Microbial nanoenzymes: Features and applications

Sami, Adnan; Haider, Muhammad Zeeshan; Shafiq, Muhammad

doi:10.1016/b978-0-323-95241-5.00015-0

Cited by 7 publications

(3 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Clade I include 41 NPR-related proteins, and clade II comprised 16 NPR-related proteins. Proteins from the same clade appear to be structurally and functionally related ( Sami et al., 2024 ).…”

Section: Resultsmentioning

confidence: 99%

“…The full-length sequences of Arabidopsis proteins and the protein sequences of NPR1-like genes in 10 different citrus species were aligned using the Clustal W method (Nakasu et al, 2019). As a result, an unrooted phylogenetic tree was generated using the maximum-likelihood (ML) algorithm, employing MEGA-X software, with the specified parameters including the bootstrap method (1,000 replicates), the Jones-Taylor-Thornton model, and the inclusion of all sites (Shafiq et al, 2024).…”

Section: Phylogeny Analysismentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide analysis of NPR1-like genes in citrus species and expression analysis in response to citrus canker (Xanthomonas axonopodis pv. citri)

Ali,

Shafiq,

Haider

et al. 2024

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Citrus fruits, revered for their nutritional value, face significant threats from diseases like citrus canker, particularly impacting global citrus cultivation, notably in Pakistan. This study delves into the critical role of NPR1-like genes, the true receptors for salicylic acid (SA), in the defense mechanisms of citrus against Xanthomonas axonopodis pv. citri (Xcc). By conducting a comprehensive genome-wide analysis and phylogenetic study, the evolutionary dynamics of Citrus limon genes across diverse citrus cultivars are elucidated. Structural predictions unveil conserved domains, such as the BTB domain and ankyrin repeat domains, crucial for the defense mechanism. Motif analysis reveals essential conserved patterns, while cis-regulatory elements indicate their involvement in transcription, growth, response to phytohormones, and stress. The predominantly cytoplasmic and nuclear localization of NPR1-like genes underscores their pivotal role in conferring resistance to various citrus species. Analysis of the Ks/Ka ratio indicates a purifying selection of NPR1-like genes, emphasizing their importance in different species. Synteny and chromosomal mapping provide insights into duplication events and orthologous links among citrus species. Notably, Xac infection stimulates the expression of NPR1-like genes, revealing their responsiveness to pathogenic challenges. Interestingly, qRT-PCR profiling post-Xac infection reveals cultivar-specific alterations in expression within susceptible and resistant citrus varieties. Beyond genetic factors, physiological parameters like peroxidase, total soluble protein, and secondary metabolites respond to SA-dependent PR genes, influencing plant characteristics. Examining the impact of defense genes (NPR1) and plant characteristics on disease resistance in citrus, this study marks the inaugural investigation into the correlation between NPR1-associated genes and various plant traits in both susceptible and resistant citrus varieties to citrus bacterial canker.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Phylogeny Analysismentioning

confidence: 99%

Genome-wide analysis of NPR1-like genes in citrus species and expression analysis in response to citrus canker (Xanthomonas axonopodis pv. citri)

Ali,

Shafiq,

Haider

et al. 2024

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is not just a food item but a cultural and economic anchor (Birla et al, 2017). Through the course of generations, Oryza sativa and Oryza glaberrima, the two main cultivated rice species, have been subjected to selective breeding, which resulted in the development of the wide array of varieties with different traits (Sami et al, 2024). If the Basmati rice of the Indian subcontinent is aromatic, the sushi rice from Japan is short-grained, and sticky rice is essential in Southeast Asian cuisines, then rice is a canvas used by culinary traditions to paint their flavor profiles (Ito & Lacerda, 2019).…”

Section: Introductionmentioning

confidence: 99%

The Contribution of Transgenic Rice to Enhance Grain Yield

JAVED,

SAMI,

HAIDER

et al. 2024

Bull. Biol. All. Sci. Res.

