Microbial succinic acid production: Natural versus metabolic engineered producers

Beauprez, Joeri; Mey, Marjan De; Soetaert, Wim

doi:10.1016/j.procbio.2010.03.035

Cited by 242 publications

(155 citation statements)

References 118 publications

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“…Various microbial platforms have been explored for bio-production of SA with the best bench-scale performance achieved by Actinobacillus succinogenes [11][12][13][14], Anaerobiospirillum succiniciproducens [15,16], Mannheimia succiniciproducens [17][18][19], and engineered strains of Escherichia coli [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Continuous succinic acid production from xylose by Actinobacillus succinogenes

Bradfield

Nicol

2015

Bioprocess Biosyst Eng

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) and carbohydrate consumption rates were similar. Also, mass balance closures on xylose were up to 18.2% lower than those on glucose. A modified HPLC method revealed pyruvic acid excretion at appreciable concentrations (1.2 -) which improved the mass balance closure by up to 16.8%. Furthermore, redox balances based on the accounted xylose consumed and the excreted metabolites, indicated an overproduction of reducing power. The oxidative pentose phosphate pathway was shown to be a plausible source of the additional reducing power.

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Section: Introductionmentioning

confidence: 99%

Continuous succinic acid production from xylose by Actinobacillus succinogenes

Bradfield

Nicol

2015

Bioprocess Biosyst Eng

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“…The global bio-based SA market is expected to reach 840 million USD by 2018 (TransparencyMarket-Research 2013). Most of the studies performed on SA fermentation employ wild bacterial strains and modified strains of Escherichia coli (Beauprez et al 2010). Due to the CO 2 fixation step in the production of SA it is theoretically possible to obtain 1.12 grams of SA per gram of glucose (van Heerden and Nicol 2013a).…”

Section: Introductionmentioning

confidence: 99%

Succinic acid-producing biofilms of Actinobacillus succinogenes: reproducibility, stability and productivity

Maharaj

Bradfield

Nicol

2014

Appl Microbiol Biotechnol

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Continuous anaerobic fermentations were performed in a biofilm reactor packed with Poraver® beads. Dilution rates (D) varied between 0.054 h -1 and 0.72 h -1 and D-glucose and CO 2 gas were used as carbon substrates. Steady-state conditions were shown to be repeatable and independent of the operational history. Production stability was achieved over periods exceeding 80 h at values of D below 0.32 h -1 . In these situations, steady-state variation (expressed as fluctuations in NaOH neutralisation flow rates) exhibited a standard deviation of less than 5% while no indication of biofilm deactivation was detected. The total biomass amount was found to be independent of the dilution rate with an average dry concentration of

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“…Enzyme activities were studied by analyzing the magnitude of the metabolic fluxes at different glucose ratios. Malic enzyme (ME) and malic dehydrogenase (MDH) are two enzymes responsible for switching the carbon flux between succinate and other products (Beauprez et al, 2010). The metabolic model recognized no oxaloacetate decarboxylase (OADC), ME and alcohol dehydrogenase (AD) activity in the wild type strain when the maximization of growth rate was the objective function.…”

Section: Enzyme Activitymentioning

confidence: 99%

“…It is an important building block with a great economical potential and a precursor for other valuable chemicals. Actinobacillus succinogenes, a facultative anaerobic member of the Pasteurellaceae family, is one of the most promising microorganisms for industrial applications due to its high productivity, and the ability of growth on a broad range of substrates (Beauprez et al, 2010). Recent studies have been conducted using renewable cheap carbon sources for succinic acid production by A .succinogenes to reduce the cost of the end product.…”

Section: Introductionmentioning

confidence: 99%

Metabolic capabilities of Actinobacillus succinogenes for succinic acid production

Rafieenia

2014

Braz. J. Chem. Eng.

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-Attention has been focused on microbial succinic acid production as an alternative for conventional chemical synthesis that is associated with environmental pollution. A metabolic model for Actinobacillus succinogenes 130Z was developed with a mixture of glucose and xylose as substrate. The metabolic fluxes during succinicate production were determined using flux balance analysis by linear programming optimization in the MATLAB environment. Different glucose ratios (0.3, 0.4 and 0.7 mol.mol -1 substrate) were used as model assumptions to calculate optimal fluxes, maximum growth and succinate production. The model revealed that higher growth rates and product yields were correlated with higher glucose content in the substrate mixture. When glucose constituted 0.5 mol.mol -1 substrate, a lower succinate yield (0.64 mol.mol -1 substrate) was obtained, compared to 0.73 mol.mol -1 substrate when glucose was used individually. Deletion of different unessential reactions in the model showed that a knockout of the acetate formation pathway would increase the succinate yield by 21% when glucose and xylose were used in equal molar ratios.

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Microbial succinic acid production: Natural versus metabolic engineered producers

Cited by 242 publications

References 118 publications

Continuous succinic acid production from xylose by Actinobacillus succinogenes

Continuous succinic acid production from xylose by Actinobacillus succinogenes

Succinic acid-producing biofilms of Actinobacillus succinogenes: reproducibility, stability and productivity

Metabolic capabilities of Actinobacillus succinogenes for succinic acid production

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