Microbicidal activity measured by flow cytometry: Optimization and standardization for detection of primary and functional deficiencies

Jeraiby, Mohammed; Yahya, Kaddour; Berger, Anne‐Emmanuelle; Lambert, Claude

doi:10.1016/j.jim.2016.09.010

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…The main process that sustains the homeostasis of PMN is their oxidative activity. We have chosen to present only PMN’s oxidative activity as the other innate immune cells, eg, monocytes 21 have low levels of oxidative activity even upon stimulation, hence the overall oxidative activity is mainly sustained by PMNs.…”

Section: Resultsmentioning

confidence: 99%

Healthy Ageing Reflected in Innate and Adaptive Immune Parameters

Munteanu¹,

Surcel²,

Isvoranu³

et al. 2022

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Purpose The aim of the paper is to establish and quantify the relation between healthy ageing and the innate and adaptive immune parameters as indicators of age-related diseases. Patients In order to observe the immunological changes that occur according to age, several humoral and cellular immune parameters were investigated for 288 healthy donors (30–80 years). Subjects’ selection was done using clinical, biochemical and immunological parameters of inclusion/exclusion criteria from SENIEUR protocol. Results Age-related changes were observed for both humoral and cellular immune parameters. Lymphocyte immunophenotyping revealed several significant differences in the distribution of cells, both intra- and inter-age groups, namely decreased values of T-CD3 + , T-CD8 + and NK cells, and elevated values for T-CD4 + , T-CD4 + /T-CD8 + ratio and B cells. The percentages of unstimulated neutrophils that show basal oxidative activity and the intensity of this activity had an increasing tendency age-related. The percentage of N-Formyl-Methionyl-Leucyl-Phenylalanine stimulated neutrophils clearly decreases with age, and is associated with an increasing intensity of oxidative activity. Our data also have shown an increased percentage of oxidative neutrophils after phorbol 12-myristate 13-acetate stimulation and an elevated oxidative activity with age. Conclusion Overall healthy ageing is governed by some immune-related deregulations that account for immune exhaustion due to numerous developed immune processes during a life-time and the age-related diseases.

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Section: Resultsmentioning

confidence: 99%

Healthy Ageing Reflected in Innate and Adaptive Immune Parameters

Munteanu¹,

Surcel²,

Isvoranu³

et al. 2022

CIA

View full text Add to dashboard Cite

show abstract

“…Fluorescence is then directly proportional to the production of free radicles produced in individual cells, depending on the stimulation, as well as the cell proper capacity. More functional tests have been used and the procedures can be very simple and rapid, stimulating cells just before or even during the incubation time for immunolabelling [ 6 ].…”

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confidence: 99%