Microbial Metalloproteomics

Abstract

Metalloproteomics is a rapidly developing field of science that involves the comprehensive analysis of all metal-containing or metal-binding proteins in a biological sample. The purpose of this review is to offer a comprehensive overview of the research involving approaches that can be categorized as inductively coupled plasma (ICP)-MS based methods, X-ray absorption/fluorescence, radionuclide based methods and bioinformatics. Important discoveries in microbial proteomics will be reviewed, as well as the outlook to new emerging approaches and research areas.

Structural and biochemical analysis of a phosin fromStreptomyces chartreusisreveals a combined polyphosphate‐ and metal‐binding fold

“…The presence of metal ions was initially deduced from unusually high‐local electron densities (16 σ and 6.8 σ respectively) as well as the characteristic coordination of His side chains. Since it was not possible to establish the identity of the bound ions on the basis of our crystallographic data and the observed coordination chemistry alone , we analysed the metal content of PptA crystals by X‐ray fluorescence (XRF) . This experiment unambiguously revealed the presence of Cu 2+ .…”

“…X‐ray fluorescence analysis of PptA crystals was performed at beamline BL14.1 operated by the Joint Berlin MX‐Laboratory at the BESSY II electron storage ring (Berlin‐Adlershof) . Metal concentrations were determined under strongly denaturing conditions (in the presence of 8 m urea) according to the Zincon method described by Säbel et al .…”

Advances in element speciation analysis of biomedical samples using synchrotron-based techniques

“…For works published in previous periods the reader may refer to several valuable review articles [5][6][7][8][9]. This review will be limited to the application of XAS on biomedical samples, but interesting examples of XAS element speciation of bioenvironmental samples such as plants or micro-organisms can be found in other recent articles [10][11][12][13]. Due to the simultaneous measurement of many important parameters, XAS is among the best techniques for determining the "structure-to-function" relationship in metalloprotein studies.…”

Comparação do perfil proteômico através da técnica de espectrometria de massas dos fungos Aspergillus niger E Rhizopus microsporus submetidos à estresse pela adição de cobre.

“…FENG et al,2017). Os fungos possuem mecanismos de absorção de metais além de um amplo sistema regulatório e biossintético para a incorporação dos íons metálicos a proteínas(HAGEDOORN, 2015). O excesso de cobre leva a distúrbios metabólicos, podendo ocasionar graves danos ao crescimento e desenvolvimento das células, influenciando no proteôma fúngico(BANCI et al, 2011).…”

“…Uma das principais funções da mitocôndria é a geração de ATP através dos complexos respiratórios que transportam os elétrons obtidos a partir da oxidação de NADH e FADH até o citocromo para a redução do oxigênio(RICHTER et al, 2010).Proteínas como cytochrome P450, malic enzyme, acyl-CoA desaturase, oxidoreductase activity atuando em presença de NAD(P)H foram codificadas em presença do agente oxidante. Responsáveis por reações de oxido-redução (redox), estas proteínas se ligam ao substrato atuando na tranferência de elétrons, em que o oxigênio molecular é reduzido ou incorporado como um doador de elétrons, ocorrendo interação seletiva e não covalente com os íons presentes no meio, alterando assim o metabolismo da célula(HAGEDOORN, 2015). A expressão de proteínas do citosol pelo complexo TOM, não foram prejudicadas, como é sugerido pela presença das subunidades cytochrome b5 e cytochrome P450 expressas em presença de cobre.…”