0.33% which was well within the previously proposed barcode gap value for yeast species (1.59%). ITS gene tree based identification conducted for selective species in Ascomycota and Basidomycota, precisely clustered the same species in single groups. About 60% of the yeast species recorded in the present study was previously unrecorded from the marine environment, among which 16.5% of yeast species were recognised human pathogens. Apart from releasing the barcode data in GenBank, provisions were made to access the entire dataset along with meta-data in the Barcode of life database under the project title “DNA barcoding marine yeast”. The present study constitutes the biggest dataset for the collection of marine yeast isolates and its DNA barcodes till date.

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“…Furthermore, we have studied the production of bioactive compounds from these wild yeasts. Recently, we screened for new yeast records from among yeasts found in Ulleungdo [ 8 ], Yokjido [ 9 ], and in orchards and an arboretum [ 10 ] and their mycological characteristics were investigated.…”

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confidence: 99%

Isolation and Identification of Yeasts from Wild Flowers Collected around Jangseong Lake in Jeollanam-do, Republic of Korea, and Characterization of the Unrecorded Yeast Bullera coprosmaensis

et al. 2015

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Several types of yeasts were isolated from wild flowers around Jangseong Lake in Jeollanam-do, Republic of Korea and identified by comparing the nucleotide sequences of the PCR amplicons for the D1/D2 variable domain of the 26S ribosomal DNA using Basic Local Alignment Search Tool (BLAST) analysis. In total, 60 strains from 18 species were isolated, and Pseudozyma spp. (27 strains), which included Pseudozyma rugulosa (7 strains) and Pseudozyma aphidis (6 strains), was dominant species. Among the 60 strains, Bullera coprosmaensis JS00600 represented a newly recorded yeast strain in Korea, and its microbiological characteristics were investigated. The yeast cell has an oval-shaped morphology measuring 1.4 × 1.7 µm in size. Bullera coprosmaensis JS00600 is an asporous yeast that exhibits no pseudomycelium formation. It grew well in vitamin-free medium as well as in yeast extract-malt extract broth and yeast extract-peptone-dextrose (YPD) broth, and it is halotolerant growing in 10% NaCl-containing YPD broth.

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Microbiological Characteristics and Physiological Functionality of New Records of Yeasts from Wild Flowers in Yokjido, Korea

Cited by 8 publications

References 21 publications

Improving SCOBY starter using co-culture of tapai and bakery yeast

Improving SCOBY starter using co-culture of tapai and bakery yeast

Previously unrecorded distribution of marine sediments derived yeast isolates revealed by DNA barcoding

Isolation and Identification of Yeasts from Wild Flowers Collected around Jangseong Lake in Jeollanam-do, Republic of Korea, and Characterization of the Unrecorded Yeast Bullera coprosmaensis

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