Microbial Source Tracking Analysis Using Viral Indicators in Santa Lucía and Uruguay Rivers, Uruguay

Bortagaray, Viviana; Lizasoain, Andrés; Piccini, Claudia; Gillman, Luciana; Berois, Mabel; Pou, Sonia Alejandra; Díaz, María del Pilar; Tort, Fernando López; Colina, Rodney; Victoria, Matías

doi:10.1007/s12560-019-09384-2

Cited by 13 publications

(6 citation statements)

References 33 publications

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“…Another surveillance study performed in Negro River, in the Brazilian Amazonic region, HAdV mean viral concentrations were similar to our study (10 6 GC/L) and RVA mean viral concentrations were lower (10 4 -10 5 GC/L) (Girones et al, 2010). Bortagaray et al (2019) found mean viral concentration of 10 4 GC/L of both HAdV and BoPyV in water samples from a river in Uruguay. In a study using water samples from a river in Sweden, HAdV and BoPyV mean viral concentrations were of 5.2 × 10 4 GC/L and 1.0 × 10 2 GC/L, respectively.…”

Section: Discussionsupporting

confidence: 89%

“…In Uruguay, a study also showed HAdV as the most prevalent virus, followed by BoPyV in waters from a basin and a river, with viral concentrations varying from 10 2 to 10 4 GC/L. The authors also showed that HAdV particles from analyzed samples were infective (Bortagaray et al, 2019). In our study, RVA was detected less frequently than HAdV but presented higher viral concentrations (up to 10 9 GC/L).…”

Section: Discussionsupporting

confidence: 66%

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Investigation of Human and Animal Viruses in Water Matrices from a Rural Area in Southeastern Region of Brazil and Their Potential Use as Microbial Source-Tracking Markers

et al. 2023

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This study assessed the sources of contamination of water matrices in a rural area using detection of a host-specific virus (human adenovirus [HAdV], porcine adenovirus [PAdV] and bovine polyomaviruses [BoPyV]) as potential microbial sourcetracking tool, and rotavirus A [RVA], given its epidemiological importance in Brazil. From July 2017 to June 2018, 92 samples were collected from eight points (P1-P8) of surface and raw waters in southeastern region of Brazil. Fifty-five (59.8%) were positive for HAdV, 41 (44.5%) for RVA, 10 (10.9%) for PAdV and four (4.3%) for BoPyV. HAdV and RVA were detected at all sites, and over the entire sampling period, PAdV was detected at a porcine breeding area and at Guarda River site, presenting high concentrations up to 2.6 × 10 9 genome copies per liter [GC/L], and viral concentrations ranging from 9.6 × 10 1 to 7.1 × 10 7 , while BoPyV (1.5 × 10 4 GC/L-9.2 × 10 5 GC/L) was only detected in samples from the bovine breeding areas. The combination of human and animal virus circulation presents a potential impact in the environment due to raw sewage discharge from regional communities, as well as potential hazard to human and animal health.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 66%

Investigation of Human and Animal Viruses in Water Matrices from a Rural Area in Southeastern Region of Brazil and Their Potential Use as Microbial Source-Tracking Markers

et al. 2023

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“…Here, the review of global studies suggests that AdVs, AiV, FRNAP-II, FRNAP-III, crAssphage and PMMoV are detected more frequently and at high concentrations in wastewater and within polluted water bodies than the other indicators reviewed. PyVs are also present in wastewater at high concentrations, however, they are less prevalent in the environment than AdV (Albinana-Gimenez et al, 2009;Bortagaray et al, 2019;Dias et al, 2018a, b;Haramoto et al, 2010;Moresco et al, 2012), suggesting rapid degradation. Similarly, while FRNAP-II and FRNAP-III are initially found at high concentrations in wastewater, it is likely that they degrade more rapidly in the environment than AdV.…”

Section: Conclusion and Future Researchmentioning

confidence: 99%

Viral indicators for tracking domestic wastewater contamination in the aquatic environment

et al. 2020

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Section: Introductionmentioning

confidence: 99%

“…Molecular tools haves been increasingly used in environmental microbiology, bacterial ecology and in environmental monitoring of pathogens and toxins (Martínez de la Escalera et al, 2017; Bortagaray et al, 2019;Kumar et al, 2021). However, in the assessment of fecal contamination of beaches, these tools have only been used for the assessment of water samples in Uruguay (Bortagaray et al, 2019(Bortagaray et al, , 2020, where monitoring efforts of fecal contamination in sand samples have been scarce. Although some research works have incorporated the analysis of soil samples, including sediment, sand or intertidal beach bio lms for monitoring the environment using molecular techniques (Martínez de la Escalera, 2014; Piccini & García-Alonso, 2015), few studies have been focused on molecular monitoring of fecal contamination (Soumastre et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Molecular-based approach for monitoring fecal contamination in sand from recreational beaches

Cabot,

Piccini,

Inchausti

et al. 2023

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For decades, the risks of catching infectious diseases in recreational beaches has been addressed by measuring abundance of fecal indicator bacteria from water samples through culture methods. Recently, analyses of sand samples for beach quality assessments have been developed as a complement for monitoring recreational waters. In parallel, the growing use of molecular techniques for environmental monitoring has led to obtaining rapid results on the exposure to fecal contamination and the detection of pathogenic genes directly. The aim of this work was to analyze the fecal contamination of sand from touristic beaches by qPCR, together with abundance of thermotolerant coliforms in sand and water. The results revealed the presence of pathogenic genes. Pathogenic genes (mainly stx1) were confirmed in 8 out of 20 samples, associated with the lack of sanitation network. The recreational use of sandy beaches may imply a risk to the health of its users, especially near streams and creek outflows, demonstrating the importance of monitoring sand bacteriological quality, and the usefulness of directly monitoring pathogens by means of molecular tools.

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Microbial Source Tracking Analysis Using Viral Indicators in Santa Lucía and Uruguay Rivers, Uruguay

Cited by 13 publications

References 33 publications

Investigation of Human and Animal Viruses in Water Matrices from a Rural Area in Southeastern Region of Brazil and Their Potential Use as Microbial Source-Tracking Markers

Investigation of Human and Animal Viruses in Water Matrices from a Rural Area in Southeastern Region of Brazil and Their Potential Use as Microbial Source-Tracking Markers

Viral indicators for tracking domestic wastewater contamination in the aquatic environment

Molecular-based approach for monitoring fecal contamination in sand from recreational beaches

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