Microbial lipases: Propitious biocatalysts for the food industry

Salgado, Cleonice Aparecida; Santos, Clarissa Isabela Aparecida dos; Vanetti, Maria Cristina Dantas

doi:10.1016/j.fbio.2021.101509

Cited by 53 publications

(16 citation statements)

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“…(3) palmitoyl, 2-oleoyl,3, (1) stearoyl glycerol and 1, (3) steraroyl, 2oleoyl 3, (1) stearoyl glycerol (Rajendran et al, 2009). In the food processing industry, recombinant lipases are highly encouraged and some have been found with GRAS status and introduced in several food fields, showing that the application of lipases directly to food tends to increase and will be expanded in the coming years (Salgado et al, 2022).…”

Section: Fungal Lipases In Food Processing Industrymentioning

confidence: 99%

Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications

Mahfoudhi

Benmabrouk

Fendri

et al. 2022

Biotech & Bioengineering

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Many researchers have found fungi as a reliable source of lipase due to the versatility of their properties, ease of mass production, thermal stability, pH stability, broad substrate specificity, retained activity in organic solvents, and their low‐cost extraction procedure. This review paper presents an overview about the main aspects of fungal lipases screened from several types of strains as well as their use as biocatalysts. Additionally, some biochemical properties will be reported. As commonly known, lipases can be produced from animals, plants, and microorganisms. Compared to other lipases, those obtained from fungi have been found to be more productive, a fact that encouraged the massive production of most fungal lipases due to their considerable commercial importance during the past few years. This paper is concerned about some of the major characteristics that made fungal lipases desirable products in the industrial fields. Due to the enantioselective properties of fungal lipases and their ability to remain active under extreme temperature, pH, and organic solvents, enzymes are capable to synthesize esters as well as to catalyze a variety of chemical reactions that include esterification, transesterification, acidolysis and aminolysis in aqueous and nonaqueous media. Furthermore, lipases are considered to have a commercial importance for biotechnological application fields, which makes them increasingly popular in food, detergent, cosmetic, organic synthesis, and pharmaceutical domains. The biotechnological potential of lipases has made the latter a coveted choice in industries for the present and future as biocatalysts. In addition, a classification of these fungal enzymes is also highlighted in this review. Moreover, the impact of an immobilization strategy of these fungal strains to achieve higher yields and to improve their production is discussed. Finally, fungal enzymes have played a crucial role from ancient times to today in different fields using several types of biological systems, which gives them a great interest for the production of these enzymes in large amounts with low cost and easy viability to enlarge their use in many industries. Likewise, some future perspectives on lipase production will also be discussed by focusing on special cases on lipase engineering.

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Section: Fungal Lipases In Food Processing Industrymentioning

confidence: 99%

Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications

Mahfoudhi

Benmabrouk

Fendri

et al. 2022

Biotech & Bioengineering

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“…The enzymatic route to ascorbyl palmitate synthesis is catalysed by a lipase. Lipases are quite versatile catalysts with numerous advantages that have secured their application in a broad range of reactions of commercial interest [ 7 , 8 ]. These enzymes have been immobilized on a wide variety of supports using different strategies, allowing their stabilization and reuse under operating conditions.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Synthesis of Ascorbyl Palmitate in a Rotating Bed Reactor

et al. 2023

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Ascorbyl palmitate, an ascorbic acid ester, is an important amphipathic antioxidant that has several applications in foods, pharmaceuticals, and cosmetics. The enzymatic synthesis of ascorbyl palmitate is very attractive, but few efforts have been made to address its process scale-up and implementation. This study aimed at evaluating the enzymatic synthesis of ascorbyl palmitate in a rotating basket reactor operated in sequential batches. Different commercial immobilized lipases were tested, and the most suitable reaction conditions were established. Among those lipases studied were Amano Lipase PS, Lipozyme® TL IM, Lipozyme® Novo 40086, Lipozyme® RM IM and Lipozyme® 435. Initially, the enzymes were screened based on previously defined synthesis conditions, showing clear differences in behavior. Lipozyme® 435 proved to be the best catalyst, reaching the highest values of initial reaction rate and yield. Therefore, it was selected for the following studies. Among the solvents assayed, 2-methyl-2-butanol and acetone showed the highest yields, but the operational stability of the catalyst was better in 2-methyl-2-butanol. The tests in a basket reactor showed great potential for large-scale application. Yields remained over 80% after four sequential batches, and the basket allowed for easy catalyst recycling. The results obtained in basket reactor are certainly a contribution to the enzymatic synthesis of ascorbyl palmitate as a competitive alternative to chemical synthesis. This may inspire future cost-effectiveness studies of the process to assess its potential as a viable alternative to be implemented.

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“…Bio-enzyme catalysis exhibits prominent advantages including special selectivity, environmental friendliness and higher catalytic efficiency in comparison with traditional chemical catalysts. 1 Lipase, one of the active catalysts in ester synthesis and transesterification, is widely used in the bio-chemical fields, such as in food and dairy production, pharmaceuticals and agricultural chemistry, oil chemistry, cosmetics industry and detergent industry, 2–5 and also shows good performances in producing biodiesel from soybean oil and methanol. 6 Glucose oxidase (GOx) is another well known industrial enzyme, which has been applied in baking, wine production and gluconic acid production.…”

Section: Introductionmentioning

confidence: 99%