Microbial linguistics: perspectives and applications of microbial cell-to-cell communication

Mitchell, Robert J.; Lee, Sung Kuk; Kim, Tae Sung; Ghim, Cheol-Min

doi:10.5483/bmbrep.2011.44.1.1

Cited by 24 publications

(18 citation statements)

References 189 publications

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“…Intraspecific communication of Gram-negative bacteria is based on the production and perception of acylated homoserine lactones (AHLs) synthesized by LuxI homologs. Increasing intracellular concentrations of diffusible AHLs with higher cell densities are perceived by binding of the AHLs to the cognate receptor (e.g., LuxR in Vibrio fischeri), resulting in activation or inhibition of target gene transcription (6,7). Interspecific communication between different species within one habitat has been demonstrated to depend on the synthesis and detection of the universal autoinducer-2 (AI-2), which can be inhibited by furanones (8,9).…”

Section: Q Uorum Sensing (Qs) Is the Cell-cell Communication Betweenmentioning

confidence: 99%

Novel Reporter for Identification of Interference with Acyl Homoserine Lactone and Autoinducer-2 Quorum Sensing

Weiland‐Bräuer

Pinnow

Schmitz

2015

Appl Environ Microbiol

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Two reporter strains were established to identify novel biomolecules interfering with bacterial communication (quorum sensing [QS]). The basic design of these Escherichia coli-based systems comprises a gene encoding a lethal protein fused to promoters induced in the presence of QS signal molecules. Consequently, these E. coli strains are unable to grow in the presence of the respective QS signal molecules unless a nontoxic QS-interfering compound is present. The first reporter strain designed to detect autoinducer-2 (AI-2)-interfering activities (AI2-QQ.1) contained the E. coli ccdB lethal gene under the control of the E. coli lsrA promoter. The second reporter strain (AI1-QQ.1) contained the Vibrio fischeri luxI promoter fused to the ccdB gene to detect interference with acyl-homoserine lactones. Bacteria isolated from the surfaces of several marine eukarya were screened for quorum-quenching (QQ) activities using the established reporter systems AI1-QQ.1 and AI2-QQ.1. Out of 34 isolates, two interfered with acylated homoserine lactone (AHL) signaling, five interfered with AI-2 QS signaling, and 10 were demonstrated to interfere with both signal molecules. Open reading frames (ORFs) conferring QQ activity were identified for three selected isolates (Photobacterium sp., Pseudoalteromonas sp., and Vibrio parahaemolyticus). Evaluation of the respective heterologously expressed and purified QQ proteins confirmed their ability to interfere with the AHL and AI-2 signaling processes. Q uorum sensing (QS) is the cell-cell communication betweenbacteria that allows the perception of population density by small signaling molecules-so-called autoinducers-and modifies gene expression in response to the population density. It controls a wide spectrum of processes and phenotypic behaviors, including stress resistance, production of toxins and secondary metabolites, pathogenicity, swarming, and biofilm formation (for a review, see references 1 and 2). In addition to playing important roles in intraspecies and interspecies communication, QS is also involved in host-microbe interactions (3-5). Intraspecific communication of Gram-negative bacteria is based on the production and perception of acylated homoserine lactones (AHLs) synthesized by LuxI homologs. Increasing intracellular concentrations of diffusible AHLs with higher cell densities are perceived by binding of the AHLs to the cognate receptor (e.g., LuxR in Vibrio fischeri), resulting in activation or inhibition of target gene transcription (6, 7). Interspecific communication between different species within one habitat has been demonstrated to depend on the synthesis and detection of the universal autoinducer-2 (AI-2), which can be inhibited by furanones (8, 9). The perception of these signal molecules is achieved either by a two-component regulatory system (e.g., in Vibrio harveyi, where the induced phosphorylation cascade finally results in the induction of specific target genes) (10), or by transportation of the signal molecule into the cell (e.g., in Escherichia coli,...

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Section: Q Uorum Sensing (Qs) Is the Cell-cell Communication Betweenmentioning

confidence: 99%

Novel Reporter for Identification of Interference with Acyl Homoserine Lactone and Autoinducer-2 Quorum Sensing

Weiland‐Bräuer

Pinnow

Schmitz

2015

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…In response to multiple environmental factors, microbes secrete chemical signals, known as autoinducers or quorum-sensing molecules, which regulate the expression of microbial genes involved in biofilm formation, persistence, virulence, and other life cycle processes (21,22). Given the importance of these molecules in pathogenicity, there is strong biological plausibility that such molecules are sensed and responded to by the host.…”

Section: Introductionmentioning

confidence: 99%

T2R38 taste receptor polymorphisms underlie susceptibility to upper respiratory infection

Lee

Xiong²,

Kofonow³

et al. 2012

J. Clin. Invest.

489

1,003

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Innate and adaptive defense mechanisms protect the respiratory system from attack by microbes. Here, we present evidence that the bitter taste receptor T2R38 regulates the mucosal innate defense of the human upper airway. Utilizing immunofluorescent and live cell imaging techniques in polarized primary human sinonasal cells, we demonstrate that T2R38 is expressed in human upper respiratory epithelium and is activated in response to acyl-homoserine lactone quorum-sensing molecules secreted by Pseudomonas aeruginosa and other gram-negative bacteria. Receptor activation regulates calcium-dependent NO production, resulting in stimulation of mucociliary clearance and direct antibacterial effects. Moreover, common polymorphisms of the TAS2R38 gene were linked to significant differences in the ability of upper respiratory cells to clear and kill bacteria. Lastly, TAS2R38 genotype correlated with human sinonasal gram-negative bacterial infection. These data suggest that T2R38 is an upper airway sentinel in innate defense and that genetic variation contributes to individual differences in susceptibility to respiratory infection.

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“…Within many bacteria, fatty acids are usually used to synthesize fatty acyl-homoserine lactone signals that they use for cell-to-cell communication. The process and components involved in bacterial communication with each other, current techniques and applications within labs, as well as a discussion about the purpose of cell-to-cell signaling amongst bacteria was recently reviewed by our group and provides much more information about this remarkable facet of microbiology (47). The photosynthetic bacterium, Rhodopseudomonas palustris includes a luxI-luxR type homologous pair http://bmbreports.org BMB Reports in its chromosome, designated rpaI-rpaR.…”

Section: Introductionmentioning

confidence: 99%

Biological activities of lignin hydrolysate-related compounds

Lee¹,

Monnappa²,

Mitchell³

2012

BMB Reports

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Microbial linguistics: perspectives and applications of microbial cell-to-cell communication

Cited by 24 publications

References 189 publications

Novel Reporter for Identification of Interference with Acyl Homoserine Lactone and Autoinducer-2 Quorum Sensing

Novel Reporter for Identification of Interference with Acyl Homoserine Lactone and Autoinducer-2 Quorum Sensing

T2R38 taste receptor polymorphisms underlie susceptibility to upper respiratory infection

Biological activities of lignin hydrolysate-related compounds

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