Microbial production of poly-β-hydroxybutyrate by marine microbes isolated from various marine environments

Arun, A.; Arthi, R; Shanmugabalaji, Venkatasalam; Eyini, M.

doi:10.1016/j.biortech.2008.08.037

Cited by 63 publications

(35 citation statements)

References 17 publications

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“…Stretch of bands ranging from 1057-1277 showed C-O bonding. The analyzed results correlated with the report of Arun et al 2009 confirming that the isolated compound was PHB. …”

Section: Fourier Transforms Infrared Spectroscopy (Ftir) Spectra Analsupporting

confidence: 78%

“…These findings were co-related with the findings of Arun et al 2009 for 1 H NMR as spectra was not shown and values for 13C were missing and the assumed structure from the spectra of 13C NMR relates with structure suggested by Redzwan et al 1997 and confirming that the isolated compound is PHB. …”

Section: Uv-spectrophotometer Analysis (Perkinelmer Lambda 35 Uv/vis supporting

confidence: 61%

“…Mangrove environments or the sediments near to mangrove contain accumulated PHB within Vibrios as the studies conducted by Chien et al 2007. Arun et al 2009 found that along with Vibrios, Bacillus cereus and mycoides also have an ability to accumulate PHB from mangrove environments of south west Asia (Kerala). Very few studies from Saudi Arabia have shown to produce PHB from sources like soil and water using date palm syrup (Khiyami et al 2011).…”

Section: Resultsmentioning

confidence: 99%

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Extraction and Characterization of Polyhydroxybutyrates (PHB) from Bacillus thuringiensisKSADL127 Isolated from Mangrove Environments of Saudi Arabia

Alarfaj

Arshad

Sholkamy

et al. 2015

Braz. arch. biol. technol.

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“…Stretch of bands ranging from 1057-1277 showed C-O bonding. The analyzed results correlated with the report of Arun et al 2009 confirming that the isolated compound was PHB. …”

Section: Fourier Transforms Infrared Spectroscopy (Ftir) Spectra Analsupporting

confidence: 78%

Section: Uv-spectrophotometer Analysis (Perkinelmer Lambda 35 Uv/vis supporting

confidence: 61%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Extraction and Characterization of Polyhydroxybutyrates (PHB) from Bacillus thuringiensisKSADL127 Isolated from Mangrove Environments of Saudi Arabia

Alarfaj

Arshad

Sholkamy

et al. 2015

Braz. arch. biol. technol.

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show abstract

“…Since they have the advantage of being cultivated easily for massive production, bacteria are the main targeted organisms for this purpose (10). In recent years, many marine bacteria have been reported to be able to produce PHAs (2,13,29,30,35). Some bacteria have been employed as sewerage scavengers (4).…”

mentioning

confidence: 99%

Formation of Polyhydroxyalkanoate in Aerobic Anoxygenic Phototrophic Bacteria and Its Relationship to Carbon Source and Light Availability

Xiao

Jiao

2011

Appl Environ Microbiol

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Aerobic anoxygenic phototrophic bacteria (AAPB) are unique players in carbon cycling in the ocean. Cellular carbon storage is an important mechanism regulating the nutrition status of AAPB but is not yet well understood. In this paper, six AAPB species (Dinoroseobacter sp. JL1447, Roseobacter denitrificans OCh 114, Roseobacter litoralis OCh 149, Dinoroseobacter shibae DFL 12 T , Labrenzia alexandrii DFL 11 T , and Erythrobacter longus DSMZ 6997) were examined, and all of them demonstrated the ability to form the carbon polymer polyhydroxyalkanoate (PHA) in the cell. The PHA in Dinoroseobacter sp. JL1447 was identified as poly-betahydroxybutyrate (PHB) according to evidence from Fourier transform infrared spectroscopy, differential scanning calorimetry, and 1 H nuclear magnetic resonance spectroscopy examinations. Carbon sources turned out to be critical for PHA production in AAPB. Among the eight media tested with Dinoroseobacter sp. JL1447, sodium acetate, giving a PHA production rate of 72%, was the most productive carbon source, followed by glucose, with a 68% PHA production rate. Such PHA production rates are among the highest recorded for all bacteria. The C/N ratio of substrates was verified by the experiments as another key factor in PHA production. In the case of R. denitrificans OCh 114, PHA was not detected when the organism was cultured at C/N ratios of <2 but became apparent at C/N ratios of >3. Light is also important for the formation of PHA in AAPB. In the case of Dinoroseobacter sp. JL1447, up to a one-quarter increase in PHB production was observed when the culture underwent growth in a light-dark cycle compared to growth completely in the dark.

