Microbial Populations During Composting Process of Organic Fraction of Municipal Solid Waste

Rebollido, R.; Martínez, Josefina; Aguilera, Yolanda; Melchor, K.; Koerner, I.; Stegmann, R.

doi:10.15666/aeer/0603_061067

Cited by 54 publications

(42 citation statements)

References 15 publications

(10 reference statements)

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“…A thermometer was placed into compost pile to determine its temperature and after several minutes, the compost temperature was recorded and the compost thermometer was also used to measure the areas surrounding the compost pile [15]. The moisture content of the compost samples determined in ovens at 105˚C for 24 -48 hours and the slurry with a ratio of 1:10 was obtained from the compost samples and then the pH measurement was done using pH meter [3]. To determine the C/N ratio, total carbon content of the samples determined through combustion in ovens at 750˚C for 2 hours and total nitrogen analysis was performed by Kjeldahl method [16].…”

Section: Methodsmentioning

confidence: 99%

“…primidone and thiamine) to grow fungi [2]. During a compost process which is an aerobic process, the organic matter of waste can be converted into useful organic manure by microorganisms [3]. This widespread technology destroys pathogens and converts potentially harmful waste products into an odorless and inoffensive material and it may also have more positive effects on the physical, chemical and biological characteristics of soil by increasing vegetation, a protective layer to prevent soil erosion [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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Identification of Fungal Communities in Producing Compost by Windrow Method

Dehghani¹,

Asadi²,

Charkhloo³

et al. 2012

JEP

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Considering the importance of fungal genera in producing compost from piling organic matter or biodegradable waste, this study aimed to identify the fungal species and their negative effect on human health during the compost production by windrow method. In this descriptive study, 99 compost samples were collected from the composting research site at Kashan University of medical sciences within 63 days from Oct 3 to Dec 4, 2010. Sixty-six samples were cultured to determine the fungal species and 33 samples were transferred to the laboratory to determine the physical and chemical parameters. Twenty-five fungal species were identified during the compost process including 15 saprophytes, 8 dermatophites, 1 opportunistic and 1 yeast fungi. Identified fungal species during the compost process according to their frequencies were Aspergillus spp (34.45%), Microsporium spp (18.89%), Trichophyton spp (8.89%), Yeast sp (6.67%), Mucor sp (5.56%), Penicillium sp (4.45%), Rhizopus sp (4.45%), Fusarium sp (3.34%), Cladosporium sp (3.34%), Curvularia sp (3.34%) and also other fungal species (6.62%), respectively. Considering the presence of various pathogenic fungi during the composting process, the employees of the compost factory should use individual protective devices and also due to the presence of 11 fungal species in the mature compost and their pathogenic effect, preventive measures should be taken by persons involved in the production of compost especially farmers, in order to prevent successive inhalation of spores.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Fungal Communities in Producing Compost by Windrow Method

Dehghani¹,

Asadi²,

Charkhloo³

et al. 2012

JEP

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“…As composting is a biological aerobic decomposition process consisted from different phases, microbial groups concerned with this process have intensively been investigated by using a variety of culture-based [2]- [5] and unculture-based techniques [6]- [14]. Because application of organic fertilizer, prepared under suitable composting process, has found to maintain soil fertility by amending physical, chemical and biological properties of field soil.…”

Section: Introductionmentioning

confidence: 99%

“…Although traditional culture based approach such as dilution plate count can clarify change of microbial population during the process, culture-based isolate is not a guarantee of the numerically dominant microorganism and its contribution remains unclear [2]- [5]. Although unculture-based technique, such as denaturing gradient gel electrophoresis (DGGE) [7] [8] [10] [11] [13] or clone library sequencing [6] [9] [11] [12] [14], targets the both cultured and uncultured microorganisms, the method only reveals variations of relative abundance of each microbial groups and a change of the microbial numbers remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Groups Concerned with Maturing Process in Manure Production Analyzed by a Method Based on Restriction Fragment Length Polymorphism Analysis

