Microbial removal of lead from solid media and soil

Vesper, Stephen; Donovan-Brand, Rebecca; Paris, K. Pete; Al-Abed, Souhail R.; Ryan, James A.; Davis-Hoover, W.J.

doi:10.1007/bf00279157

Cited by 14 publications

(8 citation statements)

References 7 publications

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“…120S. these results are in agreement with that described by Stephen et al (1996) who found that cells of P. aeruginosa CHL004 pretreated with sodium azide did not remove lead,…”

Section: Effect Of Metabolic Inhibitorssupporting

confidence: 95%

Biosorption of nickel by Pseudomonas cepacia 120S and Bacillus subtilis 117S

Abd‐Elmonem

AL-Zubeiry

Al-Gheethi

2010

Water Science and Technology

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Biosorption of nickel by two bacterial species: Bacillus subtilis 117S and Pseudomonas cepacia 120S was studied. The maximum uptake of nickel was achieved at 234.4 microg Ni2+ ml(-1) by P. cepacia 120S (living and dead biomass) and at 117.2 and 351.6 microg Ni2+ ml(-1) by living and dead biomass of B. subtilis 117S. The increase in biomass concentration has shown an increase in the nickel uptake. The nickel removal increased significantly during contact time from 1 to 8 h then remained constant until 24 h where the equilibrium occurred. Biosorption efficiency of nickel increased with increasing pH from 2 to 7 for living and dead biomass of P. cepacia 120S and B. subtilis 117S. Temperature had an important role in nickel biosorption by both species. The nickel removal by living biomass was significantly disturbed after pretreatment of bacterial biomass with sodium azide, mercuric chloride and formaldehyde. Esterification of carboxyl groups, methylation of amino groups and extraction of lipid fraction of biomass by acetone and benzene significantly reduced the biosorption capacity of nickel. Repeated biosorption and desorption operations exhibited that the biosorption capacity of bacterial biomass regenerated with HNO3 and NaOH as desorbing medium increased significantly in cycle 4 for P. cepacia 120S and B. subtilis 117S. In case of regeneration with HNO3 and distilled water the biosorption capacity increased significantly in cycle 4 for B. subtilis 117S and did not differ significantly from cycle 1 to cycle 4 for P. cepacia 120S. The biosorption capacity of living and dead biomass of B. subtilis 117S and dead biomass of P cepacia 120S (155.5 as compared to 175.6 and 169.8 mg Ni2+ g(-1)) was higher than that of sludge, tea and saw dust (148.4, 52.7 and 44.6 mg Ni2+ g(-1)).

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“…120S. these results are in agreement with that described by Stephen et al (1996) who found that cells of P. aeruginosa CHL004 pretreated with sodium azide did not remove lead,…”

Section: Effect Of Metabolic Inhibitorssupporting

confidence: 95%

Biosorption of nickel by Pseudomonas cepacia 120S and Bacillus subtilis 117S

Abd‐Elmonem

AL-Zubeiry

Al-Gheethi

2010

Water Science and Technology

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“…Microorganisms and microbial products have been reported to efficiently remove soluble and particulate forms of metals, especially from dilute solutions, though bioaccumulation and therefore microbe-based technologies provide an alternative to the conventional techniques of metal removal/recovery (Ozdemir et al 2004). Microorganisms change/reduce the toxicity of metallic contaminants through pH changes, bioaccumulation or biosorption (Al-shahwani et al 1984;Vesper et al 1996). Microbes are capable of accumulating toxic metal ions by two well defined processes viz: (i) biosorption: an energy-independent binding of metal ions to cell walls and (ii) bioaccumulation: energy-dependent process of metal uptake into the cells.…”

Section: Introductionmentioning

confidence: 98%

Isolation and Characterization of A Metal-resistant Pseudomonas Aeruginosa Strain

Raja

Anbazhagan

Selvam

2006

World J Microbiol Biotechnol

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The use of microorganisms to remove heavy metals from industrial effluent is an area of extensive research and development. Attempts have been made to isolate and characterize metal-resistant microorganisms from treated oil mill industry effluent wastewater samples. The metal-resistant organisms that showed values of minimum inhibitory concentration towards metals (Cd, Cr, Ni and Pb) ranging from 100 to 800 ppm level were screened. A potent metal-resistant organism, isolate BC15 from the wastewater samples was tentatively identified as Pseudomonas sp. Detailed analysis of morphological, biochemical and 16S rDNA sequence of the isolate revealed that it is closely related to Pseudomonas aeruginosa (94%). Pseudomonas BC15 was capable of absorbing 93% Ni, 65% Pb, 50% Cd and 30% Cr within 48 h from the medium containing 100 mg of each heavy metal per liter. The multiple metal tolerance of this strain was also associated with resistance to antibiotics such as ampicillin, tetracycline, chloramphenicol, erythromycin, kanamycin and streptomycin.

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“…Bacteria are often involved in the fate and transport of toxic metals in soil (35,36,50,62). Both abiotic and biotic processes catalyze the reduction of Cr(VI) to Cr(III) in the natural environment (20), but bacterial reduction of Cr(VI) to Cr(III) can occur under nutrient-amended vadose zone conditions, which implies that biostabilization is a viable management option (42).…”

mentioning

confidence: 99%

Enhanced Exopolymer Production and Chromium Stabilization inPseudomonas putidaUnsaturated Biofilms

Priester

Olson

Webb

et al. 2006

Appl Environ Microbiol

201

102

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Chromium-contaminated soils threaten surface and groundwater quality at many industrial sites. In vadose zones, indigenous bacteria can reduce Cr(VI) to Cr(III), but the subsequent fate of Cr(III) and the roles of bacterial biofilms are relatively unknown. To investigate, we cultured Pseudomonas putida, a model organism for vadose zone bioremediation, as unsaturated biofilms on membranes overlaying iron-deficient solid media either containing molecular dichromate from potassium dichromate (Cr-only treatment) or with deposits of solid, dichromate-coated hematite (Fe؉Cr treatment) to simulate vadose zone conditions. Controls included iron-deficient solid medium and an Fe-only treatment using solid hematite deposits. Under iron-deficient conditions, chromium exposure resulted in lower cell yield and lower amounts of cellular protein and carbohydrate, but providing iron in the form of hematite overcame these toxic effects of Cr. For the Cr and Fe؉Cr treatments, Cr(VI) was completely reduced to Cr(III) that accumulated on biofilm cells and extracellular polymeric substances (EPSs). Chromium exposure resulted in elevated extracellular carbohydrates, protein, DNA, and EPS sugars that were relatively enriched in N-acetyl-glucosamine, rhamnose, glucose, and mannose. The proportions of EPS protein and carbohydrate relative to intracellular pools suggested Cr toxicity-mediated cell lysis as the origin. However, DNA accumulated extracellularly in amounts far greater than expected from cell lysis, and Cr was liberated when extracted EPS was treated with DNase. These results demonstrate that Cr accumulation in unsaturated biofilms occurs with enzymatic reduction of Cr(VI), cellular lysis, cellular association, and extracellular DNA binding of Cr(III), which altogether can facilitate localized biotic stabilization of Cr in contaminated vadose zones.

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Microbial removal of lead from solid media and soil

Cited by 14 publications

References 7 publications

Biosorption of nickel by Pseudomonas cepacia 120S and Bacillus subtilis 117S

Biosorption of nickel by Pseudomonas cepacia 120S and Bacillus subtilis 117S

Isolation and Characterization of A Metal-resistant Pseudomonas Aeruginosa Strain

Enhanced Exopolymer Production and Chromium Stabilization inPseudomonas putidaUnsaturated Biofilms

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