Microbial production of riboflavin using riboflavin overproducers,Ashbya gossypii, Bacillus subtilis, andCandida famate: An overview

Lim, Seong Han; Choi, Jong Soo; Park, Enoch Y.

doi:10.1007/bf02931951

Cited by 60 publications

(56 citation statements)

References 102 publications

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“…With A. gossypii, much attention was paid to optimization of fermentation conditions favoring RF oversynthesis (83,268). RF overproduction occurs on different residues, including oily waste-activated bleaching earth (490).…”

Section: A Gossypiimentioning

confidence: 99%

“…Selection for resistance to RF analogs was not reported, apparently due to the resistance of A. gossypii to these compounds (462). No information is available on A. gossypii mutants resistant to analogs of purines, although exogenous purines and the purine precursor glycine activate RF production (268). As oil stimulates RF production in A. ashbyii, activation of the glyoxylic acid cycle should be important for strain improvement.…”

Section: A Gossypiimentioning

confidence: 99%

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Genetic Control of Biosynthesis and Transport of Riboflavin and Flavin Nucleotides and Construction of Robust Biotechnological Producers

Abbas

Sibirny

2011

Microbiol Mol Biol Rev

316

272

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SUMMARY Riboflavin [7,8-dimethyl-10-(1′- d -ribityl)isoalloxazine, vitamin B 2 ] is an obligatory component of human and animal diets, as it serves as the precursor of flavin coenzymes, flavin mononucleotide, and flavin adenine dinucleotide, which are involved in oxidative metabolism and other processes. Commercially produced riboflavin is used in agriculture, medicine, and the food industry. Riboflavin synthesis starts from GTP and ribulose-5-phosphate and proceeds through pyrimidine and pteridine intermediates. Flavin nucleotides are synthesized in two consecutive reactions from riboflavin. Some microorganisms and all animal cells are capable of riboflavin uptake, whereas many microorganisms have distinct systems for riboflavin excretion to the medium. Regulation of riboflavin synthesis in bacteria occurs by repression at the transcriptional level by flavin mononucleotide, which binds to nascent noncoding mRNA and blocks further transcription (named the riboswitch). In flavinogenic molds, riboflavin overproduction starts at the stationary phase and is accompanied by derepression of enzymes involved in riboflavin synthesis, sporulation, and mycelial lysis. In flavinogenic yeasts, transcriptional repression of riboflavin synthesis is exerted by iron ions and not by flavins. The putative transcription factor encoded by SEF1 is somehow involved in this regulation. Most commercial riboflavin is currently produced or was produced earlier by microbial synthesis using special selected strains of Bacillus subtilis , Ashbya gossypii , and Candida famata . Whereas earlier RF overproducers were isolated by classical selection, current producers of riboflavin and flavin nucleotides have been developed using modern approaches of metabolic engineering that involve overexpression of structural and regulatory genes of the RF biosynthetic pathway as well as genes involved in the overproduction of the purine precursor of riboflavin, GTP.

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Section: A Gossypiimentioning

confidence: 99%

Section: A Gossypiimentioning

confidence: 99%

Genetic Control of Biosynthesis and Transport of Riboflavin and Flavin Nucleotides and Construction of Robust Biotechnological Producers

Abbas

Sibirny

2011

Microbiol Mol Biol Rev

316

272

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“…Eremothecium gossypii) has recently emerged as an attractive cell factory to produce yet unexplored high-value products. Industrially exploited for more than 20 years due to its natural ability to overproduce riboflavin, A. gossypii is considered a remarkable example of the sustainable white biotechnology business model (Kato and Park, 2012;Lim et al, 2001;Stahmann et al, 2000;zu Berstenhorst et al, 2009). Nevertheless, the scientific prominence of A. gossypii is not limited to its biotechnological potential, since it is also extensively used as a model organism in fungal developmental and evolutionary biology studies (Perez-Nadales et al, 2014;Schmitz and Philippsen, 2011;Wendland and Walther, 2005).…”

