Microbial lipid production by Rhodosporidium toruloides under sulfate-limited conditions

Wu, Songqing; Zhao, Xin; Shen, Hongwei; Wang, Qian; Zhao, Zongbao K.

doi:10.1016/j.biortech.2010.09.033

Cited by 191 publications

(126 citation statements)

References 37 publications

(36 reference statements)

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“…In some cases, SCO presenting composition close to cocoa butter was synthesized, due to the spontaneous ability of yeasts to produce lipids rich in the fatty acids C16:0 and C18:0 [41]. Likewise, R. toruloides strain Y4 has been revealed capable of producing significant lipid quantities during growth on glucose-based media under phosphate- [29] or sulphate- [150] limited conditions. The various nutrient limitations applied induced differences (in some Table 7.…”

Section: Strategies Dealing With the Production Of Cbss By Oleaginousmentioning

confidence: 99%

Lipids of oleaginous yeasts. Part II: Technology and potential applications

Papanikolaou

Aggelis

2011

Euro J Lipid Sci & Tech

347

281

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The process of lipid accumulation in the oleaginous yeasts cultivated in various fermentation configurations when either sugars and related compounds or hydrophobic substances are used as substrates is presented and kinetic models describing both de novo and ex novo lipid accumulation are analyzed. Technological aspects related with single cell oil (SCO) produced by oleaginous yeasts are depicted. The influence of culture parameters upon lipid production process is presented. Lipid production has been studied in batch, fed-batch, and continuous cultivation systems using yeasts belonging to the species Lipomyces starkeyi, Rhodosporidium toruloides, Apiotrichum curvatum, Candida curvata, Cryptococcus curvatus, Trichosporon fermentans, and Yarrowia lipolytica. The potentiality of yeasts to produce SCO as starting material of 2nd generation biodiesel is indicated and discussed. Of significant importance is also the utilization of yeast lipids as substitutes of high added value exotic fats (e.g., cocoa butter). Lipid produced by the various yeasts presents, in general, similar composition with that of common vegetable oils being composed of unsaturated fatty acids, whereas cocoa butter is principally composed of saturated fatty acids, consequently the various strategies that are followed in order to increase the cellular saturated fatty acid content of the yeast lipid are presented and comprehensively discussed.

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Section: Strategies Dealing With the Production Of Cbss By Oleaginousmentioning

confidence: 99%

Lipids of oleaginous yeasts. Part II: Technology and potential applications

Papanikolaou

Aggelis

2011

Euro J Lipid Sci & Tech

347

281

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“…C/N ratio depends on the nature of the microorganism, the medium composition in which the cultivation is undertaken, and types of carbon and nitrogen sources (Ykema et al 1986). It is reported that lipid accumulation in oleaginous yeasts occurs when the growth medium is replete in carbon source with one limiting element that is usually nitrogen limitation (Wu et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Optimization of cultivation conditions for biotechnological production of lipid by Rhodotorula kratochvilovae (syn, Rhodosporidium kratochvilovae) SY89 for biodiesel preparation

et al. 2017

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Rhodotorula kratochvilovae (syn, Rhodosporidium kratochvilovae) SY89, an oleaginous yeast, isolated from Ethiopian soil, was grown under nitrogen-limited media. The capacity this with respect to biomass production, lipid yield and lipid content was evaluated. The influence of inoculum size, carbon sources, variations in glucose concentration, nitrogen sources, C/N ratio, pH, temperature, agitation, and aeration rate and incubation period were investigated. Inoculum size of 10% v/v, glucose as a carbon source at 50 g/ L glucose, 0.50 g/L yeast extract and 0.31 g/L (NH 4 ) 2 SO 4 , C/ N ratio of 120, pH 5.5, incubation temperature of 30°C, 225 rpm, 0.2 as aeration ratio and 144 h of incubation were found to be optimum conditions for lipid production. Then the yeast was grown in a batch bioreactor by combining the different optimized parameters together. Under the optimized conditions, the yeast gave maximum biomass (15.34 ± 1.47 g/L), lipid yield (8.60 ± 0.81 g/L) and lipid content (56.06 ± 1.70%). The dominant fatty acids exhibited in order of their relative abundance (%w/w), were oleic, palmitic, linoleic, stearic, linolenic and palmitoleic acids. The concentration of saturated and monounsaturated fatty acids adds up 78.63 ± 2.19%. This suggests that this strain could be used as a good feedstock for biodiesel production.

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“…Rhodosporidium toruloides is a nonpathogenic basidiomycetous fungus that can accumulate lipids over 70% of its dry cell weight (DCW) (Ratledge and Wynn, 2002;Li et al, 2007). More importantly, R. toruloides had excellent tolerance towards inhibitory compounds found in biomass hydrolysates (Hu et al, 2009;Zhao et al, 2012 (Wu et al, 2011). ILs were supplemented to the above medium at appropriate concentrations after the medium was sterilized.…”

Section: Introductionmentioning

confidence: 99%