Microbial O2- and H2O2-electrode sensors for alcohol assays based on the use of permeabilized mutant yeast cells as the sensitive bioelements

Гончар, М. В.; Maidan, Mykola; Moroz, O. M.; Woodward, John R.; Sibirny, Andriy А.

doi:10.1016/s0956-5663(98)00034-7

Cited by 59 publications

(33 citation statements)

References 16 publications

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“…The whole cells based biosensors were reported to be simpler and less expensive than the purified enzyme biosensors due to the purification costs of enzymes. 11,49,[64][65][66][67] Furthermore, whole cell tissue materials from plants and animals could be incorporated in carbon paste electrodes which was a more direct approach than the whole cell materials with a Clark oxygen electrode. 49,65,66,68 Even though the experimental techniques were similar to the direct electrochemical techniques of Wang and Lin, 66 a plant tissue (apple, banana, and potato) paste electrode of this report could be used in differential pulse voltammetry, rotating disc electrode voltammetry, and amperometry with flow injection analysis.…”

Section: ·2 Tissue Biosensorsmentioning

confidence: 99%

“…58 Microbial/electrochemical oxygen and peroxide electrodes for alcohol determination were proposed to enhance the alcohol activity and to impair the catalase activity by using appropriate mutants of methylotrophic yeasts Hansenula polymorpha, the sensitive bioelements. 67 The selected mutations used for the oxygen electrodes allowed an increase in the respiration response of mutant cells towards alcohols, whereas in the case of the cells used with a peroxide biosensor, the mutant cells elevated the excretion of hydrogen peroxide during metabolic transformation of alcohol. Thus, the effects of chemical permeabilization on the respiration and hydrogen peroxide production enhanced sensitivity and selectivity of the biosensors by the sensing cells' generic manipulation without isolation or purification of the enzymes.…”

Section: ·2 Tissue Biosensorsmentioning

confidence: 99%

“…Both types of the biosensors yielded lower sensitivity of 0.2 -0.4 mM for ethanol and 0.03 -0.05 mM for methanol; no sample pretreatment and no additional cofactors were used in the evaluation of these biosensors. 67 Even though oxygen and peroxide electrodes were selective, sensitive, and reliable for methanol and ethanol, no signal was observed with glucose and glycerol as substrates. The enhanced sensitivity and selectivity of the response of the sensing cells were directly related to their genetic manipulation without any isolation or purification of the enzymes.…”

Section: ·2 Tissue Biosensorsmentioning

confidence: 99%

“…The overall performance of these biosensors had better results than were obtained in the studies of Katrlik and co-workers and Eggins and co-workers. [64][65][66][67] …”

Section: ·2 Tissue Biosensorsmentioning

confidence: 99%

“…49,[64][65][66][67]72,73 Such analytical tools exhibited lower sensitivity than the biosensors based on the same enzyme in aqueous media, because of the organic media ability to reduce the rate of enzymatic reaction. 73 Lactitol, an uncharged polyhydroxyl, was reported to constitute an efficient additive for the improvement of the analytical characteristics of amperometric biosensors since it had highly hydrophilic properties and the capacity to replace bound water via hydrogen bonding with the enzyme.…”

Section: ·6 Biological Active Electrodesmentioning

confidence: 99%

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Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

2004

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The objective of this study is to analyze the technical importance, performance, techniques, advantages, and disadvantages of the biosensors in general and of the electrochemical biosensors in particular. A product of reaction diffuses to the transducer in the first generation biosensors (based on Clark biosensors). The mediated biosensors or second generation biosensors use specific mediators between the reaction and the transducer to improve sensitivity. The second generation biosensors involve two steps: first, there is a redox reaction between enzyme and substrate that is reoxidized by the mediator, and eventually the mediator is oxidized by the electrode. No normal product or mediator diffusion is directly involved in the third generation biosensors, direct biosensors. Based on the type of transducer, current biosensors are divided into optical, mass, thermal, and electrochemical sensors. They are used in medical diagnostics, food quality controls, environmental monitoring, and other applications. These biosensors are also grouped under two broad categories of sensors: direct and indirect detection systems. Moreover, these systems could be further grouped into continuous or batch operation. Therefore, amperometric biosensors and their current applications are focused on more in detail since they are the most commonly used biosensors in monitoring and diagnosing tests in clinical analysis. Problems related to the commercialization of medical, environmental, and industrial biosensors as well as their performance characteristics, their competitiveness in comparison to the conventional analytical tools, and their costs determine the future development of these biosensors.

