Microbial Metabolism of Quinoline and Related Compounds. II. Degradation of Quinoline byPseudomonas fluorescens3,Pseudomonas putida86 andRhodococcus spec.B1

Cloning, Expression, and Sequence Analysis of the Three Genes Encoding Quinoline 2-Oxidoreductase, a Molybdenum-containing Hydroxylase from 86

“…However, 2-oxo-1,2-dihydroquinoline accumulated only transiently and was consumed further. Since P. putida mt-2 KT2440 does not utilize 2-oxo-1,2-dihydroquinoline, this result indicates that not only the qor genes, but also genes encoding further enzymes of the "coumarin pathway" of quinoline degradation (Schwarz et al, 1989;Shukla, 1989) are located on the recombinant cosmid of clone 13/42. The specific activity of quinoline 2-oxidoreductase in cell-free extracts of clone 13/42 and of wildtype P. putida 86 was 0.37 unit/mg protein and 0.72 unit/mg protein, respectively.…”

“…Degradation proceeds via the "coumarin pathway" (Schwarz et al, 1989;Shukla, 1989). In the first step, quinoline 2-oxidoreductase (Qor) 1 catalyzes the hydroxylation to 2-oxo-1,2-dihydroquinoline with concomitant reduction of a suitable electron acceptor.…”

Microorganisms in Chemistry of Nitrogenous Heterocycles

“…Культура Rhodococcus sp. Превра-щала хинолин (CLXXXIV) не только в соединения (CLXXXV) и (CLXXXVI), но и в 5-гидрокси-6-(3-карбокси-3-оксопропенил)-1H-2-пиридон (CLXXXVII), а также 2H-пирано-2-он-[3,2b]-5H-6-пиридон (CLXXXVIII) [Schwarz et al, 1989]: [Shukla, 1986;Shukla, 1987;Schwarz et al, 1989;Aislabie et al, 1990;Kilbane et al, 2000]: дигидрохинолин (CXCVII) и антраниловую кислоту (CXCVIII) [Boyd et al, 1987;Boyd et al, 1993]: [Boyd et al, 2002]. В тоже время культура Desulfobacterium indolicum в анаэробных условиях окисляла хинолин (CLXXXIV) до 2-оксо-1,2-дигидрохинолина (CLXXXV), а затем до 2-оксо-1,2,3,4-тетрагидрохинолина (CXCIX) .Оксидаза, выделенная из Arthrobacter sp.…”

Bacterial Degradation of Quinoline and Derivatives—Pathways and Their Biocatalysts

“…[20] Two strains belonging to the Gramnegative pseudomonads and the Gram-positive Rhodococcus strain B1 were chosen for more detailed investigation. From metabolic intermediates found in the growth medium, two different pathways for the degradation of quinoline were proposed [21] (Sections 2.3 and 2.5). In the initial step of both pathways, quinoline is converted into 1H-2-oxoquinoline.…”

“…[24] c) 7,8-Dihydroxy-1H-2-oxoquinoline pathway: degradation of 4-methylquinoline by Pseudomonas putida K1. [26] d) 8-Hydroxycoumarin pathway: degradation of quinoline (10) by Pseudomonas putida 86, [21] Pseudomonas fluorescens 3, [21] Pseudomonas stutzeri, and Pseudomonas pseudoalcaligenes. [32] Scheme 2.…”