Microbial production of advanced biofuels

Keasling, Jay D.; Martín, Héctor García; Lee, Taek Soon; Mukhopadhyay, Aindrila; Singer, Steven W.; Sundström, Eric

doi:10.1038/s41579-021-00577-w

Cited by 173 publications

(89 citation statements)

References 318 publications

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“…Our observation that pAF production trades off with growth in E. coli illustrates the disruption of cellular homeostasis caused by the expression of heterologous enzymatic pathways, a phenomenon observed in other related studies [21, 37-39]. This motivates the need to engineer the host cell’s native metabolism in order to optimize nsAA production in dynamic environments.…”

Section: Resultssupporting

confidence: 72%

Computational design and construction of an Escherichia coli strain engineered to produce a non-standard amino acid

Zomorrodi

Hemez

Arranz‐Gibert

et al. 2022

Preprint

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Introducing heterologous pathways into host cells constitutes a promising strategy for synthesizing nonstandard amino acids (nsAAs) to enable the production of proteins with expanded chemistries. However, this strategy has proven challenging as the expression of heterologous pathways can disrupt cellular homeostasis of the host cell. Here, we sought to optimize the heterologous production of the nsAA para-aminophenylalanine (pAF) in Escherichia coli. First, we incorporated a heterologous pAF biosynthesis pathway into a genome-scale model of E. coli metabolism, and computationally identified metabolic interventions in the host's native metabolism to improve pAF production. Next, we explored different ways of imposing these flux interventions experimentally and found that the upregulation of flux in chorismate biosynthesis pathway through the elimination of feedback inhibition mechanisms could significantly raise pAF titers (~20 fold) while maintaining a reasonable pAF yield-growth rate trade-off. Overall, this study provides a promising strategy for the biosynthesis of nsAAs in engineered cells.

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Section: Resultssupporting

confidence: 72%

Computational design and construction of an Escherichia coli strain engineered to produce a non-standard amino acid

Zomorrodi

Hemez

Arranz‐Gibert

et al. 2022

Preprint

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“…These changes in the social and economic paradigm and the rapid development of engineering and analytical techniques have promoted the application of microbial metabolic engineering to diverse fields, such as food, pharmaceutical, cosmetic, biochemical, and biofuel industries. The reports of successful commercial microbial processes for the production of biofuels, natural or non-natural chemicals, and pharmaceuticals have been published consistently [ 1 , 2 ]. The range of products obtained via microbial processes can be expanded by merging synthetic biology and bioinformatics with microbial metabolic engineering [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Organelle Engineering in Yeast: Enhanced Production of Protopanaxadiol through Manipulation of Peroxisome Proliferation in Saccharomyces cerevisiae

et al. 2022

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Isoprenoids, which are natural compounds with diverse structures, possess several biological activities that are beneficial to humans. A major consideration in isoprenoid production in microbial hosts is that the accumulation of biosynthesized isoprenoid within intracellular membranes may impede balanced cell growth, which may consequently reduce the desired yield of the target isoprenoid. As a strategy to overcome this suggested limitation, we selected peroxisome membranes as depots for the additional storage of biosynthesized isoprenoids to facilitate increased isoprenoid production in Saccharomyces cerevisiae. To maximize the peroxisome membrane storage capacity of S.cerevisiae, the copy number and size of peroxisomes were increased through genetic engineering of the expression of three peroxisome biogenesis-related peroxins (Pex11p, Pex34p, and Atg36p). The genetically enlarged and high copied peroxisomes in S.cerevisiae were stably maintained under a bioreactor fermentation condition. The peroxisome-engineered S.cerevisiae strains were then utilized as host strains for metabolic engineering of heterologous protopanaxadiol pathway. The yields of protopanaxadiol from the engineered peroxisome strains were ca 78% higher than those of the parent strain, which strongly supports the rationale for harnessing the storage capacity of the peroxisome membrane to accommodate the biosynthesized compounds. Consequently, this study presents in-depth knowledge on peroxisome biogenesis engineering in S.cerevisiae and could serve as basic information for improvement in ginsenosides production and as a potential platform to be utilized for other isoprenoids.

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“…They synthesize biodiesel as part of their metabolism. In addition, transesterification is employed (Keasling et al 2021 ). Thus, identifying best suited microalgae, making them genetically accessible and further improving their biodiesel production rates are the most urgent goals.…”

Section: The Future Challengesmentioning

confidence: 99%

Microorganisms harbor keys to a circular bioeconomy making them useful tools in fighting plastic pollution and rising CO2 levels

Antranikian

Streit

2022

Extremophiles

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The major global and man-made challenges of our time are the fossil fuel-driven climate change a global plastic pollution and rapidly emerging plant, human and animal infections. To meet the necessary global changes, a dramatic transformation must take place in science and society. This transformation will involve very intense and forward oriented industrial and basic research strongly focusing on (bio)technology and industrial bioprocesses developments towards engineering a zero-carbon sustainable bioeconomy. Within this transition microorganisms—and especially extremophiles—will play a significant and global role as technology drivers. They harbor the keys and blueprints to a sustainable biotechnology in their genomes. Within this article, we outline urgent and important areas of microbial research and technology advancements and that will ultimately make major contributions during the transition from a linear towards a circular bioeconomy.

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Microbial production of advanced biofuels

Cited by 173 publications

References 318 publications

Computational design and construction of an Escherichia coli strain engineered to produce a non-standard amino acid

Computational design and construction of an Escherichia coli strain engineered to produce a non-standard amino acid

Organelle Engineering in Yeast: Enhanced Production of Protopanaxadiol through Manipulation of Peroxisome Proliferation in Saccharomyces cerevisiae

Microorganisms harbor keys to a circular bioeconomy making them useful tools in fighting plastic pollution and rising CO2 levels

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