Microbially induced CaCO3 precipitation through denitrification: An optimization study in minimal nutrient environment

Erşan, Yusuf Çağatay; Belie, Nele De; Boon, Nico

doi:10.1016/j.bej.2015.05.006

Cited by 156 publications

(61 citation statements)

References 22 publications

(58 reference statements)

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“…In the presence of calcium ions, NO 3 -reduction induces CaCO 3 precipitation. Our research has revealed that through NO 3 -reduction, even enhanced CaCO 3 precipitation performances could be achieved in nutrient-poor environments which makes the mechanism feasible for selfhealing concrete [18,49]. Nitrate reduction can also lead to the intermediate production of NO 2 -which is known as corrosion inhibitor.…”

Section: Bacterial Self-healing To Increase Concrete 7 Durability Andmentioning

confidence: 88%

“…De Muynck et al [17] have shown that between various ureolytic strains even at lower temperatures of 10°C the mesophilic Bacillus sphaericus produced a higher amount of carbonate in the shortest amount of time than other more cold resistant strains like Sporosarcina psychrophila. Ersan et al [18] have investigated MICP through denitrification, in a minimal-nutrient environment. In this study, CaCO 3 precipitation performances of two newly isolated resilient strains Diaphorobacter nitroreducens and Pseudomonas aeruginosa were optimized and the repetitive CaCO 3 precipitation of a single inoculum was investigated.…”

Section: Selection Of Bacteriamentioning

confidence: 99%

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Application of bacteria in concrete: a critical evaluation of the current status

Belie

2016

RILEM Tech Lett

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Microbially induced carbonate precipitation has been tested over more than a decade as a technique to enhance concrete properties. Mainly bacteria following the pathways of urea decomposition, oxidation of organic acids, or nitrate reduction have been studied for this purpose. For bacteria mixed into fresh concrete, it is difficult to prove that they actively contribute to calcium carbonate precipitation and the effects on concrete strength are variable. Application of bacteria for surface consolidation has been shown to reduce water absorption and increase durability. Microbial self-healing of cracks in concrete shows promising results at the laboratory scale. Especially the use of self-protected mixed cultures opens perspectives for practical application. However, their self-healing efficiency needs to be further proven in larger concrete elements, and under non-ideal conditions. The use of denitrifying cultures for concurrent self-healing and production of corrosion inhibiting nitrites is a promising new strategy.

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Section: Bacterial Self-healing To Increase Concrete 7 Durability Andmentioning

confidence: 88%

Section: Selection Of Bacteriamentioning

confidence: 99%

Application of bacteria in concrete: a critical evaluation of the current status

Belie

2016

RILEM Tech Lett

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“…However, in the case of low oxygen concentration, such as for most underground structures, the efficiency of this approach can be limited. (3) The third pathway is known as dissimilatory nitrate reduction 16 . This approach is less efficient than the other two in terms of the amount of produced CaCO3, but it can be applied in anaerobic zones.…”

Section: Introductionmentioning

confidence: 99%

Screening of Fungi for Potential Application of Self-Healing Concrete

Menon

Luo

Chen

et al. 2019

Sci Rep

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Concrete is susceptible to cracking owing to drying shrinkage, freeze-thaw cycles, delayed ettringite formation, reinforcement corrosion, creep and fatigue, etc. Continuous inspection and maintenance of concrete infrastructure require onerous labor and high costs. If the damaging cracks can heal by themselves without any human interference or intervention, that could be of great attraction. In this study, a novel self-healing approach is investigated, in which fungi are applied to heal cracks in concrete by promoting calcium carbonate precipitation. The goal of this investigation is to discover the most appropriate species of fungi for the application of biogenic crack repair. Our results showed that, despite the significant pH increase owing to the leaching of calcium hydroxide from concrete, Aspergillus nidulans (MAD1445), a pH regulatory mutant, could grow on concrete plates and promote calcium carbonate precipitation.

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“…However, this approach requires high concentrations of calcium source 29 , which could possibly lead to buidup of high level of salts in concrete. The third pathway to precipitate CaCO3 is known as dissimilatory nitrate reduction 23 . Mineral production is promoted through oxidation of organic compounds through nitrate reduction by means of denitrifying bacteria.…”

Section: Introductionmentioning

confidence: 99%

Interactions of fungi with concrete: Significant importance for bio-based self-healing concrete

Luo

Chen

Crump

et al. 2018

Construction and Building Materials

125

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The goal of this study is to explore a new self-healing concept in which fungi are used as a self-healing agent to promote calcium mineral precipitation to fill the cracks in concrete. An initial screening of different species of fungi has been conducted. Fungal growth medium was overlaid onto cured concrete plate. Mycelial discs were aseptically deposited at the plate center. The results showed that, due to the dissolving of Ca(OH)2 from concrete, the pH of the growth medium increased from its original value of 6.5 to 13.0. Despite the drastic pH increase, Trichoderma reesei (ATCC13631) spores germinated into hyphal mycelium and grew equally well with or without concrete. X-ray diffraction (XRD) and scanning electron microscope (SEM) confirmed that the crystals precipitated on the fungal hyphae were composed of calcite. These results indicate that T. reesei has great potential to be used in bio-based self-healing concrete for sustainable infrastructure.

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Microbially induced CaCO3 precipitation through denitrification: An optimization study in minimal nutrient environment

Cited by 156 publications

References 22 publications

Application of bacteria in concrete: a critical evaluation of the current status

Application of bacteria in concrete: a critical evaluation of the current status

Screening of Fungi for Potential Application of Self-Healing Concrete

Interactions of fungi with concrete: Significant importance for bio-based self-healing concrete

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