Watanabe¹,

Horinishi²,

Matumoto³

et al. 2015

AiM

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Composting is a biological aerobic decomposition process consisted from different phases. Although the Japanese Standards for manure recommended that it took at least 6 months to complete the maturing phase, there was no reliable ground. In order to find out shortening method of the maturing phase, the microorganisms concerned with a progress of the maturing was determined by using the most probable number method (MPN) and PCR-RFLP of the 16S rDNA, which was found effective to provide numbers and taxonomy of polymyxin B resistant bacterial groups in the former paper [1]. Compared to the numbers after thermophilic phase, those of Actinobacteria, δ-proteobacteria, and the other gram negative bacteria increased to 50 times, 20 times, and 10 5 times, respectively, after maturing phase, while those of Bacillus spp., and α and β-proteobacteria decreased to 1/10, and 1/10 5 after maturing phase. Numbers of the other Fumicutes, and γ-proteobacteria remained in the same revel. Actinobacteria, δ-proteobacteria, and the other gram negative bacteria might be concerned with a progress of the maturing phase, because these bacterial groups were detected and enumerated due to their proliferation ability. Although number of Acitinobacteria might be underestimated because of a PCR bias, the method was found effective for the purpose to monitor bacteria actively proliferated in culture medium.

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“…A temperatura do processo de compostagem é o principal fator que determinará a sucessão das populações microbianas e sua representatividade nas fases de degradação, sendo elas a mesofílica e a termofílica (Rebollido et al, 2008). A temperatura também pode ser usada como referencial de indicação da evolução e qualidade do processo.…”

Section: Introductionunclassified

Temperatura de degradação de resíduos em processo de compostagem e qualidade microbiológica do composto final

Silva

Marco

Hahn

et al. 2013

Rev. bras. eng. agríc. ambient.

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RESUMOA técnica da compostagem é um recurso empregado com o objetivo de reciclar resíduos orgânicos domésticos e sanitizar o lodo produzido nas estações de tratamento de efluentes de esgoto (ETE) podendose obter um composto com propriedades de fertilizante agrícola e/ou corretor de solos degradados. A alta temperatura atingida pelo sistema deve ser responsável pela redução de micro-organismos patogênicos presentes no início do processo assegurando, desta forma, a qualidade microbiológica do composto sem oferecer riscos de contaminação, conforme preconiza a Resolução 354/2006 do CONAMA. Objetivouse, com o trabalho, avaliar a influência da temperatura sobre a redução de Escherichia coli, Salmonella sp., ovos de helmintos e vírus entéricos durante o processo de compostagem, além da contagem de bactérias heterotróficas. Foram realizados testes colimétricos, semeaduras em meios de cultivo, testes de visualização de ovos de helmintos e detecção molecular de vírus entéricos. Os resultados indicaram oscilação nas contagens de E. coli e de bactérias heterotróficas, mesmo após a fase termofílica. Por outro lado, não foi detectada a presença de Salmonella sp., vírus entéricos nem de ovos viáveis de helmintos, ao final do processo. Palavras-chave: lodo de esgoto, coliformes, matéria orgânicaEvaluation of degradation temperature of compounds in a composting process and microbiological quality of the compost ABSTRACT The composting technique is a promising alternative for the treatment of solid organic residues, along with the sludge produced in sewage treatment plants. The final compost might show fertilizer properties, which can be used to recover poor soils. The high temperatures that the composting process can achieve are responsible for the reduction of the pathogenic microbial population, helminth eggs and virus present in the beginning of the process. This behavior assures the microbial quality of the compost according to the 375/2006 Resolution of the CONAMA. The aim of this study was to evaluate the influence of temperature on Escherichia coli, Salmonella sp., helminth egg and enteric virus presence in the final product from a composting process. For this matter colimetric assays were performed together with the seedling of the samples in different culture media, the assay for helminth eggs visualization under the microscope and the identification of enteric virus. The results showed an oscillation on E. coli and heterotrophic bacteria counts, even after the thermophilic phase. On the other hand, Salmonella sp., helminth eggs and enteric virus were not found in the final compost.

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Microbial Populations During Composting Process of Organic Fraction of Municipal Solid Waste

Cited by 54 publications

References 15 publications

Identification of Fungal Communities in Producing Compost by Windrow Method

Identification of Fungal Communities in Producing Compost by Windrow Method

Bacterial Groups Concerned with Maturing Process in Manure Production Analyzed by a Method Based on Restriction Fragment Length Polymorphism Analysis

Temperatura de degradação de resíduos em processo de compostagem e qualidade microbiológica do composto final

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