Microbial Leakage of Gutta-percha and Resilon™ Root Canal Filling Material: A Comparative Study Using a new Homogeneous Assay for Sequence Detection

Abstract

The sealing ability of gutta-percha/sealer root canal filling was compared to a new thermoplastic synthetic polymer-based obturation material (Resilon TM ), using a microleakage model and a new sequence detection assay One Cut Event AmplificatioN (OCEAN TM ). Eighty-eight extracted human teeth, shaped with K-Files and the ProTaper Technique, were randomly assigned to four groups (n ¼ 22) and obturated in the apical 5 mm. Group R were obturated with the Resilon/Epiphany technique; group GP were obturated with guttapercha and Zinc oxide eugenoe sealer; group RCH and GPCH received calcium hydroxide intracanal medication before being obturated. Sterilized specimens were inoculated with Enterococcus faecalis and incubated in sterile medium for 47 days. DNA extracted from the specimens was amplified by PCR and then identified by the OCEAN technique. Samples obturated with Resilon root canal *Author to whom correspondence should be addressed. E-mail: dampasq@libero.it

In Vitro Effects of External Pressure Changes on the Sealing Ability under Simulated Diving Conditions

“…In addition, De-Deus et al were able to show an increased permeability of Resilon fillings after stress exposure [21]. An increase in external pressure is a type of mechanical stress and our results thus add to existing knowledge about the resistance of Resilon to stress [22, 23]. …”

Dichtigkeit von Wurzelkanalfüllungen mit GuttaFlow® und Resilon™ im Vergleich zur lateralen Kondensation

“…Es gibt aber auch andere Studien, bei denen Epiphany/Resilon TM nicht signifikant besser [30] oder sogar schlecht abschnitt: AH Plus ließ weniger Glukosepenetration zu als Resilon [9,29].…”

Root canal filling using Resilon: a review

“…[80][81][82][83][84][85][86][87][88][89][90] However, analysis of leakage studies must be carried out with caution as a 'superior' result has a different meaning depending on the technique employed. For example, in a dye penetration test a published; these studies form the bulk of the scientific research on Resilon.…”