Microbial Origin of Aquaponic Water Suppressiveness against Pythium aphanidermatum Lettuce Root Rot Disease

Stouvenakers, Gilles; Massart, Sébastien; Depireux, Pierre; Jijakli, M. Haïssam

doi:10.3390/microorganisms8111683

Cited by 17 publications

(43 citation statements)

References 69 publications

(94 reference statements)

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“…Diversity was lower in the rhizospheric water, and diversity and evenness in the stem endosphere, leaf endosphere, and phylloplane in the hydroponic system tended to be more consistent. These results are consistent with the research findings of Stouvenakers (Stouvenakers et al, 2020 ). Microbial diversity and interaction among ecological compartments of plants are related to their ability to resist external disturbance (Kusstatscher et al, 2019 ; Xie et al, 2021 ).…”

Section: Discussionsupporting

confidence: 93%

Colonization and Interaction of Bacteria Associated With Chinese Chives Affected by Ecological Compartments and Growth Conditions

et al. 2022

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Chinese chive has a long history of planting in China. At present, there are many studies on endophytic bacteria and rhizosphere microorganisms of Chinese chive, but the effects of ecological compartment and growth conditions on bacterial communities in Chinese chives are unclear. Here, we aimed to elucidate the differences in bacterial a-diversity, β-diversity, community structure, core species differences, interaction networks and predicted metabolic functions among bacterial communities in different ecological compartments (the phylloplane, leaf endosphere, stem endosphere, root endosphere, and rhizosphere) in Chinese chives in an open field, a solar greenhouse, an arched shed, and a hydroponic system. Sixty samples were collected from these five ecological compartments under four growth conditions, and we compared the bacterial profiles of these groups using 16S rRNA sequencing. We evaluated the differences in diversity and composition among bacterial communities in these ecological compartments, analyzed the bacterial interaction patterns under the different growth conditions, and predicted the bacterial metabolic pathways in these ecological compartments and growth conditions. The results showed that the effects of ecological compartments on bacterial diversity, community composition, interaction network pattern, and functional expression of Chinese chives were greater than those of growth condition. Ecological compartments (R2 = 0.5292) could better explain bacterial community division than growth conditions (R2 = 0.1056). The microbial interaction networks and indicator bacteria in different ecological compartments showed that most of the bacteria that played the role of key nodes (OTUs) in each ecological compartment were bacteria with high relative abundance in the compartment. However, the bacteria that played the role of key nodes (OTUs) in bulbs were not Proteobacteria with the highest relative abundance in the compartment, but Actinobacteria that were significantly enriched in the root endosphere and rhizosphere ecological compartments. In addition, interactions among bacteria were interrupted in the hydroponic system, and specific bacterial communities and interaction patterns in Chinese chives varied among growth conditions. Prediction of metabolic functions indicated that plant metabolic activity related to stress responses and induction of system resistance was greater in belowground ecological compartments.

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Section: Discussionsupporting

confidence: 93%

Colonization and Interaction of Bacteria Associated With Chinese Chives Affected by Ecological Compartments and Growth Conditions

et al. 2022

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“…At the genus level, the composition of both compartments is also coherent with previous results obtained from the same aquaponic system (Eck et al, 2019(Eck et al, , 2020Stouvenakers et al, 2020). Indeed, the predominant families/genera are the Burkholderiaceae family, the Flavobacterium genus and the Hydrogenophaga genus, albeit in different proportions in both compartments.…”

Section: Composition Of the Samplessupporting

confidence: 89%

“…Meanwhile, Sanchez et al (2019) tackled a different angle of the microorganisms study in aquaponics by focusing on the potentially plant beneficial functions harbored by the bacterial communities in aquaponics. On the other side, Stouvenakers et al (2020) investigated the suppressiveness activity of aquaponic water on specific plant pathogens such as Pythium aphanidermatum. Furthermore, a E-mail: mh.jijakli@uliege.be it is now known that the plants present in the system can greatly influence the composition of the microorganisms communities as shown by Eck et al (2019) when comparing the same system with and without the hydroponic compartment and as confirmed by Bartelme et al (2019).…”

Section: Introductionmentioning

confidence: 99%

Microorganisms in aquaponics: insights on the composition of the root microbiome of lettuces of varying age

Eck¹,

Szekely²,

Massart³

et al. 2021

Acta Hortic.