View full text Add to dashboard Cite

Breeders' main goal is to increase the proportion of high-quality rice produced overall. To create effective rice breeding strategies, possible yield-related loci have been mined. many researchers are using transgenic strategies as cutting-edge methods to increase rice productivity. Quantitative trait loci (QTLs) play a pivotal role in governing grain yield in Oryza sativa L., commonly known as rice. The genes contributing to QTLs that determine grain size, length, and weight have been successfully identified. Numerous genes are upregulated to enhance the overall yield of rice. Recent advancements have led to the discovery of genes and QTLs specifically associated with rice yield. Through an in-depth analysis of various yield characteristics, including grain weight, thousand grain weight, grain length, grain width, grain yield per plant, grain number per panicle, and panicles per plant, we conducted a comprehensive review using extensive literature research and public domain databases. Additionally, we explored the progress made in transgenic technology and advanced genomic techniques. The compiled information on genes and QTLs related to yield enhancement aims to provide a valuable resource. The integrated analysis of existing data on genes and/or QTLs provide evidence on potential combinations for creating superior genotypes that combine high yield across multiple traits. Integration of molecular markers, transgenic techniques and conventional breeding as discussed in this extensive review opens up the prospect of developing high yielding rice varieties

show abstract

Genome-wide identification and in-silico expression analysis of CCO gene family in sunflower (Helianthus annnus) against abiotic stress

Sami,

Haider,

Shafiq

et al. 2024

Plant Mol Biol

Self Cite

View full text Add to dashboard Cite

Carotenoid cleavage oxygenases (CCOs) enzymes play an important role in plant growth and development by producing a wide array of apocarotenoids and their derivatives. These compounds are vital for colouring flowers and fruits and synthesizing plant hormones such as abscisic acid and strigolactones. Despite their importance, the gene family responsible for CCO enzymes in sunflowers has not been identified. In this study, we identify the CCO genes of the sunflower plant to fill this knowledge gap. Phylogenetic and synteny analysis indicated that the Helianthus annnus CCO (HaCCO) genes were conserved in different plant species and they could be divided into three subgroups based on their conserved domains. Analysis using MEME tool and multiple sequence alignment identified conserved motifs in the HaCCO gene sequence. Cis-regulatory elements (CREs) analysis of the HaCCO genes indicated the presence of various responsive elements related to plant hormones, development, and responses to both biotic and abiotic stresses. This implies that these genes may respond to plant hormones, developmental cues, and drought stress, offering potential applications in the development of more resistant crops. Genes belonging to the 9-cis-epoxy carotenoid dioxygenases (NCED) subgroups predominantly exhibited chloroplast localization, whereas the genes found in other groups are primarily localized in the cytoplasm. These 21 identified HaCCOs were regulated by 60 miRNAs, indicating the crucial role of microRNAs in gene regulation in sunflowers. Gene expression analysis under drought stress revealed significant up-regulation of HaNCED16 and HaNCED19, genes that are pivotal in ABA hormone biosynthesis. During organ-specific gene expression analysis, HaCCD12 and HaCCD20 genes exhibit higher activity in leaves, indicating a potential role in leaf pigmentation. This study provides a foundation for future research on the regulation and functions of the CCO gene family in sunflower and beyond. There is potential for developing molecular markers that could be employed in breeding programs to create new sunflower lines resistant to biotic and abiotic stresses.

show abstract

Microbial nanoenzymes: Features and applications

Cited by 7 publications

References 69 publications

Genome-wide analysis of NPR1-like genes in citrus species and expression analysis in response to citrus canker (Xanthomonas axonopodis pv. citri)

Genome-wide analysis of NPR1-like genes in citrus species and expression analysis in response to citrus canker (Xanthomonas axonopodis pv. citri)

The Contribution of Transgenic Rice to Enhance Grain Yield

Genome-wide identification and in-silico expression analysis of CCO gene family in sunflower (Helianthus annnus) against abiotic stress

Contact Info

Product

Resources

About