Microbial synthesized cadmium oxide nanoparticles induce oxidative stress and protein leakage in bacterial cells

Azam, Zara; Ayaz, Asma; Younas, Mohammad; Qureshi, Zeeshan; Arshad, Bushra; Zaman, Wajid; Ullah, Fazal; Nasar, Muhammad; Bahadur, Saraj; Irfan, Mian Muhammad; Hussain, Sajjad; Saqib, Saddam

doi:10.1016/j.micpath.2020.104188

Cited by 67 publications

(31 citation statements)

References 47 publications

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“…The FTIR analysis of the chemically synthesized AgNPs revealed that methyl, carboxyl, Si–OR, COO − N–H groups were attached with AgNPs (Figure 4a,b). The use of FTIR spectroscopic techniques for the nondestructive analysis of biological specimens is a rapidly expanding research area (Azam et al, 2020; Ishtiaq et al, 2020; Rubab et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Comparative evaluation of the toxicological effect of silver salt (AgNO₃) and silver nanoparticles on Cyprinus carpio synthesized by chemicals and marine algae using scanning electron microscopy

Liaqat

Hanif

Bahadur

et al. 2021

Microscopy Res & Technique

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The widespread use of silver nanoparticles (AgNPs) results in the unintentional release into the water body. Therefore, understanding of the potentially harmful impacts of AgNPs and Ag‐salt on aquatic animals is a need of time. This study was design to analyze the oxidative stress and histopathological damages in Cyprinus carpio. The synthesis of AgNPs from Halymenia porphyraeformis and by reduction of chemical was done. Nanoparticles were characterized with UV–Visible spectroscopy, SEM, XRD, and FTIR analysis. The comparative toxicological effect of chemically synthesized silver nanoparticles (Ch‐AgNPs), green silver nanoparticles (Gr‐AgNPs), and Ag‐salt on C. carpio was analyzed. For oxidative stress analysis, different tests Lipid peroxidation (LPO), catalase, glutathione reduction (GST), and glutathione S‐transferase (GST) were performed. The highest LPO 245.168 ± 0.034 was recorded in Ch‐AgNPs‐treated gills and the lowest 56.4532 ± 0.02 was found in Gr‐AgNPs‐treated liver. Maximum GSH 56.4065 ± 0.13 was observed in Gr‐AgNPs liver and minimum 40.781 ± 0.54 was recorded in Ag‐salt gills. The maximum quantity of catalase 68.0162 ± 0.09 was noted in the Ag‐salt‐treated liver and the minimum was calculated 17.3665 ± 0.01 in the liver of Ch‐AgNPs and highest values of GST 765.829 ± 0.11 were recorded in gills of Gr‐AgNPs and lowest 633.08 ± 0.26 in the liver of Ch‐AgNPs‐treated fish. In conclusion, maximum destruction was found in the gills and liver of the fish treated with chemical and green AgNPs followed by Ag‐salt as compared to control. The adverse effects of AgNPs and Ag‐salt were probably related to the oxidative stress in the fish that lead to histopathological damage of its vital organs.

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Section: Resultsmentioning

confidence: 99%

Comparative evaluation of the toxicological effect of silver salt (AgNO₃) and silver nanoparticles on Cyprinus carpio synthesized by chemicals and marine algae using scanning electron microscopy

Liaqat

Hanif

Bahadur

et al. 2021

Microscopy Res & Technique

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“…ZnO NPs produce H 2 O 2 and hydroxyl radicals (OH − ), but not superoxide (

) and have weak mutagenic capability that induces frameshift mutation in bacterium [ 36 , 37 , 38 ]. Metal NPs such as AgNPs have an oligodynamic effect (the biocidal effects of metals) due to their large surface areas and have the ability to accumulate at the cell wall and bind with bacterial biomolecules [ 39 ] and penetrate the cells [ 40 ], generating ROS and free radicals, and act as modulators in the signal transduction pathways of microorganisms [ 41 , 42 , 43 ].…”

