Microbial Utilization of Glycerol for Biomanufacturing

Keita, Valeriane Malika; Gonzalez‐Villanueva, Miriam; Wong, Tuck Seng; Tee, Kang Lan

doi:10.1007/978-981-15-2604-6_16

Cited by 3 publications

(1 citation statement)

References 184 publications

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“…This particular adaptation was guided by two key considerations. Firstly, glycerol stands as an appealing feedstock for biomanufacturing purposes [21][22]. Secondly, while ALE has been applied to enhance glycerol utilization in various microbes, it has typically been carried out at lower glycerol concentrations.…”

Section: Divalent Metal Ions Altered the Growth Performance Of Microb...mentioning

confidence: 99%

Fast-track adaptive laboratory evolution ofCupriavidus necatorH16 with divalent metal cations

Sitompul,

Price,

Tee

et al. 2023

Preprint

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Microbial strain improvement through adaptive laboratory evolution (ALE) has been a key strategy in biotechnology for enhancing desired phenotypic traits. Here, we present an accelerated ALE (aALE) workflow and its successful implementation in evolving C. necator H16 for enhanced tolerance towards elevated glycerol concentrations. The method involves the deliberate induction of genetic diversity through controlled exposure to divalent metal cations, enabling the rapid identification of improved variants. Through this approach, we observed the emergence of robust variants capable of growing in high glycerol concentration environments, demonstrating the efficacy of our aALE workflow. Our method offers several advantages over traditional ALE approaches, including its independence from genetically modified strains, specialized genetic tools, and potentially hazardous DNA-modifying agents. By utilizing divalent metal cations as mutagens, we offer a safer, more efficient, and cost-effective alternative for expansion of genetic diversity. With its ability to foster rapid microbial evolution, aALE serves as a valuable addition to the ALE toolbox, holding significant promise for the advancement of microbial strain engineering and bioprocess optimization.

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Section: Divalent Metal Ions Altered the Growth Performance Of Microb...mentioning

confidence: 99%

Fast-track adaptive laboratory evolution ofCupriavidus necatorH16 with divalent metal cations

Sitompul,

Price,

Tee

et al. 2023

Preprint

Self Cite

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A new conceptual process design and economic analysis of a fatty acids and glycerine production plant using palm oil

Foo

Kim

et al. 2022

Chem. Pap.

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Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock

Keita,

Lee,

Lakshmanan

et al. 2024

Microb Cell Fact

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Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. Graphical abstract

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Microbial Utilization of Glycerol for Biomanufacturing

Cited by 3 publications

References 184 publications

Fast-track adaptive laboratory evolution ofCupriavidus necatorH16 with divalent metal cations

Fast-track adaptive laboratory evolution ofCupriavidus necatorH16 with divalent metal cations

A new conceptual process design and economic analysis of a fatty acids and glycerine production plant using palm oil

Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock

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