Microbial lipid production by oleaginous yeast Cryptococcus sp. in the batch cultures using corncob hydrolysate as carbon source

Chang, Yi-Huang; Chang, Ku-Shang; Lee, Ching-Fu; Hsu, Chuan-Liang; Huang, Cheng-Wei; Jang, Hung-Der

doi:10.1016/j.biombioe.2014.11.012

Cited by 66 publications

(30 citation statements)

References 32 publications

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“…for cell wall remodeling. However, C. oleaginosus readily metabolizes a wide range of oligo- and monomeric sugars such as cellobiose, sucrose, lactose and glucose, galactose, galacturonic acid as well as N -acetylglycosamine respectively [ 20 – 24 ].…”

Section: General Physiologymentioning

confidence: 99%

Opportunities and challenges in the development of Cutaneotrichosporon oleaginosus ATCC 20509 as a new cell factory for custom tailored microbial oils

et al. 2017

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Cutaneotrichosporon oleaginosus ATCC 20509, previously known as Trichosporon oleaginosus, Cryptococcus curvatus, Apiotrichum curvatum or Candida curvata D is an oleaginous yeast with several favorable qualities: it is fast growing, accumulates high amounts of lipid and has a very broad substrate spectrum. Its resistance to hydrolysis byproducts and genetic accessibility make it a promising cell factory for custom tailored microbial oils. However, literature about this organism is of varying degree of quality. Moreover, due to numerous changes of the species name, reports are highly scattered and poorly cited. This led to a poor integration of the findings into a unified body of knowledge. Particularly, errors in strain name usage and consequently citation are found even in most recent literature. To simplify future work, this review provides an overview of published studies and main findings regarding the metabolic capacities of C. oleaginosus.

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Section: General Physiologymentioning

confidence: 99%

Opportunities and challenges in the development of Cutaneotrichosporon oleaginosus ATCC 20509 as a new cell factory for custom tailored microbial oils

et al. 2017

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“…Furthermore, oleaginous yeasts do not require big areas for cultivation and they can grow in low-cost fermentation media containing waste agricultural materials and some industrial byproducts [12]. For example, organic materials such as raw glycerol, lignocellulosic residues, cheese whey, molasses, palm oil and wheat straw are extensively used as cheap growth substrates in cultures of oleaginous yeasts for microbial lipid production [13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

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et al. 2016

Waste Biomass Valor

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This study was performed to produce lipids from the isolated oleaginous yeast Rhodotorula glutinis SO28 using loquat kernel extract (LKE) as substrate. LKE was prepared using acid hydrolysis and alkaline neutralization steps. Lipid production was performed in shaking flaks culture. Even if LKE was used as a sole source of nutritional substances, it could support cell growth and lipid synthesis in the yeast. Additional carbon, nitrogen and phosphorus sources were found to significantly alter the lipid accumulation potential of the yeast. Optimal concentrations of additional carbon (glucose) and nitrogen (ammonium sulphate) sources for lipid accumulation were determined as 15 and 0.5 g/L, respectively. On the other hand, all the concentrations of additional phosphorus source were found to significantly reduce the lipid accumulation. Optimal incubation time was determined as 132 h. Under the optimized culture conditions, the lipid concentration and lipid content of the yeast were determined as 7.82 g/L and 62 %, respectively. Fatty acid methyl ester analysis exhibited that this yeast strain could produce high proportions of C16:0 and C18 fatty acids, which are ideal for biodiesel production. This is the first report on the use of waste loquat kernels as substrate for microbial lipid production.

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“…Farell et al [ 23 ] estimated that first generation biofuel (corn ethanol) can reduce GHG emissions by 18 %, while the second generation biofuel from lignocellulosic feedstocks is expected to reduce emissions by 88 % relative to petroleum-based fuels. The lignocellulosic hydrolysates derived from different biomass feedstocks have been investigated for lipid production in batch cultures [ 10 , 24 – 27 ]. Although promising results were achieved from these studies, the lipid productivity was not sufficient for industrial application.…”

Section: Introductionmentioning

confidence: 99%

Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source

Fei

O’Brien

Nelson

et al. 2016

Biotechnol Biofuels

128

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BackgroundIndustrial biotechnology that is able to provide environmentally friendly bio-based products has attracted more attention in replacing petroleum-based industries. Currently, most of the carbon sources used for fermentation-based bioprocesses are obtained from agricultural commodities that are used as foodstuff for human beings. Lignocellulose-derived sugars as the non-food, green, and sustainable alternative carbon sources have great potential to avoid this dilemma for producing the renewable, bio-based hydrocarbon fuel precursors, such as microbial lipid. Efficient bioconversion of lignocellulose-based sugars into lipids is one of the critical parameters for industrial application. Therefore, the fed-batch cultivation, which is a common method used in industrial applications, was investigated to achieve a high cell density culture along with high lipid yield and productivity.ResultsIn this study, several fed-batch strategies were explored to improve lipid production using lignocellulosic hydrolysates derived from corn stover. Compared to the batch culture giving a lipid yield of 0.19 g/g, the dissolved-oxygen-stat feeding mode increased the lipid yield to 0.23 g/g and the lipid productivity to 0.33 g/L/h. The pulse feeding mode further improved lipid productivity to 0.35 g/L/h and the yield to 0.24 g/g. However, the highest lipid yield (0.29 g/g) and productivity (0.4 g/L/h) were achieved using an automated online sugar control feeding mode, which gave a dry cell weight of 54 g/L and lipid content of 59 % (w/w). The major fatty acids of the lipid derived from lignocellulosic hydrolysates were predominately palmitic acid and oleic acid, which are similar to those of conventional oilseed plants.ConclusionsOur results suggest that the fed-batch feeding strategy can strongly influence the lipid production. The online sugar control feeding mode was the most appealing strategy for high cell density, lipid yield, and lipid productivity using lignocellulosic hydrolysates as the sole carbon source.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0542-x) contains supplementary material, which is available to authorized users.

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Microbial lipid production by oleaginous yeast Cryptococcus sp. in the batch cultures using corncob hydrolysate as carbon source

Cited by 66 publications

References 32 publications

Opportunities and challenges in the development of Cutaneotrichosporon oleaginosus ATCC 20509 as a new cell factory for custom tailored microbial oils

Opportunities and challenges in the development of Cutaneotrichosporon oleaginosus ATCC 20509 as a new cell factory for custom tailored microbial oils

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source

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