Microbial physiological engineering increases the efficiency of microbial cell factories

Liu, Hui; Qi, Yanli; Zhou, Pei; Ye, Chao; Gao, Cong; Chen, Xiulai; Li, Liming

doi:10.1080/07388551.2020.1856770

Cited by 20 publications

(15 citation statements)

References 118 publications

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“…To establish this property, we first require the identification of stress-responsive functional and regulatory elements. Currently, the promising targets that can be used for improving the tolerance and robustness of industrial microorganisms include molecular chaperones, transporters, transcription regulators, cell membranes, macromolecule repair systems, and reactive oxygen species (ROS)-scavenging enzymes (Mukhopadhyay, 2015;Qi et al, 2019;Liu et al, 2021), which provide the foundation for strain improvement through synthetic biology. Frontiers in Bioengineering and Biotechnology frontiersin.org…”

Section: Discussionmentioning

confidence: 99%

“…In past decades, engineering the robustness of strains to industrially relevant stress factors has proven to be conducive to achieving higher yield and productivity ( Mukhopadhyay, 2015 ; Ko et al, 2020 ; Liu et al, 2021 ; Mohedano et al, 2022 ). However, the underlying mechanisms of how C. glutamicum tolerates high-lysine concentrations are still unclear, which obviously impedes the identification of promising targets for improving the robustness of cell factories.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum

Wang

Yang

Shi

et al. 2022

Front. Bioeng. Biotechnol.

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Corynebacterium glutamicum has been widely and effectively used for fermentative production of l-lysine on an industrial scale. However, high-level accumulation of end products inevitably leads to osmotic stress and hinders further increase of l-lysine production. At present, the underlying mechanism by which C. glutamicum cells adapt to high-lysine-induced osmotic stress is still unclear. In this study, we conducted a comparative transcriptomic analysis by RNA-seq to determine gene expression profiles under different high-lysine stress conditions. The results indicated that the increased expression of some metabolic pathways such as sulfur metabolism and specific amino acid biosynthesis might offer favorable benefits for high-lysine adaptation. Functional assays of 18 representative differentially expressed genes showed that the enhanced expression of multiple candidate genes, especially grpE chaperon, conferred high-lysine stress tolerance in C. glutamicum. Moreover, DNA repair component MutT and energy-transducing NADH dehydrogenase Ndh were also found to be important for protecting cells against high-lysine-induced osmotic stress. Taken together, these aforementioned findings provide broader views of transcriptome profiles and promising candidate targets of C. glutamicum for the adaptation of high-lysine stress during fermentation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum

Wang

Yang

Shi

et al. 2022

Front. Bioeng. Biotechnol.

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“…Cyanobacteria can harbor various exogenous synthesis pathways that achieve similar production rates as Escherichia coli [ 12 ]. Despite the advances in synthetic biology applications, there are still challenges in engineering cyanobacteria for large-scale industrial use [ 13 ]. These include the complexity of the photoautotrophic cell where the distribution of photosynthetic energy and CO 2 are strictly regulated, which can interfere with the bioproduction of specific target chemicals [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Photosynthetically produced sucrose by immobilized Synechocystis sp. PCC 6803 drives biotransformation in E. coli

Tóth

Siitonen

Nikkanen

et al. 2022

Biotechnol Biofuels

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Background Whole-cell biotransformation is a promising emerging technology for the production of chemicals. When using heterotrophic organisms such as E. coli and yeast as biocatalysts, the dependence on organic carbon source impairs the sustainability and economic viability of the process. As a promising alternative, photosynthetic cyanobacteria with low nutrient requirements and versatile metabolism, could offer a sustainable platform for the heterologous production of organic compounds directly from sunlight and CO2. This strategy has been applied for the photoautotrophic production of sucrose by a genetically engineered cyanobacterium, Synechocystis sp. PCC 6803 strain S02. As the key concept in the current work, this can be further used to generate organic carbon compounds for different heterotrophic applications, including for the whole-cell biotransformation by yeast and bacteria. Results Entrapment of Synechocystis S02 cells in Ca2+-cross-linked alginate hydrogel beads improves the specific sucrose productivity by 86% compared to suspension cultures during 7 days of cultivation under salt stress. The process was further prolonged by periodically changing the medium in the vials for up to 17 days of efficient production, giving the final sucrose yield slightly above 3000 mg l−1. We successfully demonstrated that the medium enriched with photosynthetically produced sucrose by immobilized Synechocystis S02 cells supports the biotransformation of cyclohexanone to ε-caprolactone by the E. coli WΔcscR Inv:Parvi strain engineered to (i) utilize low concentrations of sucrose and (ii) perform biotransformation of cyclohexanone to ε-caprolactone. Conclusion We conclude that cell entrapment in Ca2+-alginate beads is an effective method to prolong sucrose production by the engineered cyanobacteria, while allowing efficient separation of the cells from the medium. This advantage opens up novel possibilities to create advanced autotroph–heterotroph coupled cultivation systems for solar-driven production of chemicals via biotransformation, as demonstrated in this work by utilizing the photosynthetically produced sucrose to drive the conversion of cyclohexanone to ε-caprolactone by engineered E. coli.

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“…Microbial cell factories provide sustainable and economically competitive solutions for the manufacturing of chemicals from renewable resources (Ko et al, 2020;Liu et al, 2021;Nielsen et al, 2014). Xylitol is a naturally occurring five-carbon sugar alcohol that is as sweet as sucrose (Salli et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Intelligent self‐control of carbon metabolic flux in SecY‐engineered Escherichia coli for xylitol biosynthesis from xylose‐glucose mixtures

Guo

Ullah

Zheng

et al. 2021

Biotech & Bioengineering

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Xylitol is a salutary sugar substitute that has been widely used in the food, pharmaceutical, and chemical industries. Co-fermentation of xylose and glucose by metabolically engineered cell factories is a promising alternative to chemical hydrogenation of xylose for commercial production of xylitol. Here, we engineered a mutant of SecY proteintranslocation channel (SecY [ΔP]) in xylitol-producing Escherichia coli JM109 (DE3) as a passageway for xylose uptake. It was found that SecY (ΔP) channel could rapidly transport xylose without being interfered by XylB-catalyzed synthesis of xylitol-phosphate, which is impossible for native XylFGH and XylE transporters. More importantly, with the coaction of SecY (ΔP) channel and carbon catabolite repression (CCR), the flux of xylose to the pentose phosphate (PP) pathway and the xylitol synthesis pathway in E. coli could be automatically controlled in response to glucose, thereby ensuring that the mutant cells were able to fully utilize sugars with high xylitol yields. The E. coli cell factory developed in this study has been proven to be applicable to a broad range of xylose-glucose mixtures, which is conducive to simplifying the mixed-sugar fermentation process for efficient and economical production of xylitol.

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Microbial physiological engineering increases the efficiency of microbial cell factories

Cited by 20 publications

References 118 publications

Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum

Transcriptome profiles of high-lysine adaptation reveal insights into osmotic stress response in Corynebacterium glutamicum

Photosynthetically produced sucrose by immobilized Synechocystis sp. PCC 6803 drives biotransformation in E. coli

Intelligent self‐control of carbon metabolic flux in SecY‐engineered Escherichia coli for xylitol biosynthesis from xylose‐glucose mixtures

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