Microbial Production of d-Ribose

Sasajima, Ken-ichi; Yoneda, Masahiko

doi:10.1007/978-94-009-1111-6_11

Cited by 7 publications

(2 citation statements)

References 134 publications

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“…In the regression equation, the test variables were coded according to the equation: (2) where x i is the dimensionless value of an independent variable, X i is the real value of an independent variable, X 0 is the value of X i at the center point and ∆X is the step change.…”

Section: -3 Central Composite Design and Response Surface Methodologymentioning

confidence: 99%

“…D-ribose has been used for many decades to produce flavor enhancers (5 ' -nucleotides), riboflavin (vitamin B 2 ) [2], pharmaceuticals [3], cosmetics, food, animal feed additives [4], and antiviral and anticancer products [5,6]. Clinically, it has also been used to improve cardiac function and as a cure for cardiac ischemia and rare genetic diseases [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Optimization of medium components for D-ribose production by transketolase-deficient Bacillus subtilis NJT-1507

Fang

Chen

et al. 2010

Korean J. Chem. Eng.

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Statistical experimental designs were used to optimize the composition of culture media for the production of D-ribose by Bacillus subtilis. A fractional factorial design 2 (5-2) was used to determine medium components that significantly affected D-ribose production. The concentrations of glucose and (NH 4 ) 2 SO 4 were the significant factors. Central composite design and response surface methodology were then used to estimate the quadratic response surface and determine the factor levels for maximum production of D-ribose. Finally, the optimal medium composition was obtained (g/L): glucose, 172.75; (NH 4 ) 2 SO 4 , 13.2; yeast powder, 4; corn steep liquor, 8 and MnSO 4 , 0.5. This optimization strategy increased D-ribose production from 73.21 g/L to 88.57 g/L, an increase of 22% compared with the original conditions. The D-ribose production yield to glucose concentration was also enhanced from 0.37 g/g to 0.52 g/g. Confirmatory experiments were also performed to demonstrate the accuracy of the model. Under the optimal medium using ammonia to control pH in a 5 L fermenter, the D-ribose yield was increased to 95.28 g/L after 3 days of cultivation at 37 o C.

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Section: -3 Central Composite Design and Response Surface Methodologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of medium components for D-ribose production by transketolase-deficient Bacillus subtilis NJT-1507

Fang

Chen

et al. 2010

Korean J. Chem. Eng.

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Megacell phenotype and its relation to metabolic alterations in transketolase deficient strain of Bacillus pumilus

Srivastava

Jaiswal

Panda

et al. 2008

Biotech & Bioengineering

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Fermentation with transketolase (tkt) deficient strain of Bacillus is the only reported industrially viable process for production of D-ribose, a commercially important pentose sugar. In addition to direct effects of tkt deficiency, the mutation in non-oxidative part of pentose phosphate pathway (PPP) is known to display several unexpected physiological characteristics such as decreased ability to utilize D-glucose, altered carbon catabolite repression, lack of motility, etc. Here we demonstrate the morphological plasticity of tkt deficient strain of Bacillus pumilus ATCC 21951 and its possible relation with D-ribose productivity, a measure of carbon flux through PPP. The bacilli divide normally in nutrient rich media such as Luria-Bertani (LB) broth while showing cell elongation of up to 20-fold without a visible septum accompanied by moderate to high extracellular D-ribose accumulation in glucose-rich media. The cells stained with DAPI (4'-6-diamidino-2-phenylindole) and anti FtsZ antibody showed nucleoid separation and Z-ring formation in LB broth but not in glucose-rich media. FtsZ protein is known to localize at the future division site forming a ring, called Z-ring, at an early stage in cytokinesis. The strain experiences inhibition or delay in Z-ring formation resulting in cell elongation, possibly due to its altered cell membrane composition resulting from tkt deficiency. We hypothesize that the lack of PPP intermediates may have two effects on the strain: (i) altered the cell membrane leading to delay in Z-ring formation and cell elongation; and (ii) induction of genes of the oxidative part of PPP resulting in D-ribose accumulation. Nutrient rich media such as LB broth may alleviate these metabolite deficiencies thereby restoring normal cell division and inhibiting excessive D-ribose accumulation. The D-ribose productivity and cell elongation may therefore be co-morbid. The results have implications in designing optimal media and monitoring strategy based on morphological analysis.

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d-Glucose does not catabolite repress a transketolase-deficientd-ribose-producingBacillus subtilis mutant strain

Wulf

Soetaert

Schwengers

et al. 1996

Journal of Industrial Microbiology

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WhenBacillus subtilis strain ATCC 21951, a transketolase-deficient D-ribose-producing mutant, was grown on Dglucose plus a second substrate which is metabolized via the oxidative pentose phosphate cycle (D-gluconic acid, D-xylose, L-arabinose or D-xylitoI), D-glucose did not catabolite repress metabolism of the second carbon source. The D-ribose yield obtained with the simultaneously converted carbon substrates, significantly exceeded that when only D-glucose was used. In addition, the concentration of glycolytic by-products and the fermentation time significantly decreased. Based on these findings, a fermentation process was developed with B. subtilis strain ATCC 21951 in which D-glucose (100 g L 1) and D-gluconic acid (50 g L -1) were converted into 45 g L -1 of D-ribose and 7.5 g L -1 of acetoin. A second process, based on D-glucose and D-xylose (100 g L -1 each), yielded 60 g L -1 of e-ribose and 4 g L -1 of acetoin plus 2,3-butanediol. Both mixed carbon source fermentations provide excellent alternatives to the less efficient D-glucose-based processes used so far.

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Microbial Production of d-Ribose

Cited by 7 publications

References 134 publications

Optimization of medium components for D-ribose production by transketolase-deficient Bacillus subtilis NJT-1507

Optimization of medium components for D-ribose production by transketolase-deficient Bacillus subtilis NJT-1507

Megacell phenotype and its relation to metabolic alterations in transketolase deficient strain of Bacillus pumilus

d-Glucose does not catabolite repress a transketolase-deficientd-ribose-producingBacillus subtilis mutant strain

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