Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30

Zhang, Liaoyuan; Yang, Yang; Sun, Jianan; Shen, Yang; Wei, Dongzhi; Zhu, Junwen; Chu, Ju

doi:10.1016/j.biortech.2009.10.052

Cited by 127 publications

(97 citation statements)

References 22 publications

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“…Sucrose occupied a weight fraction of over 86% of the total fermentable sugar. As reported previously, the strain of S. marcescens H30 could efficiently utilize high concentration sucrose for 2,3-BD production achieving high yield (Zhang et al 2010a). Therefore, the SSJ with high sugar concentration has the potential for 2,3-BD production by this strain.…”

Section: Chemical Composition Of the Ssjsupporting

confidence: 51%

“…The optimum nitrogen source was used to carry out further experiments. According to the results from previous studies (Zhang et al 2010a;Sun et al 2012), several inorganic salts (sodium acetate 0-10 g/L, KH2PO4 0-1 g/L, MgSO4 0-0.5 g/L, MnSO4 0-0.5 g/L, FeSO4 0-0.5 g/L, and ZnCl2 0-0.5 g/L) were used to investigate the effects on 2,3-BD production by S. marcescens H30. All the experiments using various inorganic salts were carried out in 50 mL fresh medium containing the SSJ content of 75% and 5 g/L yeast extract for 36 h at 150 rpm and 30 °C in triplicate.…”

Section: Effect Of Nitrogen Sources and Inorganic Salts On 23-bd Promentioning

confidence: 95%

“…The biomass concentration was determined by the optical density at the wavelength of 600 (OD600) in a spectrophotometer (UV-1800, Mapada, China) and correlated with dry cell weight (DCW) as described previously (Zhang et al 2010a). The linear equation is DCW (g/L) = 0.422 * OD600 -0.0363.…”

Section: Methodsmentioning

confidence: 99%

“…The seed preparation was conducted at 30 °C with shaking at 180 rpm for 12 h in a seed medium containing 10 g/L of glucose, 2 g/L of peptone, 1 g/L of yeast extract, 6 g/L of (NH4)2SO4, 10 g/L of KH2PO4, 0.5 g/L of NaCl, and 0.5 g/L of MgSO4 at pH 7.2 (Zhang et al 2010a). …”

Section: Microorganism and Seed Preparationmentioning

confidence: 99%

“…Some microbial fermentation processes have been developed to enhance the 2,3-BD production in recent years. High 2,3-BD concentrations and productivities have been achieved from pure carbon sources by use of several microbial strains such as Klebsiella pneumoniae, Serratia marcescens, and Bacillus licheniformis (Ma et al 2009;Zhang et al 2010a;Jurchescu et al 2013).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Yuan¹,

He²,

Guo³

et al. 2017

BioResources

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Sweet sorghum juice (SSJ) is considered a good carbon source for biorefinery due to its low price and high fermentable-sugar content. In this study, 2,3-butanediol (2,3-BD) production from SSJ by Serratia marcescens H30 was investigated. First, the medium compositions including the contents of SSJ, nitrogen source, and mineral salts were optimized in conical flasks using a single factor and orthogonal design method. Under the optimal conditions, the 2,3-BD concentration reached up to 33.40 g/L. Then the optimized medium was used to perform fermentative experiments in a 5-L bioreactor. In batch experiments, the effects of various agitation speeds on 2,3-BD production were compared. Based on batch process results, an efficient two-stage fermentative control strategy was developed, where the agitation speed was maintained at 300 rpm in the first 12 h and subsequently switched to 200 rpm. About 43.32 g/L 2,3-BD was obtained by using this strategy. Finally, fed-batch fermentation was conducted through feeding the concentrated SSJ and a maximum 2,3-BD concentration of 109.44 g/L with the productivity of 1.40 g/L·h; a yield of 83.02% was achieved. The results showed that SSJ could be used as an economical substrate for efficient 2,3-BD production by S. marcescens H30.

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Section: Chemical Composition Of the Ssjsupporting

confidence: 51%

Section: Effect Of Nitrogen Sources and Inorganic Salts On 23-bd Promentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Microorganism and Seed Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Yuan¹,

He²,

Guo³

et al. 2017

BioResources

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Bio‐Based Butanediols Production: The Contributions of Catalysis, Metabolic Engineering, and Synthetic Biology

Huang

2014

Bioprocessing of Renewable Resources to Commodity Bioproducts

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Optimization of 2,3‐butanediol production by Enterobacter cloacae in simultaneous saccharification and fermentation of corncob residue

Zhang

Peng

et al. 2014

Biotech and App Biochem

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Corncob residue, a waste in xylose or xylitol production, was utilized to produce 2,3-butanediol (2,3-BD) via simultaneous saccharification and fermentation (SSF). This study developed the optimal conditions for production of 2,3-BD by using a heat-resistant strain, Enterobacter cloacae UV4, to perform SSF of the corncob residue. Urea, lactic acid, sodium citrate, and MgSO4 , selected by the Plackett-Burman experiment, were determined to be significant independent variables to conduct the response surface experiment. With the optimized medium, a total production of 28.923 g/L for 2,3-BD and acetoin (BA) was obtained at 60 H. Furthermore, 43.162 g/L of BA production and 0.553 g/L/H of productivity were obtained by fed-batch SSF, which was 0.424 g diol/g consumed corncob residue. The results suggest that the waste corncob residue could be used as an available substrate for the production of 2,3-BD by E. cloacae UV4, as well as a potential resource to improve the economics of microbial compound production.

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Microbial production of 2,3-butanediol by a mutagenized strain of Serratia marcescens H30

Cited by 127 publications

References 22 publications

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Bio‐Based Butanediols Production: The Contributions of Catalysis, Metabolic Engineering, and Synthetic Biology

Optimization of 2,3‐butanediol production by Enterobacter cloacae in simultaneous saccharification and fermentation of corncob residue

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