Microbial production of C2-hydrocarbons, ethane, ethylene and acetylene.

Fukuda, Hideo; Fujii, Tomoyuki; Ogawa, Takahira

doi:10.1271/bbb1961.48.1363

Cited by 28 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Supposing both methods (nutrient broth and agar plate) produce comparable bacterial numbers and both media support acetylene production equally strong, we should have observed C 2 H 2 concentrations of tens of ppbv. Thus, we are unable to reproduce the result obtained in [7], although the difference in the growth media should be emphasized. Figure 2 shows a sample of a real-time measurement of the acetylene mixing ratio in exhaled breath after cigarette smoking.…”

Section: Background Level and Bacterial Measurementsmentioning

confidence: 77%

“…As one cigarette has been found to release about 150 μg of acetylene to the environment [6], it seems reasonable to assume that smokers exhibit higher concentrations of C 2 H 2 in breath than non-smokers. Some bacteria have also been shown to produce small hydrocarbons like acetylene [7], and thus it is also possible that C 2 H 2 is endogenously produced in the human body.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Acetylene in breath: background levels and real-time elimination kinetics after smoking

et al. 2010

View full text Add to dashboard Cite

We have measured the acetylene concentration in the exhaled breath of 40 volunteers (31 non-smokers, nine smokers) using near-infrared cavity ring-down spectroscopy. The acetylene levels were found to be the same as in ambient air for non-smokers, whereas elevated levels were observed for smokers. Real-time measurements with sub-second time resolution have been applied to measure the elimination kinetics of acetylene in breath after exposure to tobacco smoke. Three exponential time constants can be distinguished from the data and these can be used to define the residence times for different compartments, according to the multi-compartment model of the human body.

show abstract

Section: Background Level and Bacterial Measurementsmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Acetylene in breath: background levels and real-time elimination kinetics after smoking

et al. 2010

View full text Add to dashboard Cite

show abstract

“…at 30 "C for 1 h. After incubation, a sample of gas (1 ml) was withdrawn through the stopper with a gas syringe and the amount of ethylene in the sample was determined with a gas chromatograph (G3800; Yanako) under the following conditions: column size, 3.mm x 2 m; solid phase, active alumina; temperature, 100 "C; carrier gas, nitrogen (flow rate 40 ml min-l); detection, flame ionization. The rate of ethylene formation was calculated as previously described (Fukuda et al, 1984).…”

Section: Methodsmentioning

confidence: 99%

l-Arginine is essential for the formation in vitro of ethylene by an extract of Pseudomonas syringae

Nagahama

Ogawa

Fujii

et al. 1991

Journal of General Microbiology

View full text Add to dashboard Cite

A system was developed for the formation of ethylene in vitro by an extract of Pseudomonas syringae pv. phaseolicola PK2. The ethylene-forming activity of a cell-free extract of this bacterium measured in a system reported previously was almost completely lost when the cell-free extract was dialysed against potassium phosphate buffer for 24 h at 4 "C. When the fraction of cell-free extract with a molecular mass < 10 kDa (SupI) was added back to the enzyme fraction after gel filtration of the cell-free extract, the enzymic activity increased to about four times that of the gel-filtered crude enzyme. The action of SupI could be reproduced by the addition of L-arginine. The complete system for the formation of ethylene under aerobic conditions in vitro required 0.25 mM-2-oxoglutarate, 0.2 mM-FeSO,, 2 mM-Dn, 10 mM-L-histidine and 0.2 mM-L-arginine. The cofactor specificity was examined by replacing L-arginine or L-histidine with various analogues, but none of them were effective. The components of this system, with the exception of L-histidine, were similar to those of a system derived from the ethylene-producing, plant pathogenic fungus Penicillium digitatum which also produced ethylene in vitro in a reaction dependent on 2-oxoglutarate. The intermediates in the ethylene-forming reaction are postulated and the roles of L-arginine and L-histidine in the formation of ethylene by Ps. syringae are discussed.

show abstract

“…Infection by pathogens often leads to an increased ethylene production by host plants (e.g. Pegg, 1976;Boller, 1991), and some pathogens have themselves been found to produce ethylene (Fukuda et al, 1984;Arshad and Frankenberger, 1991). Ethylene production is elevated in the above-ground portions of flooded plants (Hunt et al,1981;Voesenek et al, 1990;Rijnders et al, 1992).…”

mentioning

confidence: 99%

Ethylene and Not Embolism Is Required for Wound-Induced Tylose Development in Stems of Grapevines

Sun¹,

Rost²,

Reid³

et al. 2007

Plant Physiol.

View full text Add to dashboard Cite

The pruning of actively growing grapevines (Vitis vinifera) resulted in xylem vessel embolisms and a stimulation of tylose formation in the vessels below the pruning wound. Pruning was also followed by a 10-fold increase in the concentration of ethylene at the cut surface. When the pruning cut was made under water and maintained in water, embolisms were prevented, but there was no reduction in the formation of tyloses or the accumulation of ethylene. Treatment of the stems with inhibitors of ethylene biosynthesis (aminoethoxyvinylglycine) and/or action (silver thiosulfate) delayed and greatly reduced the formation of tyloses in xylem tissue and the size and number of those that formed in individual vessels. Our data are consistent with the hypotheses that wound ethylene production is the cause of tylose formation and that embolisms in vessels are not directly required for wound-induced tylosis in pruned grapevines. The possible role of ethylene in the formation of tyloses in response to other stresses and during development, maturation, and senescence is discussed.

show abstract

Microbial production of C2-hydrocarbons, ethane, ethylene and acetylene.

Abstract: A series of hydrocarbons having two carbon atoms (C2-hydrocarbons) such as ethane,

Cited by 28 publications

References 0 publications

Acetylene in breath: background levels and real-time elimination kinetics after smoking

Acetylene in breath: background levels and real-time elimination kinetics after smoking

l-Arginine is essential for the formation in vitro of ethylene by an extract of Pseudomonas syringae

Ethylene and Not Embolism Is Required for Wound-Induced Tylose Development in Stems of Grapevines

Contact Info

Product

Resources

About