Microbial xylanases in xylooligosaccharide production from lignocellulosic feedstocks

Yeğin, Sırma

doi:10.1007/s13399-022-03190-w

Cited by 11 publications

(9 citation statements)

References 163 publications

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“…In both strategies, the process starts with a pretreatment step to solubilize the xylan from the hemicellulose. Considering that XOS with lower DPs have higher functional properties, and that hemicellulose is the fraction of biomass which solubilizes most easily [28], in single-step processes the severity of pretreatment necessary to achieve high biomass solubilization Considering that XOS with lower DPs have higher functional properties, and that hemicellulose is the fraction of biomass which solubilizes most easily [28], in single-step processes the severity of pretreatment necessary to achieve high biomass solubilization yields can lead to a high concentration of monomers [27], which is not desirable because this will impair the purification stage, as will be described in the next sections. In this sense, most of the reports use the two-step strategy, where the pretreatment is followed by enzymatic hydrolysis of the biomass [29].…”

Section: Xos Productionmentioning

confidence: 99%

“…Additionally, the design of the process is not trivial, since it depends on many factors, such as structural complexity, diverse composition, seasonality, and risk of deterioration [ 3 , 8 ]. There are different technologies available, but in general they can be classified as single-stage or two-stage approaches ( Figure 5 ) [ 27 ]. In both strategies, the process starts with a pretreatment step to solubilize the xylan from the hemicellulose.…”

Section: Xos Productionmentioning

confidence: 99%

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Xylooligosaccharides: A Bibliometric Analysis and Current Advances of This Bioactive Food Chemical as a Potential Product in Biorefineries’ Portfolios

Manicardi

Silva

Longati

et al. 2023

Foods

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Xylooligosaccharides (XOS) are nondigestible compounds of great interest for food and pharmaceutical industries due to their beneficial prebiotic, antibacterial, antioxidant, and antitumor properties. The market size of XOS is increasing significantly, which makes its production from lignocellulosic biomass an interesting approach to the valorization of the hemicellulose fraction of biomass, which is currently underused. This review comprehensively discusses XOS production from lignocellulosic biomass, aiming at its application in integrated biorefineries. A bibliometric analysis is carried out highlighting the main players in the field. XOS production yields after different biomass pretreatment methods are critically discussed using Microsoft PowerBI® (2.92.706.0) software, which involves screening important trends for decision-making. Enzymatic hydrolysis and the major XOS purification strategies are also explored. Finally, the integration of XOS production into biorefineries, with special attention to economic and environmental aspects, is assessed, providing important information for the implementation of biorefineries containing XOS in their portfolio.

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Section: Xos Productionmentioning

confidence: 99%

Section: Xos Productionmentioning

confidence: 99%

Xylooligosaccharides: A Bibliometric Analysis and Current Advances of This Bioactive Food Chemical as a Potential Product in Biorefineries’ Portfolios

Manicardi

Silva

Longati

et al. 2023

Foods

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“…Xylooligosaccharides (XOSs) are oligomers composed of β-D-xylopyranosyl (xylose) units with β-(1,4)-xylosidic linkages, with a degree of polymerization of 2–7 ( Kaur et al, 2023 ). Prebiotic properties of XOSs, including reduced blood cholesterol and colon cancer risk; increased calcium absorption, antioxidant effects, and intestinal functions; and the medical benefit of cytotoxic activity on human leukemia cells ( Amorim et al, 2019 ; Yegin, 2022 ). XOSs also benefit patients with type two diabetes mellitus by acting as substitute sweeteners without increasing blood sugar ( Yan et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Gene cloning, expression, and characterization of two endo-xylanases from Bacillus velezensis and Streptomyces rochei, and their application in xylooligosaccharide production

Zhang,

Qin,

Wang

et al. 2023

Front. Microbiol.

