Microbial transformations of fossil organic matter of Kupferschiefer black shale – elements mobilization from metalloorganic compounds and metalloporphyrins by a community of indigenous microorganisms

Matlakowska, Renata; Ruszkowski, Dariusz; Skłodowska, Aleksandra

doi:10.5277/ppmp130120

Cited by 4 publications

(1 citation statement)

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“…As part of the research, we compared: (1) the content of CoPPIX; (2) the content of heme b and heme iron; (3) the presence of hemoproteins such as cytochromes, peroxidase, and catalase; and (4) the presence of enzymes associated with heme biosynthetic pathway; proteins of heme uptake and sirohydrochlorin cobaltochelatase/sirohydrochlorin cobalt-lyase in the metaproteome of BISR and the proteome of the LM27 strain grown on two types of media (SR-BC, CoPPIX-BC). We chose CoPPIX for laboratory tests because literature data suggest that in the studied shale rock, protoporphyrins are present only in complexes with metals, including cobalt [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Sedimentary Cobalt Protoporphyrin as a Potential Precursor of Prosthetic Heme Group for Bacteria Inhabiting Fossil Organic Matter-Rich Shale Rock

Stasiuk

Matlakowska

2021

Biomolecules

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This study hypothesizes that bacteria inhabiting shale rock affect the content of the sedimentary cobalt protoporphyrin present in it and can use it as a precursor for heme synthesis. To verify this hypothesis, we conducted qualitative and quantitative comparative analyses of cobalt protoporphyrin as well as heme, and heme iron in shale rock that were (i) inhabited by bacteria in the field, (ii) treated with bacteria in the laboratory, and with (iii) bacterial culture on synthetic cobalt protoporphyrin. Additionally, we examined the above-mentioned samples for the presence of enzymes involved in the heme biosynthesis and uptake as well as hemoproteins. We found depletion of cobalt protoporphyrin and a much higher heme concentration in the shale rock inhabited by bacteria in the field as well as the shale rock treated with bacteria in the laboratory. Similarly, we observed the accumulation of protoporphyrin in bacterial cells grown on synthetic cobalt protoporphyrin. We detected numerous hemoproteins in metaproteome of bacteria inhabited shale rock in the field and in proteomes of bacteria inhabited shale rock and synthetic cobalt protoporhyrin in the laboratory, but none of them had all the enzymes involved in the heme biosynthesis. However, proteins responsible for heme uptake, ferrochelatase and sirohydrochlorin cobaltochelatase/sirohydrochlorin cobalt-lyase were detected in all studied samples.

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Section: Introductionmentioning

confidence: 99%