Microbial potentiometric sensor: A new approach to longstanding challenges

Burge, Scott R.; Hristovski, Kiril; Burge, Russell G.; Hoffman, David; Saboe, Daniel; Chao, Peng Fei; Taylor, Evan; Koenigsberg, Stephen S.

doi:10.1016/j.scitotenv.2020.140528

Cited by 24 publications

(9 citation statements)

References 27 publications

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“…In this context, biosensors have emerged as an efficient tool for the fast, sensitive, and selective determination of toxins directly. 141 In a biosensor, on the basis of the metabolic activity level of the biocatalyst (microorganism or enzyme), the response signal (current) varies. Hence, the toxicity can be determined by monitoring the influence on the activity variation.…”

Section: Inhibitionmentioning

confidence: 99%

Tuning of Electrode Surface for Enhanced Bacterial Adhesion and Reactions: A Review on Recent Approaches

Ratheesh

Elias

Shibli

2021

ACS Appl. Bio Mater.

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The study of bacterial adhesion and its consequences has great significance in different fields such as marine science, renewable energy sectors, soil and plant ecology, food industry, and the biomedical field. Generally, the adverse effects of microbial surface interactions have attained wide visibility. However, herein, we present distinct approaches to highlight the beneficial aspects of microbial surface interactions for various applications rather than deal with the conventional negative aspects or prevention strategies. The surface microbial reactions can be tuned for useful biochemical or bio-electrochemical applications, which are otherwise unattainable through conventional routes. In this context, the present review is a comprehensive approach to highlight the basic principles and signature parameters that are responsible for the useful microbial−electrode interactions. It also proposes various surface tuning strategies, which are useful for tuning the electrode characteristics particularly suitable for the enhanced bacterial adhesion and reactions. The tuning of surface characteristics of electrodes is discussed with a special reference to the Microbial Fuel Cell as an example.

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Section: Inhibitionmentioning

confidence: 99%

Tuning of Electrode Surface for Enhanced Bacterial Adhesion and Reactions: A Review on Recent Approaches

Ratheesh

Elias

Shibli

2021

ACS Appl. Bio Mater.

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“…This anaerobic solution was introduced into the reactor and allowed to equilibrate for a period of more than two months to ensure the population of an endemic biofilm onto the surface of the three MPS graphite electrodes. Two weeks of equilibration typically yield biofilm growth suitable for MPS measurements [18]. During the biofilm cultivation period, the solution was continuously stirred and the experimental chamber was located in a dark room, to prevent the growth of photosynthetic microorganisms, and held at temperature of 23 ± 1°C.…”

Section: Batch Reactor Fabrication and Cultivation Of The Biofilm On mentioning

confidence: 99%

“…The reasoning behind this type of signal area integration was based on the behavior of the microorganisms in the biofilm upon exposure to toxic chemicals. When toxins are introduced, the MPS signal rapidly decreases and correlates to the inhibition of the metabolic (e.g., electron generation/cytochrome storage) processes [18]. Once toxic metals are immobilized, or otherwise removed from the environment, the inhibitory effects are eliminated, and normal metabolic activities resume, which can be observed by a characteristic increase in the microbial OCP signal (recovery portion of the signal).…”

Section: Examining the Microbial Potentiometric Sensor Cumulative Resmentioning

confidence: 99%

“…A recent study by Burge et al [18] demonstrated a novel type of microbial potentiometric sensor (MPS) capable of detecting changes in the local aquatic environment surrounding the biofilmpopulated sensing electrodes. Considering that toxic metals can affect metabolic processes in microorganisms adversely, it is reasonable to hypothesize that these metals in water matrices would induce a decrease in metabolic activity of the biofilm microorganisms populating the surface of the sensing electrode.…”

Section: Introductionmentioning

confidence: 99%

“…Considering that toxic metals can affect metabolic processes in microorganisms adversely, it is reasonable to hypothesize that these metals in water matrices would induce a decrease in metabolic activity of the biofilm microorganisms populating the surface of the sensing electrode. This effect could be registered as a change in the open-circuit potential (OCP) generated by the biofilm, as described by Burge et al (2020). The typical MPS response, the OCP of the biofilm versus Ag/AgCl electrode, ranges from approximately -700 to +800 mV, depending on the system and the biochemical conditions.…”

Section: Introductionmentioning

confidence: 99%

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Microbial potentiometric sensor technology for real-time detecting and monitoring of toxic metals in aquatic matrices

Brown

Burge

Hristovski

et al. 2020

Maced. J. Chem. Chem. Eng.

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Considering that toxic metals can affect metabolic processes in microorganisms adversely, it can be hypothesized that these metals in water matrices would induce a decrease in metabolic activity of the biofilm microorganisms populating the surface of a sensing electrode, which could be registered as a change in the open-circuit potential (OCP) generated by the biofilm microorganisms. The goal of this study was to test this hypothesis and demonstrate the underlying principle that microbial potentiometric sensor (MPS) technology could be used for long-term and real-time monitoring and detection of rapid changes in metal concentrations in realistic aquatic environments. To address the goal, four objective were addressed: (1) a batch reactor with three graphite-based MPS electrodes was fabricated; (2) a set of single-ion solutions and one multiple ion solution were prepared reflecting realistic concentrations of metals found in electroplating wastewaters; (3) the responses of the MPS to the simultaneous presence of multiple toxic metal ions in a single solution were measured; and (4) the changes of the MPS signals to the presence of individual metal ion solutions were examined. While the hypothesis was validated, the study also revealed that the MPS was sufficiently sensitive to not only detect, but also quantify, toxic metal ion concentrations in aqueous solutions. The coefficients of determination, which were R2>0.995, and responsiveness of <1 μmol/L for some toxic metal cations, strongly support the performance of MPS technology in the echelons of expensive analytical tools capable detecting and measuring trace elements.The magnitude of the MPS response was toxic metal specific. When the molar concertation normalizes the inhibition portion of the signal area, the assessed sensitivity order was: Se>Cd>Pb>Ag>Ni> Zn. The study provides valuable information for enforcement agents, environmental professionals, and wastewater treatment operators, so toxic metal pollution and its detrimental impacts can be prevented and mitigated.

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Status and future trends in wastewater management strategies using artificial intelligence and machine learning techniques

Baskar,

Nashath Omer,

Saravanan

et al. 2024

Chemosphere

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Microbial potentiometric sensor: A new approach to longstanding challenges

Cited by 24 publications

References 27 publications

Tuning of Electrode Surface for Enhanced Bacterial Adhesion and Reactions: A Review on Recent Approaches

Tuning of Electrode Surface for Enhanced Bacterial Adhesion and Reactions: A Review on Recent Approaches

Microbial potentiometric sensor technology for real-time detecting and monitoring of toxic metals in aquatic matrices

Status and future trends in wastewater management strategies using artificial intelligence and machine learning techniques

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