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“…Lutaonella, the most closely associated organisms to OTU-2, are yellow-pigmented, Gramnegative, aerobic, non-motile, moderately thermophilic, rod-shaped bacteria; PHA granules have been observed after staining these cells with Sudan black (Arun et al 2009). Zoogloea showed higher relative abundances in S3 (with acetic acid-only feed) at the end of the accumulation whereas there was an opposite pattern in all the other sequences.…”

Section: Effect Of Enrichment Biomass Ecology On Pha Accumulation 734mentioning

confidence: 99%

Composition of mixed culture PHA biopolymers and implications for downstream processing

Montano-Herrera¹

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Polyhydroxyalkanoates (PHAs) are biopolymers synthesised and accumulated by most bacteria for carbon and energy storage. They have properties and applications comparable to petrochemical thermoplastics. Although PHAs are produced at high yields using pure biological cultures, the use of mixed cultures can significantly reduce the production costs and make use of waste streams to produce environmentally sustainable materials. In order to produce mixed culture PHAs of relevance for broader industry applications it is necessary to develop the ability to tailor polymers with diversified mechanical properties through understanding and controlling the monomer composition and compositional distribution.Diverse and complex PHA polymer structures have been achieved in mixed microbial cultures using time-based feeding strategies. However, PHA monomer composition and compositional distribution in PHA random and blocky copolymers are sensitive to substrate feeding history, making more complex the prediction of final PHA content and composition.In this sense, a better understanding of the changing cell physiologies that develop in response to different feeding strategies and substrates is necessary to design optimised feeding strategies and process control algorithms for PHA production.This thesis presents for the first time a comprehensive flux characterisation of monomer development during mixed culture PHA accumulation concurrent with biomass growth using Metabolic Flux Analysis (MFA). A dynamic trend in active biomass growth and in polymer composition was observed and was consistent over replicate accumulations. Monomer (3-hydroxybutyrate and 3-hydroxyvalerate) incorporation into poly(3-hydroxybutyate-co-3-hydroxyvalerate) copolymers in a pilot scale production system was evaluated based on published models that describe polymer production during PHA accumulation. However, it was found that these existing models could not describe the fluctuations in the proportion of Additionally, thermal degradation of mixed culture PHAs during melt processing was assessed by Near-Infrared (NIR) spectroscopy coupled to Multivariate Data Analysis. It was shown that, with correct pretreatment, a copolymeric product that was much more stable to extrusion processing than commercially available PHA was produced.Overall, this thesis explores both the fundamental and applied aspects of PHA production by mixed microbial cultures with concurrent active biomass growth. The use of computational iii tools to explore and help understand the underlying metabolic processes was explored.Polymer composition seems to follow a very complex regulation processes which can be described through the incorporation of more detailed reactions in current metabolic models.Furthermore, this thesis gives further insight into the fundamental properties of the materials produced and an assessment of their potential for degradation during processing.iv

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Microbial production of poly-β-hydroxybutyrate by marine microbes isolated from various marine environments

Cited by 63 publications

References 17 publications

Extraction and Characterization of Polyhydroxybutyrates (PHB) from Bacillus thuringiensisKSADL127 Isolated from Mangrove Environments of Saudi Arabia

Extraction and Characterization of Polyhydroxybutyrates (PHB) from Bacillus thuringiensisKSADL127 Isolated from Mangrove Environments of Saudi Arabia

Formation of Polyhydroxyalkanoate in Aerobic Anoxygenic Phototrophic Bacteria and Its Relationship to Carbon Source and Light Availability

Composition of mixed culture PHA biopolymers and implications for downstream processing

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