Microbial Reduction of Chromate in the Presence of Nitrate by Three Nitrate Respiring Organisms

Chovanec, Peter; Sparacino-Watkins, Courtney; Zhang, Ning; Basu, Partha; Stolz, John F.

doi:10.3389/fmicb.2012.00416

Cited by 42 publications

(18 citation statements)

References 62 publications

(64 reference statements)

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“…Like nitrate, perchlorate can serve as an electron acceptor in biology. 19 For example, an elevated nitrate level has been associated with chromium, 20,21 and uranium. 22,23 Thus successful strategies for the bioremediation of heavy metal contaminated sites must address the influence of nitrate in the process.…”

Section: Nitrate Reductionmentioning

confidence: 99%

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Nitrate and periplasmic nitrate reductases

2014

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The nitrate anion is a simple, abundant and relatively stable species, yet plays a significant role in global cycling of nitrogen, global climate change, and human health. Although it has been known for quite some time that nitrate is an important species environmentally, recent studies have identified potential medical applications. In this respect the nitrate anion remains an enigmatic species that promises to offer exciting science in years to come. Many bacteria readily reduce nitrate to nitrite via nitrate reductases. Classified into three distinct types – periplasmic nitrate reductase (Nap), respiratory nitrate reductase (Nar) and assimilatory nitrate reductase (Nas), they are defined by their cellular location, operon organization and active site structure. Of these, Nap proteins are the focus of this review. Despite similarities in the catalytic and spectroscopic properties Nap from different Proteobacteria are phylogenetically distinct. This review has two major sections: in the first section, nitrate in the nitrogen cycle and human health, taxonomy of nitrate reductases, assimilatory and dissimilatory nitrate reduction, cellular locations of nitrate reductases, structural and redox chemistry are discussed. The second section focuses on the features of periplasmic nitrate reductase where the catalytic subunit of the Nap and its kinetic properties, auxiliary Nap proteins, operon structure and phylogenetic relationships are discussed.

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Section: Nitrate Reductionmentioning

confidence: 99%

“…22,23 Thus successful strategies for the bioremediation of heavy metal contaminated sites must address the influence of nitrate in the process. 21 …”

Section: Nitrate Reductionmentioning

confidence: 99%

Nitrate and periplasmic nitrate reductases

2014

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“…Interestingly, Geobacter species have been reported previously to reduce toxic hexavalent chromium [Cr(VI)] to trivalent chromium [Cr(III)] (Chovanec et al 2012;Tuo et al 2013), thus this community member may have an ability to resist this toxin. Within the dominant phyla Bacteroidetes and Proteobacteria, several OTUs correlated positively with Zn, which has been documented previously in another study (Azarbad et al 2015).…”

Section: Impact Of Available Metal On Microbial Otusmentioning

confidence: 99%

High concentrations of bioavailable heavy metals impact freshwater sediment microbial communities

Horton

Rui

et al. 2015

Ann Microbiol

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Heavy metal concentrations within freshwater systems can increase as a result of anthropogenic inputs, which in turn can influence microbial community composition and community function. In this study, lake sediments collected from two historically heavy metal impacted geographical regions [Poyang Lake, People's Republic of China (PRC), and sites adjacent to the Muskegon Watershed, MI (USA)] were analyzed for total and available heavy metals (Cd, Cr, Cu, Pb, and Zn), as well as microbial community structure using highthroughput sequencing technology. In PRC tributaries leading into Poyang Lake, Zn was found to be the most available metal species. In USA sites, available Cd was higher in Mona Lake sites than alternate USA sites. In both regions, results indicate a weak influence of metals on microbial alpha and beta diversity, but strong positive and negative correlations of available heavy metals with specific taxonomic groups of bacteria and archaea. Further, individual metal species impacted microbial groups and operational taxonomic units differently, suggesting that individual metal species are important in determining which microbial groups and species prevail under heavy metal stress, particularly in the case of bioavailable metals. In PRC, several taxonomic groups were correlated positively with Zn, including Actinobacteria, Bacteroidetes, Proteobacteria, and Verrucomicrobia. Geobacter and Pedosphaerales were both correlated positively to Cr in the same sites, while other correlations with microbial groups were negative. In sites from the USA, Deltaproteobacteria were correlated positively with Cd, while Betaproteobacteria were negatively correlated, suggesting that Cd resistance may differ at the level of Class within Proteobacteria. This study highlights the importance of analyzing available metals when examining the impact of this disturbance on microbial community structure within aquatic sediments.

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“…The host bacteria with a broad adaptability to the environment can be selected for future studies. In biodegradation, the main problems to be solved are the effects of other pollutants on reducing Cr (VI), in which the most common is the interference of metal ions (20). In the present study, it was shown that the majority of common metal ions did not inhibit the Cr (VI) reduction in engineered bacteria, whereas Cu 2+ , Co 2+ and Pb…”

Section: Discussionmentioning

confidence: 71%

“…The Cr (VI) concentration in the medium at different culture temperatures (20,25, 30, 37, 40 and 50˚C) was also detected. As shown in Fig.…”

Section: Effects On Cr (Vi) Elimination Capability By the Culturing Cmentioning

confidence: 99%

Reducing capacity and enzyme activity of chromate reductase in a ChrT-engineered strain

Zhou

Dong

Deng³

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. In order to remediate the metal-contaminated soil and water ecosystems with microorganisms, an engineered strain, which contained the chromate reductase ChrT gene from Serratia sp. S2, was studied in detail for its Cr (VI) reduction efficiency, optimal culture condition and chromate reductase activity. Results demonstrated that the engineered strain had a high Cr (VI) reduction rate of up to 40% at a concentration of 50 mg/l after being cultured for 48 h. Additionally, the optimal culture conditions were pH 7.0 and 37˚C. Furthermore, the carbon sources and metal cations exhibited significant effects on the Cr (VI) reduction rate of the engineered bacterium. Sodium lactate, sodium acetate, Cu 2+ , Co 2+ and Pb 2+ were positively correlated with the reduction rate. Chromate reductase was soluble and presented in the cytoplasm. Furthermore, the enzymatic activity with nicotinamide adenine dinucleotide phosphate, which was as an electron donor, reached 14.83 U/mg.

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Microbial Reduction of Chromate in the Presence of Nitrate by Three Nitrate Respiring Organisms

Cited by 42 publications

References 62 publications

Nitrate and periplasmic nitrate reductases

Nitrate and periplasmic nitrate reductases

High concentrations of bioavailable heavy metals impact freshwater sediment microbial communities

Reducing capacity and enzyme activity of chromate reductase in a ChrT-engineered strain

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