Microbial pathways for advanced biofuel production

Love, John

doi:10.1042/bst20210764

Cited by 12 publications

(5 citation statements)

References 149 publications

(142 reference statements)

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“…The production of fatty alcohols was enhanced in Synechocystis 6803 when a fatty acyl-CoA reductase was expressed, obtained from Simmondsia chinensis. Moreover, when the poly-3-hydroxybutyrate (poly-3HB) and glycogen were prevented from carbon partitioning, an improved production of fatty alcohol was observed [110,111]. When comparing cells treated with the chemical molecule butylated hydroxyanisole (C BHA ) to the control (Co; 163.62 ± 1.57 mgL −1 d −1 ), the biomass and lipid productivities increased by 11% (181.60 ± 1.94 mgL −1 d −1 ) and 48% (18.71 ± 0.20 mgL −1 d −1 ), respectively.…”

Section: Fatty Metabolites (Fatty Alkanes Fatty Alcohols Fatty Acids)mentioning

confidence: 99%

Cyanobacteria as a Biocatalyst for Sustainable Production of Biofuels and Chemicals

Singh,

Jha,

Rana

et al. 2024

Energies

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The combustion of fossil fuels constitutes a significant catalyst for climate change, resulting in the annual release of about two billion tonnes of carbon dioxide (CO2). The increase in CO2 emission is directly linked to a heightened occurrence of natural calamities and health-related issues. The substitution of fossil fuels with renewable energy sources is a fundamental approach to reduce the negative impacts caused by consumption of these nonrenewable energy resources. The utilisation of biological methodologies to produce environmentally friendly energy from renewable sources holds significant potential for the sustainable production of fuel. However, the cultivation of first- and second-generation biofuel crops presents a challenge, since they compete for limited cropland, hence constraining their overall viability. In contrast, photosynthetic microorganisms such as algae and cyanobacteria exhibit significant potential as third-generation biofuel catalysts, devoid of the limitations associated with contemporary biofuels. Cyanobacteria, a type of photosynthetic prokaryotes, exhibit significant potential for the direct conversion of carbon dioxide (CO2) into biofuels, chemicals, and various other valuable compounds. There has been a growing interest in the concept of utilising biological processes to convert carbon dioxide into fuels and chemicals. The introduction of a limited number of heterologous genes has the potential to confer upon cyanobacteria the capability to convert particular central metabolites into a diverse range of end products. The progress in the field of synthetic biology and genetic manipulation has enabled the manipulation of cyanobacteria to synthesise compounds that are not generally produced by these organisms in their natural environment. This study focuses on recent papers that employ various methodologies to engineer cyanobacteria for the purpose of producing high-value compounds, such as biofuels.

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Section: Fatty Metabolites (Fatty Alkanes Fatty Alcohols Fatty Acids)mentioning

confidence: 99%

Cyanobacteria as a Biocatalyst for Sustainable Production of Biofuels and Chemicals

Singh,

Jha,

Rana

et al. 2024

Energies

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“…Microbes can generate biofuels through the efficient conversion of agricultural, domestic and industrial wastes to biogas, ethanol and other higher alcohols and biodiesel (Love, 2022 ; Ramos et al, 2022 ). Biofuels can reduce fossil fuel dependencies, increase energy security and contribute to an overall decrease in greenhouse gas emissions (Field et al, 2020 ; Valdivia et al, 2016 ).…”

Section: How Can Microbial Technologies Contribute To Solutions?mentioning

confidence: 99%

Weaponising microbes for peace

Anand

Hallsworth

Timmis

et al. 2023

Microbial Biotechnology

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Microbes can, of course, be weaponised for war (biowarfare) and terrorism (bioterrorism) through the formulation and delivery of natural or designed pathogens. Less well appreciated, however, and meriting much greater attention, is the exceptional range of goods and services they provide (and are capable of providing) via diverse technologies. Importantly, such technologies can be exploited to reduce or eliminate deficits in basic services, and to promote societal harmony and stability. This preordains microbial technologies as mediators of social equity and peace, the subject treated here.

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“…Efforts are underway to pioneer novel technologies and production platforms for products traditionally produced from gas, coal or oil [2,3]. Many of these advancements use microbial systems to enable conversion of organic materials into biofuels as well as chemicals with a wide range of complexity [4][5][6][7][8]. The replacement of fossil resources with renewable first-generation feedstocks, however, raises social and ethical concerns due to the high demand for alternative food and feed sources driven by the growing world population [9,10].…”

Section: Introductionmentioning

confidence: 99%

Efficient production of itaconic acid from the single carbon substrate methanol with engineeredKomagataella phaffii

Severinsen,

Bachleitner,

Modenese

et al. 2024

Preprint

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Amidst the escalating carbon dioxide levels resulting from fossil fuel consumption, there is a pressing need for sustainable, bio-based alternatives to underpin future global economies. Single carbon feedstocks, derived from CO2, represent promising substrates for biotechnological applications. Especially methanol is gaining prominence for bio-production of commodity chemicals. In this study, we show the potential of Komagataella phaffii as a production platform for itaconic acid using methanol as the carbon source. Successful integration of heterologous genes from Aspergillus terreus (cadA, mttA and mfsA) alongside fine-tuning of the mfsA gene expression, led to promising initial itaconic acid titers of 28 g L-1 after five days of fed-batch cultivation. Through the combined efforts of process optimization and strain engineering strategies we further boosted the itaconic acid production reaching titers of 55 g L-1 after less than five days of methanol feed, whilst increasing the product yield on methanol from 0.06 g g-1 to 0.24 g g-1. Our results highlight the potential of K. phaffii as a methanol-based platform organism for sustainable biochemical production.

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Microbial pathways for advanced biofuel production

Cited by 12 publications

References 149 publications

Cyanobacteria as a Biocatalyst for Sustainable Production of Biofuels and Chemicals

Cyanobacteria as a Biocatalyst for Sustainable Production of Biofuels and Chemicals

Weaponising microbes for peace

Efficient production of itaconic acid from the single carbon substrate methanol with engineeredKomagataella phaffii

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