Microbial metal-binding mechanisms and their relation to nuclear waste disposal

McLean, Robert; Fortin, Danielle; Brown, D. Ann

doi:10.1139/m96-055

Cited by 87 publications

(51 citation statements)

References 70 publications

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“…The peripheral deposition of Ni within the cells conformed to reports on P. aeruginosa strains, which showed the sequestration of intracellular Ni or U in and around the cell periphery (Choudhary and Sar 2010;Sar et al 2001). Such localized metal cation deposition in the cell envelope is attributed to the phosphoryl and carboxyl moieties in the cell wall as well as to membrane components (McLean et al 1996).…”

Section: Discussionsupporting

confidence: 86%

Aerobic bioreduction of nickel(II) to elemental nickel with concomitant biomineralization

Zhan

Zhang

2012

Appl Microbiol Biotechnol

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Although microorganisms have the potential to reduce metals, products with elementary forms are unusual. In the present study, a strain of Pseudomonas sp. MBR was tested for its ability to reduce metal ions to their elementary forms coupled to biomineralization under aerobic conditions. The Pseudomonas sp. MBR strain was able to reduce metals such as Fe(III), Mn(II), Cu(II), Ni(II), Cd(II), Co(II), Al(III), Se(IV), and Te(IV) as electron acceptors to elementary forms using citrate, lactate, pyruvate, succinate, malate, glucose, or ethanol as electron donors. Growth and reduction during biomineralization occurred within the pH range of 6.0 to 11.0 and temperature range of 4 to 40°C, with an optimum growth temperature of 28°C. The resistance of Ni (II) varied from 0.5 to 5 mM. Ni(II) reduction was still observed when nitrate was present in addition to oxygen as a potential electron acceptor. The Ni(II) reduction efficiency was related with the molar ratio of the electron donor to Ni(II). Unlike other dissimilatory metal-reducing bacteria, which oxidizes organic matter with Fe(III) or Mn (IV) as the sole electron acceptor coupled to energy production under facultative anaerobic conditions, this strain used oxygen as an electron acceptor combined with metal reduction. The aerobic metal reduction may relate to a co-metabolic reduction. Transmission electron microscopy images demonstrated that the cells had the ability to accumulate heavy metals, and that the detoxicity mechanism was intracellular metal reduction. These results suggested that the use of Pseudomonas sp. MBR could be promising for toxic heavy metal bioremediation and biological metallurgy.

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Section: Discussionsupporting

confidence: 86%

Aerobic bioreduction of nickel(II) to elemental nickel with concomitant biomineralization

Zhan

Zhang

2012

Appl Microbiol Biotechnol

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“…However, knowing that the EPS of microorganisms readily bind a variety of metals (McLean et al 1996) and that Fe(II1)-oxyhydroxide coatings readily a n d irreversibly bind bacterial cells (Mills e t al. 1994), 3 complementary processes could at least partly explain the accumulation of ferric iron minerals on the bivalve: (1) the deposition of ferric iron within bacterial EPS in the outer layer, (2) the accumulation of heavily ferric iron-encrusted bacteria would form the intermediary layer, (3) the degradation of the bacteria a n d EPS would lead to the subsequent accumulation of the ferric iron minerals, forming the inner layer.…”

Section: Discussionmentioning

confidence: 99%

Morphology of a ferric iron-encrusted biofilm forming on the shell of a burrowing bivalve (Mollusca)

Gillan¹,

Ridder²

1997

Aquat. Microb. Ecol.

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The shell of the bivalve Montacuta ferruginosa is usually covered with a rust-coloured and ferric iron-encrusted biofilm. The latter is a structured microbial mat with 3 separate layers. The outer layer is essentially microbial (f~lamentous bacteria and protozoa). The intermediary layer is both microblal and mineral (heavily fernc iron-encrusted filamentous bacteria and protozoa) The inner layer is essentially mineral (ferric iron deposits) and generally devoid of living microorgan~sms. The most abundant microorganisms in the biofilm are filamentous bacteria related to Beggiatoaceae. The genesis of this mineral-microbial mat could be partly due to a sequence of processes: (1) ferric iron deposition within bacterial sheaths in the outer layer; (2) the release and accumulation of heavily ferric ironencrusted sheaths after lysis of the bacteria in the intermediary layer; (3) degradation of bacterial sheaths and accumulation of ferric iron minerals in the inner layer.

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“…Biofilms have been implicated in a number of problems, including many infections, industrial fouling, and corrosion (Costerton et al, 1987;McLean et al, 1996). Several detailed studies have been conducted on biofilms to ascertain the differences between planktonic and biofilm bacteria.…”

Section: Introductionmentioning

confidence: 99%

A previously uncharacterized gene, yjfO (bsmA), influences Escherichia coli biofilm formation and stress response

et al. 2010

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Bacteria growing as surface-adherent biofilms are better able to withstand chemical and physical stresses than their unattached, planktonic counterparts. Using transcriptional profiling and quantitative PCR, we observed a previously uncharacterized gene, yjfO to be upregulated during Escherichia coli MG1655 biofilm growth in a chemostat on serine-limited defined medium. A yjfO mutant, developed through targeted-insertion mutagenesis, and a yjfO-complemented strain, were obtained for further characterization. While bacterial surface colonization levels (c.f.u. cm "2 ) were similar in all three strains, the mutant strain exhibited reduced microcolony formation when observed in flow cells, and greatly enhanced flagellar motility on soft (0.3 %) agar. Complementation of yjfO restored microcolony formation and flagellar motility to wild-type levels. Cell surface hydrophobicity and twitching motility were unaffected by the presence or absence of yjfO. In contrast to the parent strain, biofilms from the mutant strain were less able to resist acid and peroxide stresses. yjfO had no significant effect on E. coli biofilm susceptibility to alkali or heat stress. Planktonic cultures from all three strains showed similar responses to these stresses. Regardless of the presence of yjfO, planktonic E. coli withstood alkali stress better than biofilm populations. Complementation of yjfO restored viability following exposure to peroxide stress, but did not restore acid resistance. Based on its influence on biofilm maturation and stress response, and effects on motility, we propose renaming the uncharacterized gene, yjfO, as bsmA (biofilm stress and motility).

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Microbial metal-binding mechanisms and their relation to nuclear waste disposal

Cited by 87 publications

References 70 publications

Aerobic bioreduction of nickel(II) to elemental nickel with concomitant biomineralization

Aerobic bioreduction of nickel(II) to elemental nickel with concomitant biomineralization

Morphology of a ferric iron-encrusted biofilm forming on the shell of a burrowing bivalve (Mollusca)

A previously uncharacterized gene, yjfO (bsmA), influences Escherichia coli biofilm formation and stress response

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