Microbial redox coenzyme engineering and applications in biosynthesis

Evaluation and comparison of antioxidant abilities of five bioactive molecules with C–H and O–H bonds in thermodynamics and kinetics

“…BNAH, F420H, and iAscH − are not only good hydrogen atom donors, but also important hydride transporters in vivo . NAD coenzyme is hydrogenase as its main function, 19 while F420 coenzyme can perform both dehydrogenase and hydrogenase functions in vivo . 20,21 In Scheme 3, the heterolytic bond dissociation free energies Δ G ° (H − X) of BNAH, F420H, and iAscH − in acetonitrile are also listed, which are 59.30 kcal mol −1 (BNAH), 71.10 kcal mol −1 (F420H), and 75.70 kcal mol −1 (iAscH − ), respectively.…”

Surface-modified bacteria: synthesis, functionalization and biomedical applications

“…108 Initially, the adsorption of metal ions by electrostatic attraction occurs on the cell wall through the carboxyl and phosphate groups. Subsequently, the bioactive substances that are secreted by microorganisms, such as coenzyme I nicotinamide adenine dinucleotide (NADH), 109 reduced coenzyme II (NADPH), 109 nitrate reductase, 110,111 phytochelatins, 110 hydrogenases, 112 and glutathione, 113 situated on the cell wall or membrane, act as electron donors. This process results in the reduction of high-valent metal ions into less watersoluble or less toxic singlet or low-valent metals.…”

Optimal energy and redox metabolism in the cyanobacterium Synechocystis sp. PCC 6803

“…In addition, re-oxidation of NAD(P)H to NAD(P) + and phosphorylation-dephosphorylation of reducing equivalents are important for balancing the ATP/NADPH budget and maintaining redox homeostasis 15 . Despite the importance of meeting the energy and redox requirement to achieve an efficient cellular performance 16 , 17 , only few metabolic engineering efforts of Synechocystis have targeted this designing strategy 18 , while most studies are concerned with controlling carbon partitioning 1 .…”