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Aquaponics is a developing, soilless production technique combining hydroponics and recirculating aquaculture and is now spreading worldwide. Nevertheless, several aspects of aquaponics still need research. Indeed, despite being key-players in the dynamic equilibrium of aquaponic systems, microorganisms and their roles in aquaponics are still scarcely known. The aim of this study is thus to explore the microorganisms communities thriving in the root compartments of lettuce in the closed-loop aquaponic system of Gembloux Agro-Bio Tech and to focus on the differences between the microbial communities of lettuce of varying age. Therefore, root samples were collected from lettuces of five different age groups and microorganisms from the rhizoplane and from the endosphere were harvested. DNA was then extracted and sequenced on an Illumina MiSeq platform, targeting the V1-V3 region of the 16S rRNA gene. Results show that no significant difference could be noted between the different age groups despite a visible trend on the Bray-Curtis PCoA. However, significant differences in alpha-and beta-diversity could be observed between the rhizoplane and endosphere compartments. In terms of taxonomy, the composition of the root community is similar to what can be found in the literature and coherent with the previous experiments conducted in the same aquaponic system.

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“…Despite these benefits, the simultaneous presence of fish, plant, and beneficial bacteria in the same water loop (especially in coupled aquaponics), has made pest and disease management a pressing challenge in aquaponics, limiting its commercialization (Stouvenakers et al, 2020). Therefore, there is a need to establish control methods with little or no negative influence on plants, fish, and beneficial bacteria.…”

Section: Introductionmentioning

confidence: 99%

Potential use of entomopathogenic and mycoparasitic fungi against powdery mildew in aquaponics

Folorunso

Bohatá

Kavkova³

et al. 2022

Front. Mar. Sci.

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Aquaponics has the potential to produce sustainable and accessible quality food through the integration of hydroponics and aquaculture. Plants take up dissolved nutrients in fish wastewater, allowing water reuse for fish. However, the simultaneous presence of fish and plants in the same water loop has made phytosanitary treatments of diseases such as powdery mildew problematic due to risks of toxicity for fish and beneficial bacteria, limiting its commercialization. Entomopathogenic and mycoparasitic fungi have been identified as safe biological control agents for a broad range of pests. This study aimed to investigate the efficacy of entomopathogenic fungi, Lecanicillium attenuatum (LLA), Isaria fumosorosea (IFR), and mycoparasitic fungus Trichoderma virens (TVI) against Podosphaera xanthii. Also, we investigated the possible harmful effects of the three fungal biocontrol agents in aquaponics by inoculating them in aquaponics water and monitoring their survival and growth. The findings showed that the three biocontrol agents significantly suppressed the powdery mildew at 107 CFU/ml concentration. Under greenhouse conditions (65-73% relative humidity (RH)), a significant disease reduction percentage of 85% was recorded in L. attenuatum-pretreated leaves. IFR-treated leaves had the least AUDPC (area under disease progress curve) of ~434.2 and disease severity of 32% under 65-73% RH. In addition, L. attenuatum spores were the most persistent on the leaves, the spores population increased to 9.54 × 103 CFUmm-2 from the initial 7.3 CFUmm-2 under 65-73%. In contrast, in hydroponics water, the LLA, IFR, and TVI spores significantly reduced by more than 99% after 96 hrs. Initial spore concentrations of LLA of 107 CFU/ml spores were reduced to 4 x 103 CFU after 96 hrs. Though the results from this study were intended for aquaponics systems, relevance of the results to other cultivation systems are discussed.

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Microbial Origin of Aquaponic Water Suppressiveness against Pythium aphanidermatum Lettuce Root Rot Disease

Cited by 17 publications

References 69 publications

Colonization and Interaction of Bacteria Associated With Chinese Chives Affected by Ecological Compartments and Growth Conditions

Colonization and Interaction of Bacteria Associated With Chinese Chives Affected by Ecological Compartments and Growth Conditions

Microorganisms in aquaponics: insights on the composition of the root microbiome of lettuces of varying age

Potential use of entomopathogenic and mycoparasitic fungi against powdery mildew in aquaponics

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