Microbial models of mammalian metabolism: biotransformations of phenacetin and its<i>O</i>-alkyl homologues with<i>Cunninghamella</i>species

Reddy, Claire; Acosta, Daniel; Davis, Patrick J.

doi:10.3109/00498259009046627

Cited by 8 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PolyP accumulation has been demonstrated for a wide variety of microorganisms, although the conditions favouring this accumulation differ markedly (29,30,35,36,44,58,74). In general polyP concentration is low during the exponential growth but may increase either, when the stationary phase begins or when growth is arrested due to nutrient imbalance (24,42,58,71).…”

Section: Discussionmentioning

confidence: 99%

Effects of phosphorus on polyphosphate accumulation by Cunninghamella elegans

Lima

Nascimento

Souza

et al. 2003

Braz. J. Microbiol.

View full text Add to dashboard Cite

The content of inorganic polyphosphate and the polymeric degree of these compounds were evaluated during the growth of Cunninghamella elegans in medium containing varying orthophosphate (Pi) concentrations. For this purpose, a combination of chemical methods for polyphosphate extraction and ultrastructural cytochemistry were used. The orthophosphate and glucose consumption was also determined during the fungal cultivation. At Pi concentrations of 0.5, 2.5 and 0.0 g/L, the maximum amounts of biomass were 3.18, 3.29 and 0.24 g/L, respectively. During growth the cells accumulated Pi from the medium. At three days of growth the biomass consumed up to 100 and 95% of Pi from the media at initial concentrations of 0.5 and 2.5 g/L, respectively. Polyphosphate was observed at different Pi concentrations in medium and at different stages of growth. Polyphosphate was assayed by the content of labile phosphorus in water, acid-insoluble and alkali-soluble fractions. The content of fractions changed according to phosphorus concentration in the media and growth phase. During growth on all three media used, the cytochecmical behavior of polyphosphate changed considerably. The results obtained in this study reveal a potential of Cunninghamella elegans in the polyphosphate accumulation, and suggest a future application in the biotechnological processes.

show abstract