Section: Introductionmentioning

confidence: 98%

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Aguiar

Silva

Domingues

2015

Biotechnology Advances

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The filamentous fungus Ashbya gossypii has been safely and successfully used for more than two decades in the commercial production of riboflavin (vitamin B2). Its industrial relevance combined with its high genetic similarity with Saccharomyces cerevisiae together promoted the accumulation of fundamental knowledge that has been efficiently converted into a significant molecular and in silico toolbox for its genetic engineering. This synergy has enabled a directed and sustained exploitation of A. gossypii as an industrial riboflavin producer. Although there is still room for optimizing riboflavin production, the recent years have seen an abundant advance in the exploration of A. gossypii for other biotechnological applications, such as the production of recombinant proteins, single cell oil and flavour compounds. Here, we will address the biotechnological potential of A. gossypii beyond riboflavin production by presenting (a) a physiological and metabolic perspective over this fungus; (b) the molecular toolbox available for its manipulation; and (c) commercial and emerging biotechnological applications for this industrially important fungus, together with the approaches adopted for its engineering.

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“…Currently, vitamin B 2 is produced on a large scale by both chemical and biotechnological synthesis, with the latter gaining more additional significance as a result of the benefits of biotechnological processes like cost effectiveness, less energy is needed, reduction in wastes, and therefore the use of renewable feed stocks (Vandamme 1992;Stahmann et al 2000;Lim et al 2001). Recently, many researchers turn their attention to biological metabolism to improve the production of bioproducts and the efficient fermentation process have replaced chemical synthesis for manufacturing the vitamin (Lehmann et al 2009).…”

Section: Introductionmentioning

confidence: 97%

“…Candida flareri, was able to produce about 600 mg/l of riboflavin but it was necessary to overcome iron inhibition. Unlike in yeast, iron inhibition is not a factor for the two molds, E. ashbyii and A. gossypii, which are natural overproducers of riboflavin, and currently produce riboflavin levels exceeding 20 g/l (Lim et al 2001). Candida famata, as the unicellular yeast, is easier to distribute in a large-scale fermentor than a filamentous fungus growing in mycelia pellets.…”

Section: Introductionmentioning

confidence: 98%

Improvement of medium components for high riboflavin production by Aspergillus terreus using response surface methodology

Nafady

Bagy

Abd‐Alla

et al. 2015

Rend. Fis. Acc. Lincei

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The current study was designed to improve riboflavin production utilizing the filamentous fungus Aspergillus terreus. The effect of different nutritional and environmental factors was carried out by applying onefactor-at-a-time method. The selected high producer fungal strain was identified on the bases of the sequences of the gene encoding 18S rRNA and designated as A. terreus ASU1 (KP793450). In order to investigate the quantitative, optimal levels and the reciprocal interactions between five main medium components, response surface methodology (RSM) was subsequently employed. The results proved that optimal concentrations for enhancement of riboflavin production were: corn starch 30 (g/l); tryptone 0.5 (g/l) and 1 (g/l) for each glycine, KH 2 PO 4 and MgSO 4 . The maximum riboflavin production of 344.60 mg/l (predicted 342.86 mg/l) was determined under the optimal conditions and the regression analysis (R 2 ) of RSM showed 97.7 %. In comparison to the production of original level (63 mg/l), 5.4-fold increase had been obtained.

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Microbial production of riboflavin using riboflavin overproducers,Ashbya gossypii, Bacillus subtilis, andCandida famate: An overview

Cited by 60 publications

References 102 publications

Genetic Control of Biosynthesis and Transport of Riboflavin and Flavin Nucleotides and Construction of Robust Biotechnological Producers

Genetic Control of Biosynthesis and Transport of Riboflavin and Flavin Nucleotides and Construction of Robust Biotechnological Producers

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Improvement of medium components for high riboflavin production by Aspergillus terreus using response surface methodology

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