Microbial Lipid Accumulation Capability of Activated Sludge Feeding on Short Chain Fatty Acids as Carbon Sources through Fed-Batch Cultivation

Fortela, Dhan Lord; Hern, Rafael; ez,; Zappi, Mark E.; French, Todd W; Bajpai, Rakesh; Chistoserdov, Andrei Y.; Revellame, Emmanuel; Holmes, William E.

doi:10.4172/2155-9821.1000275

Cited by 4 publications

(14 citation statements)

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“…This corresponded to a lipid content of 7.34 wt% of dry biomass, which is more than 5X higher than the lipid content of the seed sludge. However, this lipid content is lower than the results of previous studies that utilized glucose 9−11 and SCCAs 20,21,32 as substrates for lipid accumulation in ASMC. Moreover, as shown in Table 3, this study resulted in lipid yield coefficient, Y x/S that is comparable to that of pure sugar substrates (glucose and xylose), but lower than that of acetic acid.…”

Section: Monomerscontrasting

confidence: 70%

“…Among the three carboxylic acids, acetic acid was preferred by the consortium and resulted in lipid contents between 10 and 20 wt% of dry biomass if used as the sole carbon source. 20,21 SCCAs (i.e., acetic, butyric, lactic, propionic, valeric, citric, succinic, etc.) can be produced through anaerobic fermentation.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Utilization of SCCAs as substrates for lipid production using pure cultures of Cryptococcus curvatus and Cryptococcus albidus showed lipid content reaching as high as 41 and 55 wt% of dry biomass, respectively. , Studies also showed that a mixture of acetic, propionic, and butyric acids could be utilized by ASMC as a carbon source for lipid production. Among the three carboxylic acids, acetic acid was preferred by the consortium and resulted in lipid contents between 10 and 20 wt% of dry biomass if used as the sole carbon source. , …”

Section: Introductionmentioning

confidence: 99%

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Microbial Lipid Production through Integrated Anaerobic-Aerobic Biotreatment Process: An Urban-Based Biorefinery Concept

Revellame

Zappi

Hernández

et al. 2021

ACS Sustainable Chem. Eng.

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Current operations within wastewater treatment plants (WWTPs), particularly the anaerobic digestion and activated sludge processes, can be tapped for production of microbial lipids. This can be accomplished by directing the anaerobic digestion to produce short chain carboxylic acids (SCCAs) that can then be used as substrates for lipid production using activated sludge microbial consortium in an add-on aerobic lipid production unit (similar to an activated sludge process). This study was conducted to assess the suitability of a Test Medium (previously proven suitable for aerobic lipid production in activated sludge) for the proposed integrated system. Results showed that, when used for anaerobic fermentation, the Test medium produced 0.48 g-SCCAs/g-substrate that includes acetic, butyric, and predominantly (∼89 wt%), lactic acid. Subsequently, the Test Medium resulted to a peak lipid yield of ∼0.17 g/g-SCCAs. Complete substrate consumptions were observed without pH control when the Test Medium was used for both bioprocesses. These results suggest the suitability of the Test Medium for the integrated system for microbial lipids production. This could significantly simplify the implementation of the proposed integrated system, which could provide the basis for future urban biorefineries through existing WWTPs.

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Section: Monomerscontrasting

confidence: 70%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microbial Lipid Production through Integrated Anaerobic-Aerobic Biotreatment Process: An Urban-Based Biorefinery Concept

Revellame

Zappi

Hernández

et al. 2021

ACS Sustainable Chem. Eng.

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“…Researchers have shown that activated sludge can be used as a feedstock for biodiesel production. But, due to low yield of 3-6wt%,(drycell weight), which is below the minimum of 10 wt.% (dry cell weight) required for biodiesel to have an economic advantage over the conventional petroleum diesel, this feedstock is still unattractive [60, [73][74]. Another problem is variation in the composition of fatty acids, which depends on the source and composition of wastewater and season of collection, which affect the quality and yield of the biodiesel [5,75].…”

Section: Activated Sludgementioning

confidence: 99%

Biodiesel Production as a Renewable Resource for the Potential Displacement of the Petroleum Diesel

Edeh¹

2020

Biorefinery Concepts, Energy and Products

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In the quest to comply with the Intergovernmental Panel on Climate Change (IPCC) on reducing the global temperature to 1.5-2.0°C as a measure to minimize the climate change caused by the emission of greenhouse gases from the combustion of fossil fuels, and the need for replacement of these fossil fuels, which are also generally believed to be depleting, biodiesel is being studied as a potential replacement for the conventional petroleum diesel. This fuel among other desired properties is renewable, biodegradable, sustainable, and emits less particles. It also contains no amount of sulfur, in addition to possessing most of the good characteristics of petroleum diesel. At the moment, more than 95% of biodiesel produced globally is obtained from vegetable oil feedstocks, which are usually very expensive and thus, without tax waiver and subsidy, makes biodiesel non-competitive with the petroleum diesel. Based on this, non-edible feedstocks are being investigated. Although, their oil yield is low, studies are carried out to ensure efficient extraction. The economics of the process is considered to determine the most economic variables that impact the profitability of biodiesel production. This chapter deals with the biodiesel classification, feedstocks, lipid/oil extraction, biodiesel production methods and the economic aspect of the process.

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“…Researchers have shown that activated sludge can be used as a feedstock for biodiesel production. But, due to low yield of 3-6 wt%, (dry cell weight), which is below the minimum of 10 wt.% (dry cell weight) required for biodiesel to have an economic advantage over the conventional petroleum diesel, this feedstock is still unattractive [60,[73][74]. Another problem is variation in the composition of fatty acids, which depends on the source and composition of wastewater and season of collection, which affect the quality and yield of the biodiesel [5,75].…”

Section: Activated Sludgementioning

confidence: 99%

Biorefinery Concepts, Energy and Products

Beschkov¹

2020

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He received his PhD in 1978 and his DSc degree in 1996. His present interests are chemical and biochemical processes for environment protection and utilization of renewable energy sources. He participated in 36 scientific projects, as well as in 20 applied projects. He has published over 200 scientific papers, 2 monographs, and 7 chapters in selected issues (encyclopedia, books). Over 1500 citations of his papers have been noted. He was Head of the Institute of Chemical Engineering at the Bulgarian Academy of Sciences for 21 years (1993/2014) and Deputy-minister of Environment in the Bulgarian Government (1991/92). ContentsPreface XIII Section 1 General Chapter 1 Biorefinery Safety: A Case Study Focused on Bioethanol Production by Roberto Lauri and Biancamaria Pietrangeli Section 2 Energy and Chemicals Chapter 2 Biogas as a Source of Energy and Chemicals by Sonia Damyanova and Venko Beschkov Chapter 3 Integrated Soybean Biorefinery

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Microbial Lipid Accumulation Capability of Activated Sludge Feeding on Short Chain Fatty Acids as Carbon Sources through Fed-Batch Cultivation

Cited by 4 publications

References 0 publications

Microbial Lipid Production through Integrated Anaerobic-Aerobic Biotreatment Process: An Urban-Based Biorefinery Concept

Microbial Lipid Production through Integrated Anaerobic-Aerobic Biotreatment Process: An Urban-Based Biorefinery Concept

Biodiesel Production as a Renewable Resource for the Potential Displacement of the Petroleum Diesel

Biorefinery Concepts, Energy and Products

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