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Endo-xylanase hydrolyzing xylan in cellulosic residues releasing xylobiose as the major product at neutral pH are desirable in the substitute sweeteners industry. In this study, two endo-xylanases were obtained from Streptomyces rochei and Bacillus velezensis. SrocXyn10 showed the highest identity of 77.22%, with a reported endo-xylanase. The optimum reaction temperature and pH of rSrocXyn10-Ec were pH 7.0 and 60°C, with remarkable stability at 45°C or pHs ranging from 4.5 to 11.0. rBvelXyn11-Ec was most active at pH 6.0 and 50°C, and was stable at 35°C or pH 3.5 to 10.5. Both rSrocXyn10-Ec and rBvelXyn11-Ec showed specific enzyme activities on wheat arabinoxylan (685.83 ± 13.82 and 2809.89 ± 21.26 U/mg, respectively), with no enzyme activity on non-xylan substrates. The Vmax of rSrocXyn10-Ec and rBvelXyn11-Ec were 467.86 U mg−1 and 3067.68 U mg−1, respectively. The determined Km values of rSrocXyn10-Ec and rBvelXyn11-Ec were 3.08 g L−1 and 1.45 g L−1, respectively. The predominant product of the hydrolysis of alkaline extracts from bagasse, corncob, and bamboo by rSrocXyn10-Ec and rBvelXyn11-Ec were xylooligosaccharides. Interestingly, the xylobiose content in hydrolysates by rSrocXyn10-Ec was approximately 80%, which is higher than most reported endo-xylanases. rSrocXyn10-Ec and rBvelXyn11-Ec could be excellent candidates to produce xylooligosaccharides at neutral/near-neutral pHs. rSrocXyn10-Ec also has potential value in the production of xylobiose as a substitute sweetener.

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“…Moreover, enzymatic degradation has high specificity and minimal production of undesirable byproducts. The enzymatic production of XOS from xylan involves various enzymes, including endo-1,4-β-xylanases, β-xylosidase, α-glucosiduronase, α- l -arabinofuranosidase, acetyl xylan esterase, ferulic acid esterase, and ρ-coumaric acid esterase [ 12 ]. Usually, the term “xylanase” refers to endo-1,4-β-xylanases, which are the key enzyme to produce XOS from xylan by hydrolyzing the 1,4-β xylose linkages in the xylan backbone, resulting in the generation of different XOS and small amounts of xylose [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Currently, xylanases have been isolated from various sources such as bacteria, archaea, fungi, and insects. Generally, microbial xylanases are known for their high thermal stability and alkaline tolerance properties [ 12 , 13 ]. For example, an endo-xylanase derived from Aspergillus niger MTCC 9687 with an optimal temperature of 43.5 ℃ and optimal pH of 5.5, was used to hydrolyze alkali-pretreated cauliflower stalk, resulting in the production of xylobiose as the major XOS [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Discovery of novel alkaline-tolerant xylanases from fecal microbiota of dairy cows

Zhang,

Miao,

Tang

et al. 2023

Biotechnol Biofuels

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Xylo-oligosaccharides (XOS) are considered as a promising type of prebiotics that can be used in foods, feeds, and healthcare products. Xylanases play a key role in the production of XOS from xylan. In this study, we conducted a metagenomic analysis of the fecal microbiota from dairy cows fed with different types of fodders. Despite the diversity in their diets, the main phyla observed in all fecal microbiota were Firmicutes and Bacteroidetes. At the genus level, one group of dairy cows that were fed probiotic fermented herbal mixture-containing fodders displayed decreased abundance of Methanobrevibacter and increased growth of beneficial Akkermansia bacteria. Additionally, this group exhibited a high microbial richness and diversity. Through our analysis, we obtained a comprehensive dataset comprising over 280,000 carbohydrate-active enzyme genes. Among these, we identified a total of 163 potential xylanase genes and subsequently expressed 34 of them in Escherichia coli. Out of the 34 expressed genes, two alkaline xylanases with excellent temperature stability and pH tolerance were obtained. Notably, CDW-xyl-8 exhibited xylanase activity of 96.1 ± 7.5 U/mg protein, with an optimal working temperature of 55 ℃ and optimal pH of 8.0. CDW-xyl-16 displayed an activity of 427.3 ± 9.1 U/mg protein with an optimal pH of 8.5 and an optimal temperature at 40 ℃. Bioinformatic analyses and structural modeling suggest that CDW-xyl-8 belongs to GH10 family xylanase, and CDW-xyl-16 is a GH11 family xylanase. Both enzymes have the ability to hydrolyze beechwood xylan and produce XOS. In conclusion, this metagenomic study provides valuable insights into the fecal microbiota composition of dairy cows fed different fodder types, revealing main microbial groups and demonstrating the abundance of xylanases. Furthermore, the characterization of two novel xylanases highlights their potential application in XOS production.

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Microbial xylanases in xylooligosaccharide production from lignocellulosic feedstocks

Cited by 11 publications

References 163 publications

Xylooligosaccharides: A Bibliometric Analysis and Current Advances of This Bioactive Food Chemical as a Potential Product in Biorefineries’ Portfolios

Xylooligosaccharides: A Bibliometric Analysis and Current Advances of This Bioactive Food Chemical as a Potential Product in Biorefineries’ Portfolios

Gene cloning, expression, and characterization of two endo-xylanases from Bacillus velezensis and Streptomyces rochei, and their application in xylooligosaccharide production

Discovery of novel alkaline-tolerant xylanases from fecal microbiota of dairy cows

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