Microbial transformation of water-insoluble substrates by two types of novel interface bioprocesses, tacky liquid–liquid interface bioreactor and non-aqueous sporular bioconversion system

Oda, Shinobu

doi:10.1007/s11274-020-02834-1

Cited by 9 publications

(2 citation statements)

References 69 publications

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“…Liquid-liquid or solid-liquid two-phase systems may offer solutions, as they separate the substrate and product from each other or from the producer cells. This applies not only to vegetative cells but also to fungal spores [22]. The author emphasised that spore cells should not be confused with resting cells because they are metabolically active and that spores are often solventtolerant enough to survive in the organic phase, which opens intriguing bioprocess options.…”

Section: Progress In Fermentation Processesmentioning

confidence: 99%

Improved Foods Using Enzymes from Basidiomycetes

Berger

Ersoy

2022

Processes

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Within the kingdom of fungi, the division Basidiomycota represents more than 30,000 species, some with huge genomes indicating great metabolic potential. The fruiting bodies of many basidiomycetes are appreciated as food (“mushrooms”). Solid-state and submerged cultivation processes have been established for many species. Specifically, xylophilic fungi secrete numerous enzymes but also form smaller metabolites along unique pathways; both groups of compounds may be of interest to the food processing industry. To stimulate further research and not aim at comprehensiveness in the broad field, this review describes some recent progress in fermentation processes and the knowledge of fungal genetics. Processes with potential for food applications based on lipases, esterases, glycosidases, peptidases and oxidoreductases are presented. The formation and degradation of colourants, the degradation of harmful food components, the formation of food ingredients and particularly of volatile and non-volatile flavours serve as examples. In summary, edible basidiomycetes are foods—and catalysts—for food applications and rich donors of genes to construct heterologous cell factories for fermentation processes. Options arise to support the worldwide trend toward greener, more eco-friendly and sustainable processes.

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Section: Progress In Fermentation Processesmentioning

confidence: 99%

Improved Foods Using Enzymes from Basidiomycetes

Berger

Ersoy

2022

Processes

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“…The low water solubility and mass transfer of hydrophobic synthetic substrates limit the efficiency of biocatalytic process (Oda 2020 ; Pollard et al 2006 ; Tang et al 2020 ). These limitations can be alleviated to a certain extent by introducing proper surfactant into the reaction medium.…”

Section: Introductionmentioning

confidence: 99%

Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution

et al. 2021

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Abstract(R)-1-[3-(Trifluoromethyl)phenyl]ethanol ((R)-MTF-PEL) is an important chiral building block for the synthesis of a neuroprotective compound, (R)-3-(1-(3-(trifluoromethyl)phenyl)ethoxy)azetidine-1-carboxamide. In this work, an effective whole-cell-catalyzed biotransformation was developed to produce (R)-MTF-PEL, and its productivity was increased by medium engineering strategy. The recombinant E. coli BL21(DE3)-pET28a(+)-LXCAR-S154Y variant affording carbonyl reductase was adopted for the reduction of 3'-(trifluoromethyl)acetophenone to (R)-MTF-PEL with enantiomeric excess (ee) > 99.9%. The addition of 0.6% Tween-20 (w/v) boosted the bioreduction, because the substrate concentration was increased by 4.0-fold than that in the neat buffer solution. The biocatalytic efficiency was further enhanced by introducing choline chloride: lysine (ChCl:Lys, molar ratio of 1:1) in the reaction medium, because the product yield reached 91.5% under 200 mM substrate concentration in the established Tween-20/ChCl:Lys-containing system, which is the highest ever reported for (R)-MTF-PEL production. The optimal reduction conditions were as follows: 4% (w/v) ChCl:Lys, 12.6 g (DCW)/L recombinant E. coli cells, pH 7.0, 30 ℃ and 200 rpm, reaction for 18 h. The combined strategy of surfactant and NADES has great potential in the biocatalytic process and the synthesis of chiral alcohols.

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Non-catalytic and Catalytic Co-pyrolysis of Lignocellulosic-Lignocellulosic Waste

Poddar

Babu

2022

Advances in Chemical, Bio and Environmental Engineering

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Microbial transformation of water-insoluble substrates by two types of novel interface bioprocesses, tacky liquid–liquid interface bioreactor and non-aqueous sporular bioconversion system

Cited by 9 publications

References 69 publications

Improved Foods Using Enzymes from Basidiomycetes

Improved Foods Using Enzymes from Basidiomycetes

Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution

Non-catalytic and Catalytic Co-pyrolysis of Lignocellulosic-Lignocellulosic Waste

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