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Section: ·2 Tissue Biosensorsmentioning

confidence: 99%

Section: ·2 Tissue Biosensorsmentioning

confidence: 99%

Section: ·2 Tissue Biosensorsmentioning

confidence: 99%

“…The overall performance of these biosensors had better results than were obtained in the studies of Katrlik and co-workers and Eggins and co-workers. [64][65][66][67] …”

Section: ·2 Tissue Biosensorsmentioning

confidence: 99%

Section: ·6 Biological Active Electrodesmentioning

confidence: 99%

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Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

2004

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A Reusable Interface Constructed by 3‐Aminophenylboronic Acid‐Functionalized Multiwalled Carbon Nanotubes for Cell Capture, Release, and Cytosensing

Zhong¹,

Bai²,

Xu³

et al. 2010

Adv Funct Materials

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A newly developed electrochemical cell sensor for the determination of K562 leukemia cells using 3‐aminophenylboronic acid (APBA)‐functionalized multiwalled carbon nanotubes (MWCNTs) films is demonstrated. The films are generated by the covalent coupling between the NH2 groups in APBA and the COOH group in the acid‐oxidized MWCNTs. As a result of the sugar‐specific affinity interactions, the K562 leukemia cells are firmly bound to the APBA‐functionalized MWCNTs film via boronic acid groups. Compared to electropolymerized APBA films, the presence of MWCNTs not only provides abundant boronic acid domains for cell capture, their high electrical conductivity also makes the film suitable for electrochemical sensing applications. The resulting modified electrodes are tested as cell detection sensors. This work presents a promising platform for effective cell capture and constructing reusable cytosensors.

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Glucose‐induced production of recombinant proteins in Hansenulapolymorpha mutants deficient in catabolite repression

Krasovska

Stasyk

Nahorny

et al. 2007

Biotech & Bioengineering

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The most commonly used expression platform for production of recombinant proteins in the methylotrophic yeast Hansenula polymorpha relies on the strong and strictly regulated promoter from the gene encoding peroxisomal enzyme alcohol (or methanol) oxidase (P(MOX)). Expression from P(MOX) is induced by methanol and is partially derepressed in glycerol or xylose medium, whereas in the presence of hexoses, disaccharides or ethanol, it is repressed. The need for methanol for maximal induction of gene expression in large-scale fermentation is a significant drawback, as this compound is toxic, flammable, supports a slow growth rate and requires extensive aeration. We isolated H. polymorpha mutants deficient in glucose repression of P(MOX) due to an impaired HpGCR1 gene, and other yet unidentified secondary mutations. The mutants exhibited pronounced defects in P(MOX) regulation only by hexoses and xylose, but not by disaccharides or ethanol. With one of these mutant strains as hosts, we developed a modified two-carbon source mode expression platform that utilizes convenient sugar substrates for growth (sucrose) and induction of recombinant protein expression (glucose or xylose). We demonstrate efficient regulatable by sugar carbon sources expression of three recombinant proteins: a secreted glucose oxidase from the fungus Aspergillus niger, a secreted mini pro-insulin, and an intracellular hepatitis B virus surface antigen in these mutant hosts. The modified expression platform preserves the favorable regulatable nature of P(MOX) without methanol, making a convenient alternative to the traditional system.

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Microbial O2- and H2O2-electrode sensors for alcohol assays based on the use of permeabilized mutant yeast cells as the sensitive bioelements

Cited by 59 publications

References 16 publications

Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

Recent Developments, Characteristics, and Potential Applications of Electrochemical Biosensors

A Reusable Interface Constructed by 3‐Aminophenylboronic Acid‐Functionalized Multiwalled Carbon Nanotubes for Cell Capture, Release, and Cytosensing

Glucose‐induced production of recombinant proteins in Hansenulapolymorpha mutants deficient in catabolite repression

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