MICROBIAL SYNTHESIS OF RHAMNOLIPIDS BYPseudomonas aeruginosa(ATCC 10145) ON WASTE FRYING OIL AS LOW COST CARBON SOURCE

Abstract

Vegetable edible oils and fats are mainly used for frying purposes in households and the food industry. The oil undergoes degradation during frying and hence has to be replaced from time to time. Rhamnolipids are produced by microbial cultivation using refined vegetable oils as a carbon source and Pseudomonas aeruginosa (ATCC 10145). The raw material cost accounts for 10-30% of the overall cost of biosurfactant production and can be reduced by using low-cost substrates. In this research, attention was focused on the preparation of rhamnolipids, which are biosurfactants, using potential frying edible oils as a carbon source via a microbial fermentation technique. The use of low-cost substrates as a carbon source was emphasized to tilt the cost of production for rhamnolipids. The yield was 2.8 g/L and 7.5 g/L from waste frying oil before and after activated earth treatment, respectively. The crude product contained mainly dirhamnolipids, confirmed by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), liquid chromatography-mass spectroscopy (LC-MS), and (1)H-nuclear magnetic resonance (NMR). Hence, the treatment can be used to convert waste frying oil as a low-cost substrate into a cost-effective carbon source.

Sunflower Acid Oil‐Based Production of Rhamnolipid Using Pseudomonas aeruginosa and Its Application in Liquid Detergents

“…The rhamnolipid structure was confirmed by 1 H NMR analysis and the chemical shifts are presented in Table . The chemicals shifts observed in the NMR spectra were in accordance with the rhamnolipid structure and were similar to those reported in the literature (Pratap et al, ; Sim et al, ; Wadekar et al, ).…”

“…Figure shows the HPLC chromatogram with characteristic peaks at retention times 29.91 and 35.22 min representing the presence of dirhamnolipid and monorhamnolipid, respectively (Jadhav et al, ). It has been reported in the literature that HPLC peaks at 29 and 35 min correspond to anions of dirhamnolipids ( m/z 649) and monorhamnolipids ( m/z 504), respectively (Pratap et al, ; Wadekar et al, ). According to the fragmentation patterns mentioned by Wadekar et al (), the structural assignment of these anions resembles the rhamnolipid structure.…”

“…It has been reported in the literature that HPLC peaks at 29 and 35 min correspond to anions of dirhamnolipids ( m/z 649) and monorhamnolipids ( m/z 504), respectively (Pratap et al, ; Wadekar et al, ). According to the fragmentation patterns mentioned by Wadekar et al (), the structural assignment of these anions resembles the rhamnolipid structure.…”

Rhamnolipids Biosynthesis in Pseudomonas Aeruginosa Onu 301 in the Presence of Exogenous Signaling Quinolone

“…Rhamnolipids separation was performed with TLC method on Alugram Sil G/UV 254 TLC plates (Germany) in chloroform-methanol-water (65:12:2) mixture [14]. Rhamnolipids spots placement was determined by color reaction with rhamnose and acetic acid-sulphuric acid-anis aldehyde solution (50:1:0.05) and TLC plates were heated at 80 °С till pink-orange staining appearance.…”

Kinetics of Dirhamnolipids Biosynthesis and Rhamnosyltransferase 2 Activity in the Presence of Pseudomonas Aeruginosa Signal Quinolone

“…Rhamnolipids separation was performed with TLC method on Alugram Sil G/UV 254 TLC plates (Germany) in chloroform-methanol-water (65:12:2) mixture [14]. Rhamnolipids spots placement was determined by color reaction with rhamnose and acetic acid-sulphuric acid-anis aldehyde solution (50:1:0.05) and TLC plates were heated at 80 о С till appearance of pink-orange staining.…”