Microbiological and production characteristics of the high-temperature Kongdian petroleum reservoir revealed during field trial of biotechnology for the enhancement of oil recovery

Назина, Т. Н.; Aa, Grigor'ian; Ts, Feng; Nm, Shestakova; Бабич, Т. Л.; Nk, Pavlova; Vs, Ivoĭlov; F, Ni; Wang, J; She, Yuanbin; Ting-sheng, Xiang; Bai, Mei; Luo, Zhifeng; Ss, Beliaev; Mv, Ivanov

doi:10.1134/s002626170703006x

Cited by 22 publications

(14 citation statements)

References 10 publications

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“…Keeler, in preparation). Anthropogenic amendments of a petroleum reservoir have been shown to induce shifts in microbial populations and in situ biochemical activities (Nazina et al ., 2007), and a discrepancy between physicochemical conditions and microbial physiology (inferred through phylogenetic kinship) has been used to deduce the non‐indigenous nature of some petroleum microflora (Dahle et al ., 2008).…”

Section: Discussionmentioning

confidence: 99%

Characterizing microbial diversity in production water from an Alaskan mesothermic petroleum reservoir with two independent molecular methods

Pham

Hnatow

Zhang

et al. 2009

Environmental Microbiology

153

120

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The phylogenetic diversity of Bacteria and Archaea within a biodegraded, mesothermic petroleum reservoir in the Schrader Bluff Formation of Alaska was examined by two culture-independent methods based on fosmid and small-subunit rRNA gene PCR clone libraries. Despite the exclusion of certain groups by each method, there was overall no significant qualitative difference in the diversity of phylotypes recovered by the two methods. The resident Bacteria belonged to at least 14 phylum-level lineages, including the polyphyletic Firmicutes, which accounted for 36.2% of all small-subunit rRNA gene-containing (SSU(+)) fosmid clones identified. Members of uncultured divisions were also numerous and made up 35.2% of the SSU(+) fosmid clones. Clones from domain Archaea accounted for about half of all SSU(+) fosmids, suggesting that their cell numbers were comparable to those of the Bacteria in this microbial community. In contrast to the Bacteria, however, nearly all archaeal clones recovered by both methods were related to methanogens, especially acetoclastic methanogens, while the plurality of bacterial fosmid clones was affiliated with Synergistes-like acetogenic Firmicutes that possibly degrade longer-chain carboxylic acid components in the crude oil to acetate. These data suggest that acetate may be a key intermediary metabolite in this subsurface anaerobic food chain, which leads to methane production as the primary terminal electron sink.

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Section: Discussionmentioning

confidence: 99%

Characterizing microbial diversity in production water from an Alaskan mesothermic petroleum reservoir with two independent molecular methods

Pham

Hnatow

Zhang

et al. 2009

Environmental Microbiology

153

120

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“…It is interesting to find that although the same nutrients were injected in the oil reservoir, the stimulated bacteria appeared different in different production wells. In most previous field trials, MEOR processes detected the increase of survivable cells, the loss in substrates, and the appearance of specific products of metabolism (Nazina et al 2007a, b;Youssef et al 2007). Coupled with this study, difference in stimulated microbial populations between production wells has been perceived gradually with the monitoring of microbial communities by our research group (Zhang et al 2010).…”

Section: Differences In Stimulated Microbial Populations In Differentmentioning

confidence: 96%

Impact of an indigenous microbial enhanced oil recovery field trial on microbial community structure in a high pour-point oil reservoir

Zhang

She

et al. 2011

Appl Microbiol Biotechnol

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Based on preliminary investigation of microbial populations in a high pour-point oil reservoir, an indigenous microbial enhanced oil recovery (MEOR) field trial was carried out. The purpose of the study is to reveal the impact of the indigenous MEOR process on microbial community structure in the oil reservoir using 16Sr DNA clone library technique. The detailed monitoring results showed significant response of microbial communities during the field trial and large discrepancies of stimulated microorganisms in the laboratory and in the natural oil reservoir. More specifically, after nutrients injection, the original dominant populations of Petrobacter and Alishewanella in the production wells almost disappeared. The expected desirable population of Pseudomonas aeruginosa, determined by enrichment experiments in laboratory, was stimulated successfully in two wells of the five monitored wells. Unexpectedly, another potential population of Pseudomonas pseudoalcaligenes which were not detected in the enrichment culture in laboratory was stimulated in the other three monitored production wells. In this study, monitoring of microbial community displayed a comprehensive alteration of microbial populations during the field trial to remedy the deficiency of culture-dependent monitoring methods. The results would help to develop and apply more MEOR processes.

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“…Biosurfactants serve to decrease tension at the hydrocarbon-water interface and can result in hydrocarbon pseudosolubilization via micelle or vesicle formation, leading to increased mobility, bioavailability, and subsequent biodegradation [33,34]. Nazina et al [35] reported that biosurfactants were mostly produced by strains of Pseudomonas sp., Bacillus cereus, Rhodococcus ruber, and Bacillus licheniformis in media with hydrocarbons which could enhance the dissolved concentration of phenanthrene to above 1 mg/l, concentration higher than the saturated solubility of phenanthrene under standard condition. Carlos et al [36] has reported biosurfactant production by Bacillus cereus.…”

Section: Carbon Source A-2 a A-3 A A-4 A Consortium Amentioning

confidence: 99%

Biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment

Janbandhu

Fulekar

2011

Journal of Hazardous Materials

204

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Microbiological and production characteristics of the high-temperature Kongdian petroleum reservoir revealed during field trial of biotechnology for the enhancement of oil recovery

Cited by 22 publications

References 10 publications

Characterizing microbial diversity in production water from an Alaskan mesothermic petroleum reservoir with two independent molecular methods

Characterizing microbial diversity in production water from an Alaskan mesothermic petroleum reservoir with two independent molecular methods

Impact of an indigenous microbial enhanced oil recovery field trial on microbial community structure in a high pour-point oil reservoir

Biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment

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