Microbial production of <scp>D</scp>‐mannitol and <scp>D</scp>‐fructose from glycerol

Ōnishi, Hiroshi; Suzuki, Toshiyuki

doi:10.1002/bit.260120605

Cited by 25 publications

(4 citation statements)

References 13 publications

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“…In shake flask culture C. magnoliae secreted 67 g dm −3 of mannitol from fructose (~ 0.45 g g −1 ; [ 56 ]). Although microbiological production of mannitol is not as popular as erythritol, it is known that apart from C. magnoliae also Candida zeylanoides , Torulopsis mannitofaciens and Torulopsis versalitis are able to produce this compound [ 13 , 40 , 56 ]. Even less popular is the microbiological production of arabitol.…”

Section: Resultsmentioning

confidence: 99%

High value-added products derived from crude glycerol via microbial fermentation using Yarrowia clade yeast

et al. 2021

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Background Contemporary biotechnology focuses on many problems related to the functioning of developed societies. Many of these problems are related to health, especially with the rapidly rising numbers of people suffering from civilization diseases, such as obesity or diabetes. One factor contributing to the development of these diseases is the high consumption of sucrose. A very promising substitute for this sugar has emerged: the polyhydroxy alcohols, characterized by low caloric value and sufficient sweetness to replace table sugar in food production. Results In the current study, yeast belonging to the Yarrowia clade were tested for erythritol, mannitol and arabitol production using crude glycerol from the biodiesel and soap industries as carbon sources. Out of the 13 tested species, Yarrowia divulgata and Candida oslonensis turned out to be particularly efficient polyol producers. Both species produced large amounts of these compounds from both soap-derived glycerol (59.8–62.7 g dm−3) and biodiesel-derived glycerol (76.8–79.5 g dm−3). However, it is equally important that the protein and lipid content of the biomass (around 30% protein and 12% lipid) obtained after the processes is high enough to use this yeast in the production of animal feed. Conclusions The use of waste glycerol for the production of polyols as well as utilization of the biomass obtained after the process for the production of feed are part of the development of modern waste-free technologies.

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Section: Resultsmentioning

confidence: 99%

High value-added products derived from crude glycerol via microbial fermentation using Yarrowia clade yeast

et al. 2021

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“…Microbial production of mannitol is reported with bacteria, yeasts, and filamentous fungi. With yeast and filamentous fungi moderate to good mannitol yields from sugar raw materials (glucose, glycerol, or sucrose) have been reported (8)(9)(10)(11). The production rates (productivities) are, however, low.…”

Section: Introductionmentioning

confidence: 99%

Scale-up of a New Bacterial Mannitol Production Process

Weymarn¹,

Kiviharju²,

Jääskeläinen³

et al. 2003

Biotechnol. Prog.

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D-Mannitol is a sugar alcohol with applications in chemistry, food and pharmaceutical industries, and medicine. Commercially, mannitol is produced by catalytic hydrogenation. Although this process is widely used, it is not optimal for mannitol production. New processes, including chemical, enzymatic, and microbial processes, are frequently developed and evaluated against the existing hydrogenation processes. In earlier papers, we have described the identification of a food-grade lactic acid bacterium strain, Leuconostoc mesenteroides ATCC-9135, with efficient mannitol production capabilities and the development and optimization of a new bioprocess in which the strain was applied. The new bioprocess is simple. It requires a reduced bioreactor with the following features: sterilization, pH and T control (at mild conditions), and slow mixing. The contamination risk of the new bioprocess is low, and the downstream processing protocol comprises simple, widely used unit operations: evaporation, crystallization, crystal separation, and drying. On a 2-L laboratory scale, high mannitol yields from fructose (93-97%) and volumetric mannitol productivities (>20 g L(-1) h(-1)) were achieved. In this paper, the scalability of the new bioprocess was tested on a small pilot scale (100 L). In the pilot plant, production levels were achieved similar to those in the laboratory. Also, high-purity mannitol crystals were obtained at similar yield levels. The results presented in this paper indicate that the new bioprocess can easily be scaled-up to an industrial scale and that the production levels achieved with it are comparable to the catalytic hydrogenation processes.

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“…Some factors that influence the ability of two yeasts ( Torulopsis mannitofaciens CBS 5981 and T. versatilis CBS 1752) to produce mannitol from glycerol have been investigated [100]. In optimal conditions, T. mannitofaciens produced mannitol from glycerol consumed with a yield of 31% of the theorethycal maximum.…”

Section: Introductionmentioning

confidence: 99%

Biodiesel biorefinery: opportunities and challenges for microbial production of fuels and chemicals from glycerol waste

Almeida

Fávaro

Quirino

2012

Biotechnol Biofuels

201

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The considerable increase in biodiesel production worldwide in the last 5 years resulted in a stoichiometric increased coproduction of crude glycerol. As an excess of crude glycerol has been produced, its value on market was reduced and it is becoming a “waste-stream” instead of a valuable “coproduct”. The development of biorefineries, i.e. production of chemicals and power integrated with conversion processes of biomass into biofuels, has been singled out as a way to achieve economically viable production chains, valorize residues and coproducts, and reduce industrial waste disposal. In this sense, several alternatives aimed at the use of crude glycerol to produce fuels and chemicals by microbial fermentation have been evaluated. This review summarizes different strategies employed to produce biofuels and chemicals (1,3-propanediol, 2,3-butanediol, ethanol, n-butanol, organic acids, polyols and others) by microbial fermentation of glycerol. Initially, the industrial use of each chemical is briefly presented; then we systematically summarize and discuss the different strategies to produce each chemical, including selection and genetic engineering of producers, and optimization of process conditions to improve yield and productivity. Finally, the impact of the developments obtained until now are placed in perspective and opportunities and challenges for using crude glycerol to the development of biodiesel-based biorefineries are considered. In conclusion, the microbial fermentation of glycerol represents a remarkable alternative to add value to the biodiesel production chain helping the development of biorefineries, which will allow this biofuel to be more competitive.

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Microbial production of D‐mannitol and D‐fructose from glycerol

Cited by 25 publications

References 13 publications

High value-added products derived from crude glycerol via microbial fermentation using Yarrowia clade yeast

High value-added products derived from crude glycerol via microbial fermentation using Yarrowia clade yeast

Scale-up of a New Bacterial Mannitol Production Process

Biodiesel biorefinery: opportunities and challenges for microbial production of fuels and chemicals from glycerol waste

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