Section: Introductionmentioning

confidence: 99%

A Machine Learning Tool to Predict the Antibacterial Capacity of Nanoparticles

et al. 2021

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The emergence and rapid spread of multidrug-resistant bacteria strains are a public health concern. This emergence is caused by the overuse and misuse of antibiotics leading to the evolution of antibiotic-resistant strains. Nanoparticles (NPs) are objects with all three external dimensions in the nanoscale that varies from 1 to 100 nm. Research on NPs with enhanced antimicrobial activity as alternatives to antibiotics has grown due to the increased incidence of nosocomial and community acquired infections caused by pathogens. Machine learning (ML) tools have been used in the field of nanoinformatics with promising results. As a consequence of evident achievements on a wide range of predictive tasks, ML techniques are attracting significant interest across a variety of stakeholders. In this article, we present an ML tool that successfully predicts the antibacterial capacity of NPs while the model’s validation demonstrates encouraging results (R2 = 0.78). The data were compiled after a literature review of 60 articles and consist of key physico-chemical (p-chem) properties and experimental conditions (exposure variables and bacterial clustering) from in vitro studies. Following data homogenization and pre-processing, we trained various regression algorithms and we validated them using diverse performance metrics. Finally, an important attribute evaluation, which ranks the attributes that are most important in predicting the outcome, was performed. The attribute importance revealed that NP core size, the exposure dose, and the species of bacterium are key variables in predicting the antibacterial effect of NPs. This tool assists various stakeholders and scientists in predicting the antibacterial effects of NPs based on their p-chem properties and diverse exposure settings. This concept also aids the safe-by-design paradigm by incorporating functionality tools.

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“…Sculpturing of Exine was observed as reticulate, microreticulate, and bireticulate, with colpus surface ornamentation verrucate, gemmate, scabrate, and psilate. These species further recommended for medicinal and pharmacological potential (Ashfaq et al, 2019; Bahadur et al, 2020; Zaman et al, 2020), macromorphological (Khan, Ahmad, Shuaib, Bahadur, & Hussain, 2020), nanoparticles (Azam, Ayaz, et al, 2020; Azam, Khan, et al, 2020), pharmacological, and antimicrobial potential (Rubab et al, 2020), ecological significance (Sajad, Khan, Bahadur, Naeem, et al, 2020; Sajad, Khan, Bahadur, Shuaib, et al, 2020), its association with other plants (Azam, Ayaz, et al, 2020; Azam, Khan, et al, 2020; Naeem et al, 2020; Sarah et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Palynological characteristics of selected Lamioideae taxa and its taxonomic significance

Gul

Ahmad

Zafar

et al. 2020

Microscopy Res & Technique

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Lamioideae comprised the second‐largest subfamily in Lamiaceae. Although considerable progress has recently been made in the taxonomic study of Lamioideae, the subfamily remains one of the most poorly investigated subfamily in Lamiaceae. Therefore, the present study was designed with the aim to document the pollen micromorphology of some selected Lamioideae taxa and its taxonomic significance from Pakistan. Pollen micromorphological features were observed using scanning electron microscopy. The pollen grains are monad, tricolpate, radially/bilateral symmetrical. The pollen grains were small to medium‐sized having oblate, oblate/subspheroidal, and subspheroidal shape. Exine sculpturing was observed as reticulate, microreticulate, and bireticulate. The colpus surface ornamentation was found as verrucate, gemmate, scabrate, and psilate. There was a considerable variation between the species in the micromorphology, that is, the coarseness of the reticulum, thickness of the muri comprising the reticulum and the number of secondary lumina per primary lumen. Hence, this study documented the pollen morphology of some selected taxa of the subfamily Lamioideae from Pakistan and strengthens the taxonomic identification of subfamily based on pollen characters, which helps in the correct identification, discrimination of the species of Lamioideae at generic and species level.

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Microbial synthesized cadmium oxide nanoparticles induce oxidative stress and protein leakage in bacterial cells

Cited by 67 publications

References 47 publications

Comparative evaluation of the toxicological effect of silver salt (AgNO₃) and silver nanoparticles on Cyprinus carpio synthesized by chemicals and marine algae using scanning electron microscopy

Comparative evaluation of the toxicological effect of silver salt (AgNO₃) and silver nanoparticles on Cyprinus carpio synthesized by chemicals and marine algae using scanning electron microscopy

A Machine Learning Tool to Predict the Antibacterial Capacity of Nanoparticles

Palynological characteristics of selected Lamioideae taxa and its taxonomic significance

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Microbial synthesized cadmium oxide nanoparticles induce oxidative stress and protein leakage in bacterial cells

Cited by 67 publications

References 47 publications

Comparative evaluation of the toxicological effect of silver salt (AgNO3) and silver nanoparticles on Cyprinus carpio synthesized by chemicals and marine algae using scanning electron microscopy

Comparative evaluation of the toxicological effect of silver salt (AgNO3) and silver nanoparticles on Cyprinus carpio synthesized by chemicals and marine algae using scanning electron microscopy

A Machine Learning Tool to Predict the Antibacterial Capacity of Nanoparticles

Palynological characteristics of selected Lamioideae taxa and its taxonomic significance

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Product

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Comparative evaluation of the toxicological effect of silver salt (AgNO₃) and silver nanoparticles on Cyprinus carpio synthesized by chemicals and marine algae using scanning electron microscopy

Comparative evaluation of the toxicological effect of silver salt (AgNO₃) and silver nanoparticles on Cyprinus carpio synthesized by chemicals and marine algae using scanning electron microscopy